丹参多酚酸盐对人血管内皮细胞迁移的影响

doi:10.3736/jcim20030320

Abstract

Objective

To study the effect of salvianolate on the migration of endothelial cells induced by monocytes.

Methods

Transwell-boydom system was used to test the migration of endothelial cells induced by monocytes. Enzyme-linked immunosorbent assay (ELISA) and RT-polymerase chain reaction (RT-PCR) method were used to determine the effect of salvianolate on the expression of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF)and their mRNA of monocytes.

Results

The migration of endothelial cells induced by monocytes was facilitated by salvianolate, compared with the control. After the monocytes were treated by salvianolate for 24 hours, the expression of mRNA of VEGF and bFGF was significantly up-regulated, and the expression of VEGF and bFGF was increased.

Conclusion

The facilitation of salvianolate on the migration of endothelial cells induced by monocytes was observed. Salvianolate stimulates the expression of VEGF and bFGF and their mRNA of monocytes, and salvianolate may have the role of inducing migration of endothelial cells by working on these two factors.

Key words: salvianolate, monocytes, endothelium, cell movement, endothelial cell-derived growth factors, basic fibroblast growth factor

CLC Number:

R286.2

Jie Xu, Wei-hu Fan. Effect of salvianolate on migration of human vascular endothelial cells. Journal of Chinese Integrative Medicine, 2003, 1(3): 211-214.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20030320

http://www.jcimjournal.com/EN/Y2003/V1/I3/211

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组别	n	迁移数	迁移促进率（%）
生理盐水对照组	8	48±6
0.25 g/L丹参多酚酸盐组	8	60±9	25
0.5 g/L丹参多酚酸盐组	8	69±11*	44
1 g/L丹参多酚酸盐组	8	75±13*	56

组别	n	VEGF	bFGF
生理盐水对照组	8	37.9±5.2	4.6±1.2
0.25 g/L丹参多酚酸盐组	8	53.5±6.3	8.6±1.9
0.5 g/L丹参多酚酸盐组	8	59.4±6.9*	12.3±2.4*
1 g/L丹参多酚酸盐组	8	66.3±7.1**	13.4±2.8*

Effect of salvianolate on migration of human vascular endothelial cells

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