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Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (4): 273-276.doi: 10.3736/jcim20030413

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Regulation effects of Gegen recipe and Guizhi recipe on expression of IL-1β, iNOS, TNFα, TGFβ mRNA in degenerated cervical intervertebral disk in rabbits

Yong-jun Wang, Qi Shi, Chong-jian Zhou, Bao-xing Hou, Mei Liu, Wen Mo   

  1. Institute of Spinal Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2003-09-04 Online:2003-12-20 Published:2003-12-20

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Objective

To study the effects of Gegen Recipe and Guizhi Recipe regulating the expression of IL-1β, iNOS, TNFα and TGFβ mRNA in degenerated intervertebral disk caused by stimulation of wind, cold and damp.


Methods

Thirty-six rabbits were randomly separated into six groups, viz. control group, stimulation by wind, cold and damp group, head in low-location group, compound model group(head in low-location plus stimulation of wind, cold and damp), Gegen Recipe group and Guizhi Recipe group. The gene expression of IL-1β, iNOS, TNFα and TGFβ mRNA in degenerated cervical intervertebral disks were assayed by RT-PCR.


Results

Compared with the control group, IL-1β mRNA and iNOS mRNA expression were up-regulated in each model group (P<0.01). Both Gegen Recipe and Guizhi Recipe down-regulated IL-1β mRNA, iNOS mRNA expression, which had significant differences compared with the stimulation model group (P<0.01 or<0.05). TNFα mRNA expression was significantly enhanced in the compound model group compared with the control group (P<0.01). There was no significant difference between the head in low-location group and the stimulation model group. Gegen Recipe down-regulated TNFα mRNA expression compared with the stimulation group (P<0.01), while the effect of Guizhi Recipe was not significant. Compared with the control group, TGFβ mRNA expression went down in each model group (P<0.01). Gegen Recipe up-regulated TGFβ mRNA expression (P<0.01), while the effect of Guizhi Recipe on adjusting TGFβ mRNA was not significant.


Conclusion

Stimulation by wind, cold and damp, head in low-location, and compound of both can down-regulate the expressions of IL-1β, iNOS, TNFα mRNA, and up-regulate the expression of TGFβ mRNA. These researches certify that these factors take part in the progression of intervertebral disk degeneration. IL-1β, iNOS, TNFα accelerate the degeneration and TGFβ defers the degeneration. Gegen Recipe down-regulates the expression of TNFα mRNA and up-regulates the expression of TGFβ mRNA. The effect on regulating the cell factors expressions by Guizhi Recipe is lower than that of Gegen Recipe.

Key words: drugs, Chinese herbal, cervical vertebrae, intervertebral disk, interleukin-1beta, nitric-oxide synthase, tumor necrosis factor-alpha, etc

CLC Number: 

  • R681.531

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引物名称,片段（bp） 引物序列
IL-1β(305 bp) Forward
Reverse		 5' GGATGACGGCCTGAGAACTT 3'
5' TCACGCAGGACAGGTACAGA 3'
iNOS(231 bp) Forward
Reverse		 5' GAGACGCACAGGCAGAGGTT 3'
5' GGTGACCACAGCCACAGTGA 3'
TGFβ(250 bp) Forward
Reverse		 5' AACTACTGCTTCAGCTCCAC 3'
5' GTAACACGATGGCGAGTGCG 3'
TNFα(340 bp) Forward
Reverse		 5' GCTGAGCCAGCGTGCGAACG 3'
5' CAGGTACTCAGGCTGGTTGA 3'
β-actin(660 bp) Forward
Reverse		 5' ACGATGCTCCAAGAGCTGTT 3'
5' TCAGGCAGCTCATAGCTCTT 3'

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组 别 n IL-1β mRNA iNOS mRNA
正常对照组 6 0.9532±0.0047 0.9199±0.0129
风寒湿颈椎病组 6 1.0413±0.0152** 1.0026±0.0096**
低头位颈椎病组 6 1.0297±0.0313** 1.0049±0.0182**
低头位加风寒湿颈椎病组 6 1.0885±0.0271**△▲▲ 1.0160±0.0078**
葛根汤治疗组 6 0.9908±0.0121△ 0.9437±0.0031△△
桂枝汤治疗组 6 0.9529±0.0025△△ 0.9057±0.0044△△

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组 别 n TNFα mRNA TGFβ mRNA
正常对照组 6 0.8906±0.0211 0.9710±0.0572
风寒湿颈椎病组 6 0.9045±0.0199 0.8384±0.0305**
低头位颈椎病组 6 0.8958±0.0169 0.8254±0.0351**
低头位加风寒湿颈椎病组 6 1.0284±0.0182**△△▲▲ 0.8027±0.0212**
葛根汤治疗组 6 0.8578±0.0294△△ 0.9662±0.0127△△
桂枝汤治疗组 6 0.9819±0.0285△△ 0.8679±0.0158□□
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