[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (4): 274-277.doi: 10.3736/jcim20040412

• Original Experimental Research • Previous Articles     Next Articles

Expression of TIMP-1 and TGF-β1 mRNA in hepatic fibrosis rats and the therapeutic effects of Bushen Rougan Recipe

Bin Zhang1(), Mo-bin Wan1, Lin-tai Wang2   

  1. 1. Department of Infectious Diseases,Changhai Hospital, Second Military Medical University,Shanghai 200433, China
    2. Department of Liver Diseases, Shuguang Hospital,Shanghai University of Traditional Chinese Medicine, Shanghai 200021,China
  • Received:2004-04-19 Online:2004-07-20 Published:2018-10-20

RichHTML

1

PDF (PC)

483

Like

Objective: To study the expression of TIMP-1 and TGF-β1 mRNA in hepatic fibrosis rats and the therapeutic effects of Bushen Rougan Recipe (BSRGR).Methods: Hepatic fibrosis was induced in rats by dimethylnitrosamine (DMN). The rats' hepatic tissue was studied by HE staining and Sirius red staining. The rats were divided into normal control group, fibrosis model group and BSRGR treated group (10 g·kg -1·d -1, i.g. for 4 weeks). At last, TIMP-1 and TGF-β1 mRNA were detected by RT-PCR. Results: Pathological study showed that hepatic fibrosis was successfully induced by DMN in rats. The expression quantity of TIMP-1 and TGF-β1 mRNA were the most in the fibrosis model group, the second in the BSRGR treated group,and the least in the normal control group.Conclusion: The expression of TIMP-1 and TGF-β1mRNA was increased in the hepatic fibrosis rats, and this is one possible mechanism of hepatic fibrosis. BSRGR can inhibit the advancement of hepatic fibrosis.

Key words: hepatic fibrosis, Bushen Rougan Recipe, dimethylnitrosamine, TIMP-1, TGF-β1

CLC Number: 

  • R285.5

Tab 1

Prime sequences for PCR"

Prime Sn An Length （bp）
TIMP-1 CCACAGATATCCGGTTCGCCTACA GCACACCCCACAGCCAGCACTAT 218
TGF-β1 AGGCGGTGCTCGCTTTGT CCCGAATGTCTGACGTATTGAA 201
β-actin TCACCCACACTGTGCCCATCTACGA TCACCCACACTGTGCCCATCTACGA 300

Fig 1

Sirius red staining of liver tissue（×100）"

Tab 2

The degree of liver fibrosis"

Group n Degree of liver fibrosis Ridit analysis
– + ++ +++ ++++
Normal control 10 10 P<0.01
Fibrosis model 9 0 0 4 2 3
BSRGR-treated 12 0 0 6 5 1 P<0.05

Tab 3

Expression level of TIMP-1 and TGF-β1 mRNA ($\bar{x}$±s )"

Group n TIMP-1/β-actin TGF-β1/β-actin
Normal control 3 0.523±0.30** 0.663±0.10**
Fibrosis model 3 0.823±0.45△△ 1.100±0.21△△
BSRGR-treated 3 0.617±0.02**△△ 0.812±0.04**

Fig 2

Agrose gel electrophoresis of TIMP-1 PCR products Lane 1: Marker(3 000, 2 000, 1 500, 1 200, 1 031, 900, 800, 700, 600, 500, 400, 300, 200, 100 bp); Lane 2-4: Amplified products of β-actin (300 bp); Lane 5-7: Amplified products of normal control group, fibro-sis model group and BSRGR treated group, respectively (218 bp)."

Fig 3

Agrose gel electrophoresis of TGF-β1 PCR products Lane 1: Marker (3 000, 2 000, 1 500, 1 200, 1 031, 900, 800, 700, 600, 500, 400, 300, 200, 100 bp); Lane 2-4: Amplified products of β-actin (300 bp); Lane 5-7: Amplified products of normal control group, fibro-sis model group and BSRGR treated group, respectively (201 bp)."

[1] Friedman SL . Molecular mechanisms of hepatic fibrosis and principles of therapy[J]. J Gastroenterol, 1997,32(3):424-430
doi: 10.1007/BF02934504
[2] Lamireau T, Desmouliere A, Bioulac-Sage P , et al. Mechanisms of hepatic fibrogenesis[J]. Arch Pediatr, 2002,9(4):392-405
doi: 10.1016/S0929-693X(01)00800-4
[3] Housset C, Guechot J . Hepatic fibrosis:physiopathology and biological diagnosis[J]. Pathol Biol(Paris), 1999,47(9):886-894
pmid: 10609268
[4] Albanis E, Friedman SL . Hepatic fibrosis. Pathogenesis and principles of therapy[J]. Clin Liver Dis, 2001,5(2):315-334, v-vi
doi: 10.1016/S1089-3261(05)70168-9
[5] Levy MT, McCaughan GW, Marinos G , et al. Intrahepatic expression of the hepatic stellate cell marker fibroblast activation protein correlates with the degree of fibrosis in hepatitis C virus infection[J]. Liver, 2002,22(2):93-101
doi: 10.1034/j.1600-0676.2002.01503.x
[6] Liu P, Liu CH, Wang HN , et al. Effect of salvianolic acid B on collagen production and mitogen-activated protein kinase activity in rat hepatic stellate cells[J]. Acta Pharmacol Sin, 2002,23(8):733-738
[7] Brunt EM . Grading and staging the histopathological lesions of chronic hepatitis: the Knodell histology activity index and beyond[J]. Hepatology, 2000,31(1):241-246
doi: 10.1002/(ISSN)1527-3350
[8] Inuzuka S, Ueno T, Torimura T , et al. The significance of colocalization of plasminogen activator inhibitor-1 and vitronection in hepatic fibrosis[J]. Scand J Gastroenterol, 1997,32(10):1052-1060
doi: 10.3109/00365529709011224
[9] 张杰, 徐列明 . 中医药治疗实验性肝纤维化作用机制研究进展[J]. 中西医结合学报, 2003,1(2):142-145
doi: 10.3969/j.issn.1009-3427.2003.04.008
Zhang J, Xu LM . Advances of mechanism research on treatment of experimental liver fibrosis with traditional Chinese medicine[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(2):142-145
doi: 10.3969/j.issn.1009-3427.2003.04.008
[10] Kahari VM, Chen YQ, Su MW , et al. Tumor necrosis factor-alpha and interferon-garmma suppress the activation of human type Ⅰcollagen gene expression by transforming growth factor-beta 1.Evidence for two distinct mechanisms of inhibition at the transcriptional and posttranscriptional levels[J]. J Clin Invest, 1990,86(5):1489-1495
doi: 10.1172/JCI114866
[11] 陶艳艳, 刘成海 . 丹参及其化学成分抗肝纤维化作用机制研究进展[J]. 中西医结合学报, 2004,2(2):145-148
Tao YY, Liu CH . Progress of research on mechanism of salvia miltiorrhiza and its chemical ingredients against liver fibrosis[J]. Zhong Xi Yi Jie He Xue Bao, 2004,2(2):145-148
[12] 刘平, 胡义扬, 刘成 , 等. 扶正化瘀胶囊干预慢性乙型肝炎肝纤维化作用的多中心临床研究[J]. 中西医结合学报, 2003,1(2):89-98, 102
doi: 10.3969/j.issn.1008-861X.2002.01.013
Liu P, Hu YY, Liu C , et al. Multicenter clinical study about the action of Fuzheng Huayu Capsule against liver fibrosis with chronic hepatitis B[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(2):89-98, 102
doi: 10.3969/j.issn.1008-861X.2002.01.013
[13] 刘平 . 理论联系实际, 基础结合临床, 促进中西医结合肝脏病学科发展[J]. 中西医结合学报, 2003,1(2):81-83
[1] Qing-lan Wang, Yan-yan Tao , Li Shen , Hong-yan Cui , Cheng-hai Liu. Chinese herbal medicine Fuzheng Huayu recipe inhibits liver fibrosis by mediating the transforming growth factor-β1/Smads signaling pathway. Journal of Chinese Integrative Medicine, 2012, 10(5): 561-568.
[2] Yan-qin Bian, Bing-bing Ning , Hong-yan Cao , Yan LU , Cheng Liu , Gao-feng Chen , Jia Liu , Ping Liu, Ming-yu Sun. Formula-syndrome correlation study of three classical anti-jaundice formulas in inhibition of liver fibrosis induced by dimethylnitrosamine in rats. Journal of Chinese Integrative Medicine, 2012, 10(12): 1405-1412.
[3] Xiao Zhang , Bing-bing Ning , Shuang Ren , Li-jun Zhang , Wen-meng Zhang , Jia-mei Chen , Gao-feng Chen , Hua Zhang , Yong-ping Mu , Ping Liu. Effects of Chinese herbal medicine Xiaopi Pill in preventing rats from dimethylnitrosamine-induced liver fibrosis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1286-1292.
[4] Xiao-ning Wang, Qing Tao, Qin Feng, Jing-hua Peng, Ping Liu, Yi-yang Hu. Effects of Chinese herbal medicine Yiguanjian Decoction on collagen metabolism of hepatic tissues in rats with CCI4-induced liver fibrosis. Journal of Chinese Integrative Medicine, 2011, 9(6): 651-657.
[5] Ming-yu Sun, Lei Wang, Yong-ping Mu, Cheng Liu, Yan-qin Bian, Xiao-ning Wang, Ping Liu. Effects of Chinese herbal medicine Yinchenhao Decoction on expressions of apoptosis-related genes in dimethylnitrosamine- or carbon tetrachloride-induced liver cirrhosis in rats. Journal of Chinese Integrative Medicine, 2011, 9(4): 423-434.
[6] Bin Zhang, Ling-tai Wang. Bushen Rougan Recipe in prevention of hepatic fibrosis in rats induced by dimethylnitrosamine: a study on its preliminary mechanism. Journal of Chinese Integrative Medicine, 2008, 6(9): 934-938.
[7] Chinese Association of Integrative Medicine, Liver Disease Committee. Guideline for the diagnosis and treatment of liver fibrosis with integrative medicine. Journal of Chinese Integrative Medicine, 2006, 4(6): 551-555.
[8] Xia Hu, Yue-jin Zhou, Qing-rong Xie, Xiao-cai Sun, Yang Xiao. Clinical observation of sophocarpidine combined with Salviae miltiorrhizae injection in treating chronic hepatitis B. Journal of Chinese Integrative Medicine, 2006, 4(6): 648-649.
[9] Chang-qing Zhao​, Yi-qing Wu​, Lie-ming Xu​. Curative effects of Fuzheng Huayu Capsules on hepatic fibrosis and the functional mechanisms: A review. Journal of Chinese Integrative Medicine, 2006, 4(5): 467-472.
[10] Feng-hua Li​, Ping Liu​, Wei-guo Xiong​, Guang-fu Xu​. Effects of Cordyceps sinensis on dimethylnitrosamine-induced liver fibrosis in rats. Journal of Chinese Integrative Medicine, 2006, 4(5): 514-517.
[11] Shan-zhong Tan​, Cheng-hai Liu​, Wei Zhang​, Xiong Lu​, Wei-cheng Ye​, Zhen-zhen Cai​, Ping Liu​. Feature changes of MMP-2/9 activities and TIMP-1/2 protein expressions during the progression of pulmonary fibrosis in rats. Journal of Chinese Integrative Medicine, 2006, 4(4): 402-407.
[12] Lei Wang, Guang Ji, Pei-yong Zheng, Ai-hua Long. Establishment of a rat model of alcoholic liver fibrosis induced by complex factors. Journal of Chinese Integrative Medicine, 2006, 4(3): 281-284.
[13] Bin Zhang​, Lin-tai Wang​. Progress in experimental study on inhibitory effect of traditional Chinese medicine on liver fibrosis. Journal of Chinese Integrative Medicine, 2006, 4(2): 206-210.
[14] Xiao-ning Wang, Yi-yang Hu, Cheng-hai Liu, Ping Liu, Da-yuan Zhu. Effects of salvianolic acid B on expressions of TGF-β1 and its receptors in liver of rats with dimethylnitrosamine-induced hepatic fibrosis. Journal of Chinese Integrative Medicine, 2005, 3(4): 286-288.
[15] Bin Zhang, Mo-bin Wan, Ling-tai Wang. Therapeutic effect of Bushen Rougan Recipe on hepatic fibrosis in rats. Journal of Chinese Integrative Medicine, 2005, 3(2): 132-135.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10] Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022
[an error occurred while processing this directive]