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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (4): 274-277.doi: 10.3736/jcim20040412

• Original Experimental Research • Previous Articles     Next Articles

Expression of TIMP-1 and TGF-β1 mRNA in hepatic fibrosis rats and the therapeutic effects of Bushen Rougan Recipe

Bin Zhang1(), Mo-bin Wan1, Lin-tai Wang2   

  1. 1. Department of Infectious Diseases,Changhai Hospital, Second Military Medical University,Shanghai 200433, China
    2. Department of Liver Diseases, Shuguang Hospital,Shanghai University of Traditional Chinese Medicine, Shanghai 200021,China
  • Received:2004-04-19 Online:2004-07-20 Published:2018-10-20

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Objective: To study the expression of TIMP-1 and TGF-β1 mRNA in hepatic fibrosis rats and the therapeutic effects of Bushen Rougan Recipe (BSRGR).Methods: Hepatic fibrosis was induced in rats by dimethylnitrosamine (DMN). The rats' hepatic tissue was studied by HE staining and Sirius red staining. The rats were divided into normal control group, fibrosis model group and BSRGR treated group (10 g·kg -1·d -1, i.g. for 4 weeks). At last, TIMP-1 and TGF-β1 mRNA were detected by RT-PCR. Results: Pathological study showed that hepatic fibrosis was successfully induced by DMN in rats. The expression quantity of TIMP-1 and TGF-β1 mRNA were the most in the fibrosis model group, the second in the BSRGR treated group,and the least in the normal control group.Conclusion: The expression of TIMP-1 and TGF-β1mRNA was increased in the hepatic fibrosis rats, and this is one possible mechanism of hepatic fibrosis. BSRGR can inhibit the advancement of hepatic fibrosis.

Key words: hepatic fibrosis, Bushen Rougan Recipe, dimethylnitrosamine, TIMP-1, TGF-β1

CLC Number: 

  • R285.5

Tab 1

Prime sequences for PCR"

Prime Sn An Length （bp）
TIMP-1 CCACAGATATCCGGTTCGCCTACA GCACACCCCACAGCCAGCACTAT 218
TGF-β1 AGGCGGTGCTCGCTTTGT CCCGAATGTCTGACGTATTGAA 201
β-actin TCACCCACACTGTGCCCATCTACGA TCACCCACACTGTGCCCATCTACGA 300

Fig 1

Sirius red staining of liver tissue（×100）"

Tab 2

The degree of liver fibrosis"

Group n Degree of liver fibrosis Ridit analysis
– + ++ +++ ++++
Normal control 10 10 P<0.01
Fibrosis model 9 0 0 4 2 3
BSRGR-treated 12 0 0 6 5 1 P<0.05

Tab 3

Expression level of TIMP-1 and TGF-β1 mRNA ($\bar{x}$±s )"

Group n TIMP-1/β-actin TGF-β1/β-actin
Normal control 3 0.523±0.30** 0.663±0.10**
Fibrosis model 3 0.823±0.45△△ 1.100±0.21△△
BSRGR-treated 3 0.617±0.02**△△ 0.812±0.04**

Fig 2

Agrose gel electrophoresis of TIMP-1 PCR products Lane 1: Marker(3 000, 2 000, 1 500, 1 200, 1 031, 900, 800, 700, 600, 500, 400, 300, 200, 100 bp); Lane 2-4: Amplified products of β-actin (300 bp); Lane 5-7: Amplified products of normal control group, fibro-sis model group and BSRGR treated group, respectively (218 bp)."

Fig 3

Agrose gel electrophoresis of TGF-β1 PCR products Lane 1: Marker (3 000, 2 000, 1 500, 1 200, 1 031, 900, 800, 700, 600, 500, 400, 300, 200, 100 bp); Lane 2-4: Amplified products of β-actin (300 bp); Lane 5-7: Amplified products of normal control group, fibro-sis model group and BSRGR treated group, respectively (201 bp)."

[1] Friedman SL . Molecular mechanisms of hepatic fibrosis and principles of therapy[J]. J Gastroenterol, 1997,32(3):424-430
doi: 10.1007/BF02934504
[2] Lamireau T, Desmouliere A, Bioulac-Sage P , et al. Mechanisms of hepatic fibrogenesis[J]. Arch Pediatr, 2002,9(4):392-405
doi: 10.1016/S0929-693X(01)00800-4
[3] Housset C, Guechot J . Hepatic fibrosis:physiopathology and biological diagnosis[J]. Pathol Biol(Paris), 1999,47(9):886-894
pmid: 10609268
[4] Albanis E, Friedman SL . Hepatic fibrosis. Pathogenesis and principles of therapy[J]. Clin Liver Dis, 2001,5(2):315-334, v-vi
doi: 10.1016/S1089-3261(05)70168-9
[5] Levy MT, McCaughan GW, Marinos G , et al. Intrahepatic expression of the hepatic stellate cell marker fibroblast activation protein correlates with the degree of fibrosis in hepatitis C virus infection[J]. Liver, 2002,22(2):93-101
doi: 10.1034/j.1600-0676.2002.01503.x
[6] Liu P, Liu CH, Wang HN , et al. Effect of salvianolic acid B on collagen production and mitogen-activated protein kinase activity in rat hepatic stellate cells[J]. Acta Pharmacol Sin, 2002,23(8):733-738
[7] Brunt EM . Grading and staging the histopathological lesions of chronic hepatitis: the Knodell histology activity index and beyond[J]. Hepatology, 2000,31(1):241-246
doi: 10.1002/(ISSN)1527-3350
[8] Inuzuka S, Ueno T, Torimura T , et al. The significance of colocalization of plasminogen activator inhibitor-1 and vitronection in hepatic fibrosis[J]. Scand J Gastroenterol, 1997,32(10):1052-1060
doi: 10.3109/00365529709011224
[9] 张杰, 徐列明 . 中医药治疗实验性肝纤维化作用机制研究进展[J]. 中西医结合学报, 2003,1(2):142-145
doi: 10.3969/j.issn.1009-3427.2003.04.008
Zhang J, Xu LM . Advances of mechanism research on treatment of experimental liver fibrosis with traditional Chinese medicine[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(2):142-145
doi: 10.3969/j.issn.1009-3427.2003.04.008
[10] Kahari VM, Chen YQ, Su MW , et al. Tumor necrosis factor-alpha and interferon-garmma suppress the activation of human type Ⅰcollagen gene expression by transforming growth factor-beta 1.Evidence for two distinct mechanisms of inhibition at the transcriptional and posttranscriptional levels[J]. J Clin Invest, 1990,86(5):1489-1495
doi: 10.1172/JCI114866
[11] 陶艳艳, 刘成海 . 丹参及其化学成分抗肝纤维化作用机制研究进展[J]. 中西医结合学报, 2004,2(2):145-148
Tao YY, Liu CH . Progress of research on mechanism of salvia miltiorrhiza and its chemical ingredients against liver fibrosis[J]. Zhong Xi Yi Jie He Xue Bao, 2004,2(2):145-148
[12] 刘平, 胡义扬, 刘成 , 等. 扶正化瘀胶囊干预慢性乙型肝炎肝纤维化作用的多中心临床研究[J]. 中西医结合学报, 2003,1(2):89-98, 102
doi: 10.3969/j.issn.1008-861X.2002.01.013
Liu P, Hu YY, Liu C , et al. Multicenter clinical study about the action of Fuzheng Huayu Capsule against liver fibrosis with chronic hepatitis B[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(2):89-98, 102
doi: 10.3969/j.issn.1008-861X.2002.01.013
[13] 刘平 . 理论联系实际, 基础结合临床, 促进中西医结合肝脏病学科发展[J]. 中西医结合学报, 2003,1(2):81-83
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