[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (4): 285-.doi: 10.3736/jcim20040416

• Original Experimental Research • Previous Articles     Next Articles

Experimental study of Qiyeling Decoction inducing apoptosis of transplanted human lung adenocarcinoma cells A549 in nude mice

Chang-juan Jin1, Hui-fang Sha2, Lan-xiang Zhao2, Jiu-xian Feng2, Wei-yong Gu2, Zheng-yu Shi2   

  1. 1. Department of Integrated Traditional Chinese and Western Medicine, Shanghai Chest Hospital, Shanghai 200030, China
    2. Division of Cancer Research, Shanghai Chest Hospital, Shanghai 200030, China
  • Received:2004-05-31 Online:2004-07-20 Published:2018-10-20

RichHTML

1

PDF (PC)

697

Like

Objective: To study the function of Qiyeling Decoction in inducing apoptosis of transplanted human lung adenocarcinoma cells A549 in nude mice.Methods: Nude mice with transplanted A549 tumor were randomly divided into the untreated control group (group A), chemotherapy treated group (group B), chemotherapy plus Qiyeling Decoction treated group (group C), Qiyeling Decoction treated group (group D) and managed correspondingly. The tumor volume was measured and calculated into tumor weight. The apoptosis of tumor cells were examined using in situ cell apoptosis detection kit.Results: The tumor weight was lower obviously in groups B, C and D than that in group A (P<0.05). The apoptosis of tumor cells was lower obviously in groups B, and C than that in group D (P<0.05). Cells in group A appeared perfect differentiation during the early stage and apoptosis later.Conclusion: Qiyeling Decoction can induce A549 cell apoptosis in nude mice.

Key words: Qiyeling Decoction, tumor, apoptosis, animal, laboratory

CLC Number: 

  • R289.5

Tab 1

Tumor weight and apoptosis cell number in 4 groups ($\bar{x}$±s )"

Group n Tumor weight （g） Apoptosis cell number
Untreated control group (A) 12 12.0±3.40 5.12±2.55▲
Chemotherapy treated group (B) 12 8.35±3.37* 3.09±1.41▲
Chemotherapy plus Qiyeling Decoction treated group (C) 12 4,19±2,20*△ 3.96±0.97▲
Qiyeling Decoction treated group (D) 12 2.73±1.87*△ 6.31±1.52

Fig 1

In situ cell apoptosis of tumor tissue in 4 groups of nude mice (×200)"

[1] 杨骅, 王仙平, 郁琳琳 . 榄香烯抗癌作用与诱发肿瘤细胞凋亡[J]. 中华肿瘤杂志, 1996,18(3):169-171
[2] 杨勤建, 涂晋文 . 中药诱导肿瘤细胞凋亡的研究进展[J]. 湖北中医杂志, 1998,20(1):53-55
[3] 韩明权, 刘嘉湘, 高虹 , 等. 益肺抗瘤饮对实验性肺癌细胞周期及核酸和蛋白质合成的影响[J]. 中西医结合学报, 2003,1(3):205-208
Han MQ, Liu JX, Gao H , et al. Effects of Yifei Kangliu Qral Liquid on cell cycle and protein-nucleic acid synjournal of experimental lung cancer[J]. Zhong Xi Yi Jie He Xue Bao, 2003,1(3):205-208
[4] 李蒙, 于建勋 . 单味中药诱导肿瘤细胞凋亡的实验研究现状及展望[J]. 中国中西医结合杂志, 2001,21(1):74-76
doi: 10.7661/CJIM.2001.1.74
[5] 金长娟, 沙慧芳 . 七叶灵对Lewis肺癌细胞荷瘤小鼠作用的初步观察[J]. 上海中医药大学学报, 2002,16(2):54-55
doi: 10.3969/j.issn.1008-861X.2002.02.018
[6] 金长娟, 徐振晔 . 黄精五味方升高白细胞作用临床初步观察[J]. 上海中医药杂志, 1993,27(1):30
[7] 金长娟, 李蕾, 崔清 , 等. 中西药综合治疗晚期非小细胞肺癌的临床观察[J]. 上海中医药杂志, 2003,37(7):16-17
doi: 10.3969/j.issn.1007-1334.2003.07.006
[8] 黎丹戎, 侯华新, 张玮 , 等. 汉黄芩诱导人卵巢癌细胞A2780凋亡及对细胞端粒酶活性的影响[J]. 癌症, 2003,22(8):801-805
doi: 10.3321/j.issn:1000-467X.2003.08.004
[1] Avijit Paul, Kausik Bishayee, Samrat Ghosh, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Chelidonine isolated from ethanolic extract of Chelidonium majus promotes apoptosis in HeLa cells through p38-p53 and PI3K/AKT signalling pathways. Journal of Chinese Integrative Medicine, 2012, 10(9): 1025-1038.
[2] Yong Zhang, Xiao-wen Sun, Jian-hua Xu, Hai Lu, Zhong-ze Fan, Jue Sun, Xiao-xiao Zhang. Effects of medicated serum prepared with Chinese herbal medicine Changweiqing on pharmacokinetics of oxaliplatin in colon cancer cells. Journal of Chinese Integrative Medicine, 2012, 10(8): 901-910.
[3] Samuel Joshua Pragasam, Vachana Murunikara, Evan Prince Sabina, Mahaboobkhan Rasool. Antiperoxidative potential of p-coumaric acid, a common dietary phenol, in adjuvant-induced arthritis in rats. Journal of Chinese Integrative Medicine, 2012, 10(8): 932-938.
[4] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[5] Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu. Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes. Journal of Chinese Integrative Medicine, 2012, 10(7): 821-826.
[6] Yan Guan , Hui Zhang, Wei Zhang, Shi-bing Su . Analysis of differential gene expression profile in peripheral blood of patients with chronic hepatitis B and syndromes of dual deficiency of liver and kidney yin and accumulation of dampness heat. Journal of Chinese Integrative Medicine, 2012, 10(7): 751-756.
[7] Lin Wang, Guan-liang Wang, Jia-han Liu, Di Li, De-zeng Zhu, Liang-neng Wu . Effects of ursolic acid in ameliorating insulin resistance in liver of KKAy mice via peroxisome proliferator-activated receptors α and γ. Journal of Chinese Integrative Medicine, 2012, 10(7): 793-799.
[8] Shui-qiu Deng, Xue-nong Ouyang, Zong-yang Yu, Xi-hu Dai, Xi Chen, Fang-zheng Fang, Wen-wu Wang, Zhi-zhen Liu. Influence of Chinese herbal medicine Feitai Capsule on completion or delay of chemotherapy in patients with stage ⅢB/Ⅳ non-small-cell lung cancer: a Randomized controlled trial. Journal of Chinese Integrative Medicine, 2012, 10(6): 635-640.
[9] Yogesh Bendale, Vineeta Bendale, Saili Paul, Soumya Sundar Bhattacharyya. Green synthesis, characterization and anticancer potential of platinum nanoparticles Bioplatin. Journal of Chinese Integrative Medicine, 2012, 10(6): 681-689.
[10] Ju-yong Wang, Jing Guo, Chang-sheng Dong, Zhan Zheng, Qing Wang. Effects of Feiyanning Decoction, a compound traditional Chinese medicine, on iNOS and COX-2 expressions induced by tumor necrosis factor-α in lung adenocarcinoma cell line. Journal of Chinese Integrative Medicine, 2012, 10(6): 695-700.
[11] Daniel A. Weber, Janelle M. Wheat , Geoffrey M. Currie. Cancer stem cells and the impact of Chinese herbs, isolates and other complementary medical botanicals: A review. Journal of Chinese Integrative Medicine, 2012, 10(5): 493-503.
[12] Qing-bo Lang, Dong-xia Zhai , Feng Huang, Jian-guo Chen, Yong-hui Zhang, Qun Liu, Xiao-feng Zhai , Bai Li, Chang-quan Ling. Investigation on traditional Chinese medicine syndrome distribution of 4 618 hepatitis B virus infection subjects in Qidong of Jiangsu Province, China. Journal of Chinese Integrative Medicine, 2012, 10(5): 525-531.
[13] Gang Guo, Jian-hua Xu, Jian-hong Han, Fang Liang, Yong Zhang, Qiang Zhang, Jue Sun, Zhong-ze Fan . Chinese herbal decoction Shiquan Dabu Tang inhibits tumor growth and angiogenesis of metastasis after primary tumor surgical removal in mice. Journal of Chinese Integrative Medicine, 2012, 10(4): 436-447.
[14] Li Weng, Juan Du, Wen-ring He, Chang-quan Ling. Characteristic genomics of peripheral blood mononuclear cells of hepatocellular carcinoma patients with liver-kidney yin deficiency syndrome. Journal of Chinese Integrative Medicine, 2012, 10(4): 406-415.
[15] Jin-bin Han, Yong-qiang Hua, Lian-yu Chen, Lu-ming Liu . Advances in Smoothened-targeting therapies for pancreatic cancer: Implication for drug discovery from herbal medicines. Journal of Chinese Integrative Medicine, 2012, 10(3): 256-263.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2] Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10] Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239
[an error occurred while processing this directive]