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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (4): 285-.doi: 10.3736/jcim20040416

• Original Experimental Research • Previous Articles     Next Articles

Experimental study of Qiyeling Decoction inducing apoptosis of transplanted human lung adenocarcinoma cells A549 in nude mice

Chang-juan Jin1, Hui-fang Sha2, Lan-xiang Zhao2, Jiu-xian Feng2, Wei-yong Gu2, Zheng-yu Shi2   

  1. 1. Department of Integrated Traditional Chinese and Western Medicine, Shanghai Chest Hospital, Shanghai 200030, China
    2. Division of Cancer Research, Shanghai Chest Hospital, Shanghai 200030, China
  • Received:2004-05-31 Online:2004-07-20 Published:2018-10-20

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Objective: To study the function of Qiyeling Decoction in inducing apoptosis of transplanted human lung adenocarcinoma cells A549 in nude mice.Methods: Nude mice with transplanted A549 tumor were randomly divided into the untreated control group (group A), chemotherapy treated group (group B), chemotherapy plus Qiyeling Decoction treated group (group C), Qiyeling Decoction treated group (group D) and managed correspondingly. The tumor volume was measured and calculated into tumor weight. The apoptosis of tumor cells were examined using in situ cell apoptosis detection kit.Results: The tumor weight was lower obviously in groups B, C and D than that in group A (P<0.05). The apoptosis of tumor cells was lower obviously in groups B, and C than that in group D (P<0.05). Cells in group A appeared perfect differentiation during the early stage and apoptosis later.Conclusion: Qiyeling Decoction can induce A549 cell apoptosis in nude mice.

Key words: Qiyeling Decoction, tumor, apoptosis, animal, laboratory

CLC Number: 

  • R289.5

Tab 1

Tumor weight and apoptosis cell number in 4 groups ($\bar{x}$±s )"

Group n Tumor weight （g） Apoptosis cell number
Untreated control group (A) 12 12.0±3.40 5.12±2.55▲
Chemotherapy treated group (B) 12 8.35±3.37* 3.09±1.41▲
Chemotherapy plus Qiyeling Decoction treated group (C) 12 4,19±2,20*△ 3.96±0.97▲
Qiyeling Decoction treated group (D) 12 2.73±1.87*△ 6.31±1.52

Fig 1

In situ cell apoptosis of tumor tissue in 4 groups of nude mice (×200)"

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