[an error occurred while processing this directive]

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (3): 211-215.doi: 10.3736/jcim20050313

• Original Experimental Research • Previous Articles     Next Articles

Impact of antipyretic and purgative herbs on intestinal mucosal barrier and inflammatory response in treatment of acute cholangitis in rats

Jing-zhe Zhang,Xue-lin Zhang,Ju Gao,Ping Shen,En-wei Ma,Hong-gang Gu,Pei-ting Zhu   

  1. Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2005-04-01 Online:2005-05-20 Published:2005-05-20
  • Contact: Jing-zhe Zhang E-mail:zjzzzq@sina.com

RichHTML

4

PDF (PC)

277

Cited

10

Like

Objective

To investigate the effects of antipyretic and purgative herbs on intestinal mucosal barrier and inflammatory response in the treatment of acute cholangitis.

Methods

Sixty SD rats were randomly divided into group A (untreated group, acute cholangitis was induced, n=20), group B (treatment group, acute cholangitis was induced and treated with antipyretic and purgative herbs, n=20) and group C (sham operation group, n=20). At the third or fifth day after operation, the rats were sacrificed and sampled. The serum endotoxin, cytokines and inflammatory mediators were tested and the numbers of labeled bacteria in the liver, spleen and mesenteric lymph nodes translocated from the gut were assayed.

Results

As compared with group A, the serum content of endotoxin, IL-6, IL-8, TNF-α, CRP and NO was significantly lower and that of IL-2 was significantly higher, and the translocated numbers of labeled bacteria from gut were reduced in both group B and group C（P<0.01).

Conclusion

Antipyretic and purgative herbs can play therapeutic roles in the treatment of acute biliary tract infections, including the protection of intestinal mucosal barrier from bacterial translocation, reduction of serum endotoxin content and regulation of inflammatory response.

Key words: Antipyretic and purgative herbs, Cholangitis, Cytokines, Inflammatory mediators

CLC Number: 

  • R283.624

Tab 1

Serum content of cytokines in each group on the third experiment day ($\bar{x}$±s)"

Group n TNF (ng/L) IL-2 (ng/L) IL-6 (μg/L) IL-8 (μg/L)
Group A 8 51.04±10.10** 28.47±2.98** 225.92±48.41** 922.13±192.53**
Group B 8 28.40±3.84**△△ 33.86±3.63**△△ 189.31±35.80**△ 702.11±161.42**△△
Group C 8 6.92±2.43 42.56±2.73 109.40±16.09 123.05±63.25

Tab 2

Serum content of cytokines in each group on the fifth experiment day ($\bar{x}$±s)"

Group N TNF-α (ng/L) IL-2 (ng/L) IL-6 (μg/L) IL-8 (μg/L)
Group A 8 48.63±10.52** 25.94±4.01** 184.10±60.91** 982.80±149.56**
Group B 8 29.70±3.51**△△ 32.87±4.08**△△ 166.70±66.61** 820.10±173.91**△
Group C 8 5.76±2.20 45.76±3.45 77.40±9.06 117.10±44.64

Tab 3

Serum content of inflammatory mediators in each group on the third experiment day ($\bar{x}$±s)"

Group n NO (μmol/L) CRP (mg/L)
Group A 6 130.10±30.85** 1 520.0±217.0**
Group B 6 98.40±16.38**△ 1 208.0±242.0**△
Group C 6 16.00±6.31 2.0±0.3

Tab 4

Serum content of inflammatory mediators in each group on the fifth experiment day ($\bar{x}$±s)"

Group n NO (μmol/L) CRP (mg/L)
Group A 6 232.50±87.42** 1 309.0±171.0**
Group B 6 180.20±85.76** 933.0±167.0**△△
Group C 7 17.70±5.16 2.0±0.4

Tab 5

Serum content of endotoxin in each group on the third experiment day ($\bar{x}$±s, EU/ml)"

Group n Endotoxin
Group A 10 0.41±0.14
Group B 10 0.21±0.08**
Group C 10 0.08±0.03

Tab 6

Numbers of translocated bacteria in liver, spleen and mesenteric lymph nodes in each group on the third experiment day ($\bar{x}$±s, cfu/0.1 ml)"

Group n Liver Spleen MLN
Group A 10 61.60±4.45** 28.20±6.16** 36.80±8.92**
Group B 10 13.80±8.92**△△ 14.20±2.10**△△ 16.70±1.77**△△
Group C 10 2.40±1.84 2.30±2.00 4.20±2.82
[1] 高炬, 张静喆, 朱培庭 . 锦红片治疗急性胆道感染的临床研究[J]. 上海中医药大学学报, 2002,16(3):42-43
[2] 龚建平, 韩本立 . 急性胆道感染时肠粘膜屏障损伤的研究[J]. 中华实验外科杂志, 1991,8(3):115-116
[3] 王伟军 . 肠道细菌移位与外科临床的联系[J]. 国外医学创伤与外科基本问题分册, 1994,15(3):135-139
[4] 曹建西, 侯智亮, 彭红光 . 生大黄配合抗生素治疗急性重症胆管炎24例[J]. 中医杂志, 2004,45(2):127-128
doi: 10.3321/j.issn:1001-1668.2004.02.032
[5] 胡家石, 吴咸中 . 内镜鼻胆管引流-清解灵治疗急性重型胆管炎的临床观察[J]. 中西医结合杂志, 1989,9(3):144-147
[6] 天津市南开医院. 遵义医学院主编.新急腹症学(第1版)[M]. 北京: 人民卫生出版社, 1978,( 第1版):416-423
[7] Fong Y, Moldawer LL, Shires GT , et al. The biologic characteristics of cytokines and their implication in surgical injury[J]. Surg Gynecol Obstet, 1990,170(4):363-378
doi: 10.1002/pd.1970100414 pmid: 2108507
[8] 梁华平 . 创伤后白介素-2产生受抑的机制及其研究进展[J]. 国外医学·军事医学分册, 1991,8(4):145-149
[1] Wen Sun, Jian Yu, Yu-min Shi, Hao Zhang, Ying Wang, Bing-bing Wu. Effects of Cordyceps extract on cytokines and transcription factors in peripheral blood mononuclear cells of asthmatic children during remission stage. Journal of Chinese Integrative Medicine, 2010, 8(4): 341-346.
[2] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013.
[3] Ju-yong Wang, Zhan Zheng, Zhen-ye Xu, Jing Guo, Wan So. Effects of Feiyanning Decoction on proliferation of lung adenocarcinoma cell line and their production of interleukin-6 and interleukin-8 induced by tumor necrosis factor-α. Journal of Chinese Integrative Medicine, 2009, 7(8): 741-745.
[4] Wei Zhang, Jing-tao Cao, Hai-yu Liu. Effects of pathogenic wind-dampness on lung tissue cytokines in rats with syndrome due to pathogenic cold invading lung. Journal of Chinese Integrative Medicine, 2008, 6(7): 748-751.
[5] Sheng-li Tian, Yong-ming Zhou, Tao Huang, Zhi-zhong Xue, Wei He. Effects of Yisui Jiedu Recipe on JAK2-STAT5 signal transduction pathway in bone marrow hematopoietic cells from patients with myelodysplastic syndrome-refractory anemia. Journal of Chinese Integrative Medicine, 2008, 6(2): 185-189.
[6] Xin-bin Sui, Qing Zhang, Hua-sen Qiu, Ji-chun Zhou, Xi-dong Gu, Zhou-xiang Lu, Chao Li, Gang Wang. Mechanism of salvianolate in preventing postoperative intestinal adhesion in rats. Journal of Chinese Integrative Medicine, 2007, 5(5): 521-525.
[7] Jing-zhe Zhang, Xue-lin Zhang, Ju Gao, Ping Shen, Pei-ting Zhu. Impact of Jinhong Tablet on thymus of rats with cholangitis. Journal of Chinese Integrative Medicine, 2007, 5(2): 174-178.
[8] Guo-lin Peng, Xiao-yun Zhang. Effects of Salvia miltiorrhiza on serum levels of inflammatory cytokines in patients with severe acute pancreatitis. Journal of Chinese Integrative Medicine, 2007, 5(1): 28-31.
[9] Jin-shui Zhu, Ming-quan Song, Long Wang, Qun Sun, Li Zhu, Chun Fang. Immunoregulation and short-term therapeutic effects of super-selective intra-arterial chemotherapy combined with traditional Chinese drugs on gastric cancer patients. Journal of Chinese Integrative Medicine, 2006, 4(5): 478-481.
[10] Qiang Wang​, Kun-zheng Wang​, Guang-ye Wang​. Effects of soybean isoflavones on expression levels of osteoprotegerin and osteoprotegerin ligand mRNAs in bone tissues of ovariectomized rats. Journal of Chinese Integrative Medicine, 2006, 4(3): 303-306.
[11] Yong-qing Cao, Chun-mei He, Jin-gen Lu. Effects of Wenshen Jianpi Recipe on chronic wound healing in rats. Journal of Chinese Integrative Medicine, 2005, 3(3): 220-223.
[12] Jun Cai, Zheng-rong Xuan, Yong-ping Wei, Hai-bo Yang, Hua Wang. Effects of perioperative administration of rhubarb on acute inflammatory response in patients with gastric cancer. Journal of Chinese Integrative Medicine, 2005, 3(3): 195-198.
[13] Guang Ji. Clinical study on treatment of alcoholic liver disease by Qinggan Huoxue Recipe. Journal of Chinese Integrative Medicine, 2003, 1(2): 103-107.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
[an error occurred while processing this directive]