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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (2): 156-159.doi: 10.3736/jcim20060210

• Original Experimental Research • Previous Articles     Next Articles

Effects of ginsenosides extracted from ginseng stem and leaves on glucocorticoid receptor in different viscera in heat-damaged rats

Min Li1, Chang-quan Ling2, Xue-qiang Huang2, Zhi-lei Shen1   

  1. 1. Department of Military Hygiene, Second Military Medical University, Shanghai 200433, China
    2. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Online:2006-03-20 Published:2006-03-15

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Objective

To evaluate the effects of ginsenosides (GSS) extracted from ginseng stem and leaves on glucocorticoid receptor (GR) in different viscera in heat-damaged rats, and to find out its action mechanism.

Methods

Thirty-two male SD rats were divided into control group and experimental group, and fed 2 mg/d GSS and equal-quantity of distilled water respectively for 7 days. Eight rats of each group were exposed to (42±1) ℃ for one hour. The binding activities of GR in brain, thymus, lung and liver cytosols in rats were detected by radioligand binding assay. The expression levels of GR mRNA in brain and liver cytosols were determined by reverse transcription-polymerase chain reaction (RT-PCR) assay. Plasma adrenocorticotropin (ACTH) and corticosterone (CS) concentrations were determined by radioimmunoassay. 

Results

The binding activities of GR in brain, lung and liver cytosols, and the expression levels of GR mRNA in brain and liver cytosols were all higher in the GSS-treated and heat-damaged rats than those in the untreated heat-damaged rats (P＜0.05 or P＜0.01). There were no significant differences in plasma concentrations of ACTH and CS between the GSS-treated heat-damaged rats and the untreated heat-damaged rats. 

Conclusion

GSS can lessen the descending degree of the binding activity of GR in brain, thymus, lung and liver cytosols, and such efficacy of GSS may be related to improvment of the expression of GR mRNA.

Key words: Ginsenosides, Ginseng stem and leaves, Glucocorticoid receptor, Heat damage, Rat

CLC Number: 

  • R285.5

Table 1

Effects of GSS on binding activities of GR in brain, thymus, lung and liver cytosols in heat-damaged rats (ヌ±S)"

GroupnBrain
(fmol/mg protein)		Thymus
(site/cell)		Lung
(fmol/mg protein)		Liver
(fmol/mg protein)
Normal control8165.03±30.464457.88±669.43404.98±87.16649.48±115.85
GSS-treated8166.86±29.124879.38±889.32402.79±82.74590.90±77.16
Heat-damaged810.25±3.53**3221.75±593.86*231.63±41.18**298.29±79.22**
GSS-treated and heat-damaged842.01±12.62**△△3975.43±898.39300.44±39.92**△465.95±88.95**△△

Figure 1

Effects of GSS on expression levels of GR mRNA in brain and liver cytosols in heat-damaged rats1-4: GR mRNA in cell sap of brain; 5-8: GR mRNA in cell sap of liver; 1 and 5: Normal control; 2 and 6: GSS-treated; 3 and 7: Untreated with heat damage; 4 and 8: GSS-treated with heat damage."

Table 2

Effects of GSS on expression levels of GR mRNA in brain and liver cytosols in heat-damaged rats (ヌ±S)"

GroupnGR mRNA/β-actin
Brain cytosolLiver cytosol
Normal control80.41±0.020.45±0.05
GSS-treated80.43±0.040.49±0.08
Heat-damaged80.21±0.03**0.27±0.04**
GSS-treated and heat-damaged80.34±0.07**△0.40±0.0.6△

Table 3

Effects of GSS on concentrations of plasma ACTH and CS in heat-damaged rats (ヌ±S)"

GroupnACTH (ng/L)CS (μg/L)
Normal control852.14±13.8139.32±24.60
GSS-treated861.11±14.5241.63±19.81
Heat-damaged8205.32±42.00**473.50±56.30**
GSS-treated and heat-damaged8210.23±52.64**448.12±44.25**
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