人参茎叶皂苷对热损伤大鼠不同脏器糖皮质激素受体的影响

doi:10.3736/jcim20060210

Abstract

Objective

To evaluate the effects of ginsenosides (GSS) extracted from ginseng stem and leaves on glucocorticoid receptor (GR) in different viscera in heat-damaged rats, and to find out its action mechanism.

Methods

Thirty-two male SD rats were divided into control group and experimental group, and fed 2 mg/d GSS and equal-quantity of distilled water respectively for 7 days. Eight rats of each group were exposed to (42±1) ℃ for one hour. The binding activities of GR in brain, thymus, lung and liver cytosols in rats were detected by radioligand binding assay. The expression levels of GR mRNA in brain and liver cytosols were determined by reverse transcription-polymerase chain reaction (RT-PCR) assay. Plasma adrenocorticotropin (ACTH) and corticosterone (CS) concentrations were determined by radioimmunoassay.

Results

The binding activities of GR in brain, lung and liver cytosols, and the expression levels of GR mRNA in brain and liver cytosols were all higher in the GSS-treated and heat-damaged rats than those in the untreated heat-damaged rats (P＜0.05 or P＜0.01). There were no significant differences in plasma concentrations of ACTH and CS between the GSS-treated heat-damaged rats and the untreated heat-damaged rats.

Conclusion

GSS can lessen the descending degree of the binding activity of GR in brain, thymus, lung and liver cytosols, and such efficacy of GSS may be related to improvment of the expression of GR mRNA.

Key words: Ginsenosides, Ginseng stem and leaves, Glucocorticoid receptor, Heat damage, Rat

CLC Number:

R285.5

Min Li, Chang-quan Ling, Xue-qiang Huang, Zhi-lei Shen. Effects of ginsenosides extracted from ginseng stem and leaves on glucocorticoid receptor in different viscera in heat-damaged rats. Journal of Chinese Integrative Medicine, 2006, 4(2): 156-159.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20060210

http://www.jcimjournal.com/EN/Y2006/V4/I2/156

TrendMD

Figures/Tables 4

Table 1

Figure 1

Table 2

Table 3

References 6

[1]	李敏, 凌昌全, 沈志雷, 等.人参茎叶皂苷对热损伤大鼠糖皮质激素受体的影响[J].第二军医大学学报,2003,24(8):S16-S17 doi: 10.3321/j.issn:0258-879X.2003.08.054
[2]	徐仁宝, 卢健, 蒋左庶.受体的基础与临床(第1版)[M].上海: 上海科学技术出版社,1992,(第1版):100-135
[3]	Filipovic D, Gavrilovic L, Dronjak S, et al.Brain glucocorticoid receptor and heat shock protein 70 levels in rats exposed to acute, chronic or combined stress[J].Neuropsychobiology,2005,51(2):107-114
[4]	徐仁宝. 糖皮质激素受体减少在休克发生中的作用:一个新假说[J].生命科学,1997,9(2):78-80
[5]	Li D, Sancher ER.Glucocorticoid receptor and heat shock factor 1: novel mechanism of reciprocal regulation[J].Vitam Horm,2005,71():239-262
[6]	李敏, 凌昌全, 沈志雷, 等.人参茎叶皂甙提高机体热耐受能力的作用[J].中国公共卫生,2003,19(12):1473-1474 doi: 10.11847/zgggws2003-19-12-40

Related Articles 15

[1]	Tara Rajendran. Addressing the need for personalizing music therapy in integrative oncology. Journal of Integrative Medicine, 2022, 20(4): 281-283.
[2]	Yen-Lin Chao, Yi-Ai Rau, Hong-Sheng Shiue, Jiun-Lin Yan, Yuan-Yun Tang, Shao-Wen Yu, Bo-Yan Yeh, Yen-Lung Chen, Tsung-Hsien Yang, Shu-Chen Cheng, Yi-Wen Hsieh, Hsin-Chia Huang, Fu-Kuang Tsai, Yu-Sheng Chen, Geng-Hao Liu. Using a consensus acupoints regimen to explore the relationship between acupuncture sensation and lumbar spinal postoperative analgesia: A retrospective analysis of prospective clinical cooperation. Journal of Integrative Medicine, 2022, 20(4): 329-337.
[3]	Lu Yin, Yun An, Xiao Chen, Hui-xin Yan, Tao Zhang, Xin-gang Lu, Jun-tao Yan. Local vibration therapy promotes the recovery of nerve function in rats with sciatic nerve injury. Journal of Integrative Medicine, 2022, 20(3): 265-273.
[4]	Marisa Casal. Improving the health and treatment success rates of in vitro fertilization patients with traditional chinese medicine: need for more robust evidence and innovative approaches. Journal of Integrative Medicine, 2022, 20(3): 187-192.
[5]	Miriam Bazzicalupo, Laura Cornara, Bruno Burlando, Alberta Cascini, Marcella Denaro, Antonella Smeriglio, Domenico Trombetta. Carpobrotus edulis (L.) N.E.Br. extract as a skin preserving agent: from traditional medicine to scientific validation. Journal of Integrative Medicine, 2021, 19(6): 526-536.
[6]	Xin Yin, Shu-bin Cai, Lan-ting Tao, Lu-ming Chen, Zhong-de Zhang, Su-hong Xiao, Arthur Yin Fan, Xu Zou. Recovery of a patient with severe COVID-19 by acupuncture and Chinese herbal medicine adjuvant to standard care. Journal of Integrative Medicine, 2021, 19(5): 460-466.
[7]	Rui-jin Qiu, Min Li, Jia-yuan Hu, Jing Chen, Hong-cai Shang. Methods for development of a core outcome set for clinical trials integrating traditional Chinese medicine and Western medicine. Journal of Integrative Medicine, 2021, 19(5): 389-394.
[8]	Reem Abdul-Latif, Ieva Stupans, Ayman Allahham, Benu Adhikari, Thilini Thrimawithana. Natural antioxidants in the management of Parkinson’s disease: review of evidence from cell line and animal models. Journal of Integrative Medicine, 2021, 19(4): 300-310.
[9]	Fidele Ntchapda, Fernand C. Tchatchouang, David Miaffo, Barthelemy Maidadi, Lorella Vecchio, Rodrigue E. Talla, Christian Bonabe, Paul F. Seke Etet, Theophile Dimo. Hypolipidemic and anti-atherosclerogenic effects of aqueous extract of Ipomoea batatas leaves in diet-induced hypercholesterolemic rats. Journal of Integrative Medicine, 2021, 19(4): 243-250.
[10]	Sibel Şentürk, Dilek Efe Arslan, Adil Çetinkaya. Association of complementary and integrative therapy use and symptoms among Turkish patients with familial Mediterranean fever. Journal of Integrative Medicine, 2021, 19(4): 340-346.
[11]	Tian Fang, Ze-sheng Xue, Jia-xuan Li, Jia-kuan Liu, Di Wu, Mei-qian Li, Yu-ting Song, Shi-feng Yun, Jun Yan. Rauwolfia vomitoria extract suppresses benign prostatic hyperplasia by reducing expression of androgen receptor and 5α-reductase in a rat model. Journal of Integrative Medicine, 2021, 19(3): 258-264.
[12]	Wen-jing Li, Chao Gao, Li-xin An, Yu-wei Ji, Fu-shan Xue, Yi Du. Perioperative transcutaneous electrical acupoint stimulation for improving postoperative gastrointestinal function: A randomized controlled trial. Journal of Integrative Medicine, 2021, 19(3): 211-218.
[13]	Mohamed Ismail Nounou, Heba A. Eassa, Nada A. Helal, Khaled Aboul Fotouh, Ihab Mansoor, Isabel K. Latz, Cindy Zheng, Hadeer A. Eassa, Dina Mohamed, Diana M. Huynh, Abigail R. Wiss, Jessica Sweeney, Monica T. Oakes, Mark M. Mikhail, Nadine Amine, Harshvir Kaur, Natalia Echeverry, Kamila Orzechowski, Doreen Szollosi. The safety, efficacy and pharmaceutical quality of male enhancement nutraceuticals bought online: Truth versus claim. Journal of Integrative Medicine, 2021, 19(3): 232-242.
[14]	Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[15]	Hua Zhang, Xiang-yang Yao, Dan-feng Zhang, Qi-le Guo, Jun-jun Yang, Bao-guo Zhu, Dong-xiao Shen, Ke-feng Ruan, Yuan Wang, Jun-liang Peng. Anti-hemorrhoidal activity of Lian-Zhi-San, a traditional Chinese medicine, in an experimental hemorrhoidal model in rats. Journal of Integrative Medicine, 2021, 19(1): 42-49.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[2]	Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3]	Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4]	Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[5]	A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H₂₂ cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[6]	Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[7]	Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8]	Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9]	Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017
[10]	Bo Wang , Wei Yan , Li-hui Hou, Xiao-ke Wu. Disorder of Tiangui (kidney essence) and reproductive dysfunction in patients with polycystic ovary syndrome. Journal of Chinese Integrative Medicine, 2010, 8(11): 1018-1022

Group	n	Brain (fmol/mg protein)	Thymus (site/cell)	Lung (fmol/mg protein)	Liver (fmol/mg protein)
Normal control	8	165.03±30.46	4457.88±669.43	404.98±87.16	649.48±115.85
GSS-treated	8	166.86±29.12	4879.38±889.32	402.79±82.74	590.90±77.16
Heat-damaged	8	10.25±3.53**	3221.75±593.86*	231.63±41.18**	298.29±79.22**
GSS-treated and heat-damaged	8	42.01±12.62**^△△	3975.43±898.39	300.44±39.92**^△	465.95±88.95**^△△

Group	n	GR mRNA/β-actin
Group	n	Brain cytosol	Liver cytosol
Normal control	8	0.41±0.02	0.45±0.05
GSS-treated	8	0.43±0.04	0.49±0.08
Heat-damaged	8	0.21±0.03**	0.27±0.04**
GSS-treated and heat-damaged	8	0.34±0.07**^△	0.40±0.0.6^△

Group	n	ACTH (ng/L)	CS (μg/L)
Normal control	8	52.14±13.81	39.32±24.60
GSS-treated	8	61.11±14.52	41.63±19.81
Heat-damaged	8	205.32±42.00**	473.50±56.30**
GSS-treated and heat-damaged	8	210.23±52.64**	448.12±44.25**

Effects of ginsenosides extracted from ginseng stem and leaves on glucocorticoid receptor in different viscera in heat-damaged rats

RichHTML

PDF (PC)

Cited

Like