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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (5): 495-499.doi: 10.3736/jcim20060511

• Original Experimental Research • Previous Articles     Next Articles

Effects of Jianpi Yishen Recipe on recurrence, metastasis and life span of mice transplanted with proventriculus squamous carcinoma after tumorectomy

Jie Wu1,Yu-qin Liu2,Ji-hong Dong2,Xiao-po Tang1,Jie Li1,Gui-zhi Sun1   

  1. 1. Department of Oncology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China
    2. Institute of Basic Medicine, Beijing Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, China
  • Online:2006-09-30 Published:2006-09-15

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Objective

To observe the effects of Jianpi Yishen Recipe (JPYSR), a compound Chinese herbal medicine, on recurrence, metastasis and life span of mice transplanted with proventriculus squamous carcinoma cells after tumorectomy.

Methods

JPYSR was orally administered to 615 mice transplanted with proventriculus squamous carcinoma cells in the palma of left hind limb after tumorectomy. The local tumor recurrence, lung metastasis and life span of the mice were evaluated and compared with those of the 5-FU-treated mice and untreated mice.

Results

The pulmonary metastasis rate was 94.4 % and the recurrence rate was 94.44 % in untreated group. The pulmonary metastasis rate was 68.4%, the inhibition rate of pulmonary metastasis was 27.5%, the recurrence rate was 78.95%, and the inhibition rate of tumor recurrence was 65.35% in JPYSR-treated group. The average and median life spans were obviously prolonged in JPYSR-treated group, as compared with those in untreated group. The life-prolonging rate was 100%.

Conclusion

JPYSR can effectively inhibit the local recurrence and pulmonary metastasis of the transplanted proventriculus squamous carcinoma in mice after tumorectomy, and prolong the life span.

Key words: Stomach cancer, Neoplasm recurrence, Metastasis, Life span, Jianpi Yishen Recipe

CLC Number: 

  • R285.5

Table 1

Body weight and primary tumor weight of mice with proventriculus squamous carcinoma after tumorectomy（ヌ±S , g）"

Group n Body weight Weight of primary tumor
Untreated 20 21.90±1.94 0.32±0.07
5-FU-treated 20 21.94±2.09 0.31±0.06
JPYSR-treated 20 22.00±1.71 0.32±0.06

Figure 1

amples of resected palmae of left hind limb"

Table 2

Pulmonary metastasis in mice with proventriculus squamous carcinoma 15 days after tumorectomy"

Group n Number of mice in different grades of pulmonary metastasis Mean Rank Metastasis
rate (%)		 Inhibition rate
of metastasis (%)
0 Ⅰ Ⅱ Ⅲ
Untreated 18 1 3 5 9 37.33 94.4
5-FU-treated 19 5 5 5 4 25.84* 73.7 21.9
JPYSG-treated 19 6 5 6 2 22.79* 68.4 27.5

Figure 2

HE staining of pulmonary metastases in 4 groups (×40) A: Normal pulmonary tissue; B: Pulmonary metastases in untreated group; C: Pulmonary metastases in 5-FU-treated group; D: Pulmonary metastases in JPYSR-treated group."

Table 3

Tumor recurrence in mice with proventriculus squamous carcinoma 15 days after tumorectomy"

Group n Body weight (?x±s , g) Weight of recurrent
tumor (?x±s , g)		 Recurrence
rate (%)		 Inhibition rate
of recurrence (%)
Untreated 18 20.30±2.18 1.01±0.93 94.44
5-FU-treated 19 21.30±2.05 0.26±0.52** 68.42 74.26
JPYSG-treated 19 22.07±1.87* 0.35±0.35** 78.95 65.35

Table 4

Life span of mice with proventriculus squamous carcinoma"

Group n Survival time (d) Mean survival time Median survival
period		 Life-prolonging
rate (%)
Shortest Longest
Untreated 15 8 33 17 17
5-FU-treated 15 9 60 28 25 64.71
JPYSG-treated 15 12 60 34 29 100.00

Figure 3

Survival time (d) of mice with proventriculus squamous carcinoma"

Figure 4

Recurrence of transplanted proventriculus squamous carcinoma after tumorectomy"

Figure 5

Samples of pulmonary metastases of mice with proventriculus squamous carcinoma after tumorectomy"

Figure 6

A mouse with proventriculus squamous carcinoma 45 days after JPYSR treatment after tumorectomy"

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