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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (5): 512-516.doi: 10.3736/jcim20080516

• Original Experimental Research • Previous Articles     Next Articles

Effects of Dahuang Zhechong Pill on expressions of tissue inhibitor of metalloproteinase-1 and plasminogen activator inhibitor-1 in rats with glomerulosclerosis

Ji-hong Chen1, Wei Sun1, Dong Zhou1, Kun Gao1, Wei-ming He1, Li Liu2   

  1. 1. Department of Nephrology, National Medical Centre for Traditional Chinese Medicine Nephropathy, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China
    2. Molecular Laboratory, Department of Nephrology, Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China
  • Received:2008-03-14 Online:2008-05-20 Published:2008-05-15

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Objective: To explore the mechanisms of the beneficial effects of Dahuang Zhechong Pill (DHZCP), a Chinese patent herbal medicine, in treatment of chronic renal disease, and to investigate the effects of DHZCP on the expressions of renal tissue inhibitor of metalloproteinase-1 (TIMP-1) and plasminogen activator inhibitor-1 (PAI-1) mRNAs in rats with adriamycin-induced glomerulosclerosis.
Methods: Focal segmental glomerulosclerosis and diffuse mesangial proliferation were induced in rats by combined procedures, including unilateral nephrectomy, intravenous injection of adriamycin and giving high-fat foods. The rats were randomly divided into untreated group, benazepril-treated group and DHZCP-treated group. Another 6 rats were sham-operated as control group. After 12-week treatment, rats were sacrificed, reverse transcription polymerase chain reaction (RT-PCR) method and computerized image analytical technique were used to determine the expressions of TIMP-1 and PAI-1 mRNAs.
Results: Compared with the untreated group, the ratios of TIMP-1/β-actin and PAI-1/β-actin of the DHZCP-treated group were decreased, suggesting that DHZCP could down-regulate the expressions of TIMP-1 and PAI-1 mRNAs (P<0.05). Benazepril could significantly inhibit the expression of TIMP-1 mRNA compared with that of the untreated group (P<0.05).
Conclusion: DHZCP can down-regulate the expressions of TIMP-1 and PAI-1 mRNAs in renal tissues of rats with focal segmental glomerulosclerosis and diffuse mesangial proliferation, which may be its action mechanism in treating chronic renal disease.

Key words: Dahuang Zhechong Pill, glomerulosclerosis, focal segmental, tissue inhibitor of metalloproteinase- 1, plasminogen activator inhibitor-1, rats

CLC Number: 

  • R289.5

Figure 1

Pathological characteristics of renal tissue in rats in different groups (HE staining, ×200) A: Sham-operated group; B: Untreated group; C: Benazepril-treated group; D: DHZCP-treated group."

Figure 2

Expression of TIMP-1 mRNA in renal tissue in rats in different groups A: Sham-operated group; B: Untreated group; C: Benazepril-treated group; D: DHZCP-treated group."

Figure 3

Expression of PAI-1 mRNA in renal tissue in rats in different groups A: Sham-operated group; B: Untreated group; C: Benazepril-treated group; D: DHZCP-treated group."

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