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Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (9): 915-920.doi: 10.3736/jcim20080908

• Original Experimental Research • Previous Articles     Next Articles

Effects of Qufeng Tongluo Recipe on proliferation of and TGF-β1 and IL-6 mRNA expressions in glomerular mesangial cells from rats

Wan-sen Sun(), Zhu Wang, Juan Wang, Qiao-ya Ma, Xiao-qiang Zhang, Xi-li Wu   

  1. Department of Integrated Traditional Chinese and Western Medicine, Second Affiliated Hospital of College of Medicine,Xi’an Jiaotong University, Xi'an, Shaanxi Province 710004, China
  • Received:2008-01-29 Online:2008-09-20 Published:2008-09-15

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Objective: To investigate the effects of Qufeng Tongluo Recipe (QFTLR), a compound of traditional Chinese herbal medicine for dispelling wind and removing obstruction in the meridians, on cell proliferation and expressions of transforming growth factor-beta1 (TGF-β1) and interleukin-6 (IL-6) mRNAs induced by lippolysaccharide in glomerular mesangial cells from rats.
Methods: The method of serum pharmacology was used. A total of 32 rats were divided into normal control group, untreated group, QFTLR group and positive control group which also was named Monopril (fosinopril sodium) group. Mesangial proliferative glomerulonephritis was induced by injection of antithymocyte serum in the rats except for the normal control group. Sera of the rats were obtained after corresponding interventions. Lipopolysaccharide-induced proliferation of rat mesangial cells (MCs) cultured in the respective serum-containing media was detected by the method of methyl thiazolyl tetrazolium (MTT) assay, and the expressions of TGF-β1 and IL-6 mRNAs were analyzed by the method of reverse transcription polymerase chain reaction.
Results: Compared with the untreated group, QFTLR showed remarkable inhibitory function on the proliferation of the mesangial cells (P<0.05). The expressions of TGF-β1 mRNA in mesangial cells were increased in the untreated group, QFTLR group and Monopril group when compared with the normal control group (P<0.01), but the TGF-β1 mRNA expressions in QFTLR group and in Monopril group were lower than that in the untreated group. The IL-6 mRNA expression could be increased by the LPS stimulation, and it was significantly higher in the other three groups than that in the normal control group, including the untreated group, the Monopril group and the QFTLR group (P<0.01). Compared with the untreated group, the expression of IL-6 mRNA was decreased by QFTLR and Monopril (P<0.01). QFTLR was better than Monopril in inhibiting the proliferation of the mesangial cells and decreasing the expressions of TGF-β1 and IL-6 mRNAs (P<0.05).
Conclusion: QFTLR has great inhibitory effect on mesangial cell proliferation and expressions of TGF-β1 and IL-6 mRNAs, which may be one of its mechanisms in postponing glomerular sclerosis.

Key words: Qufeng Tongluo Recipe, mesangial cell, pharmacology (TCD), transforming growth factor-betal, interleukin-6, rats

CLC Number: 

  • R295.5

Table 1

Effects of QFTLR-containing serum on MC proliferation induced by LPS stimulation（x±s)"

Group n OD
Normal control 6 1.253±0.149
Untreated 6 1.960±0.205*
Monopril 6 1.712±0.121*
QFTLR 6 1.386±0.111△▲

Figure 1

Effects of QFTLR-containing serum on MC proliferation induced by LPS stimulation（Light microscopy, ×400） A: Normal control group; B: Untreated group; C: Monopril group; D: QFTLR group."

Table 2

Effects of QFTLR-containing serum on expression of TGF-β1 and IL-6 mRNAs in MC (x±s)"

Group n TGF-β1 mRNA/β-actin IL-6 mRNA/β-actin
Normal control 3 0.267±0.009 0.350±0.012
Untreated 3 0.740±0.012** 0.940±0.012**
Monopril 3 0.663±0.009**△△ 0.853±0.009**△△
QFTLR 3 0.443±0.062**△△▲▲ 0.627±0.018**△△▲▲

Figure 2

Expression of IL-6 mRNA in MC detected by reverse transcription polymerase chain reaction A: Normal control group; B: Untreated group; C: Monopril group; D: QFTLR group."

Figure 3

Expression of TGF-β1 mRNA in MC detected by reverse transcription polymerase chain reaction A: Normal control group; B: Untreated group; C: Monopril group; D: QFTLR group."

[1] Kashgarian M, Sterzel RB . The pathobiology of the mesangium[J]. Kidney Int, 1992,41(3):524-529
doi: 10.1038/ki.1992.74 pmid: 1573822
[2] Dong B, Zou WZ, You JF . A study on the proliferative and sclerosing mechanism of glomerular mesangium[J]. Zhonghua Bing Li Xue Za Zhi, 1994,23(1):10-13
doi: 10.1007/BF02007173
董葆, 邹万忠, 由江峰 . 肾小球系膜细胞增生与系膜硬化机制的初步探讨[J]. 中华病理学杂志, 1994,23(1):10-13
doi: 10.1007/BF02007173
[3] Liu JF, Wen JK, Yan X , et al.Model of rat with mesangial proliferative glomerulonephritis induced by antithymocyte serum[J].Zhongguo Bing Li Sheng Li Za Zhi, 1999, 15(3): 274, 277
刘静方, 温进坤, 严霞 , 等 .抗胸腺细胞血清法诱发大鼠系膜增殖性肾炎模型[J].中国病理生理杂志, 1999, 15(3): 274, 277
[4] Sun L, Yi ZW, Yu PL . Effect of tetramethylhyrazine on proliferation of human fetal mesangial cells[J]. Zhongguo Zhong Xi Yi Jie He Za Zhi, 1995,15(3):134-136
doi: 10.7661/CJIM.1995.3.134
孙林, 易著文, 虞佩兰 . 川芎嗪对人胎肾小球系膜细胞增殖的影响及其机理探讨[J]. 中国中西医结合杂志, 1995,15(3):134-136
doi: 10.7661/CJIM.1995.3.134
[5] Fu P, Liu F, Su S , et al. Signaling mechanism of renal fibrosis in unilateral ureteral obstructive kidney disease in ROCK1 knockout mice[J]. J Am Soc Nephrol, 2006,17(11):3105-3114
doi: 10.1681/ASN.2005121366
[6] Lu HZ, Zhou JH . Role of transforming growth factor-β on signal transduction and mesangial cell in glomerular fibrosis[J]. Shi Yong Er Ke Lin Chuang Za Zhi, 2007,22(17):1346-1348
卢宏柱, 周建华 . 转化生长因子-β作用于系膜细胞的信号转导与肾小球纤维化[J]. 实用儿科临床杂志, 2007,22(17):1346-1348
[7] Zhang J, Yu YH, Luo LX , et al. Effect of Yushen Granule on expression of TGF-β in mesangial cells in rats with angiotensin Ⅱ irritating[J]. Zhongguo Zhong Xi Yi Jie He Shen Bing Za Zhi, 2005,6(6):354-356
张君, 于英华, 罗立欣 , 等. 愈肾颗粒对血管紧张素Ⅱ刺激大鼠肾系膜细胞TGF-β表达的影响[J]. 中国中西医结合肾病杂志, 2005,6(6):354-356
[8] Ruef C, Budde K, Lacy J , et al. Interleukin 6 is an autocrine growth factor for mesangial cells[J]. Kidney Int, 1990,38(2):249-257
doi: 10.1038/ki.1990.193 pmid: 2402117222
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