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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (1): 48-52.doi: 10.3736/jcim20090107

• Original Experimental Research • Previous Articles     Next Articles

Protective effects of n-butanol extract of Potentilla anserina on acute myocardial ischemic injury in mice

 Jian-yu Lia, Yi Lia, Hai-ying Gonga, Xiao-bo Zhaob, Ling-zhi Lia   

  1. a Division of Pharmaceutical Chemistry, Department of Preventive Medicine, Medical College of Chinese People's Armed Police Forces, Tianjin 300162, China
    b Team 061 of Department of Pharmacy, Medical College of Chinese People's Armed Police Forces, Tianjin 300162, China
  • Received:2008-05-18 Accepted:2008-07-08 Online:2009-01-20 Published:2009-01-15

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Objective

To investigate the protective effects of the n-butanol extract of Potentilla anserina L. (NP) on pituitrin-induced acute myocardial ischemic injury in mice.
Methods

Ninety healthy female mice were randomly divided into normal control group, untreated group, Salvia miltiorrhiza group and low-, medium- and high-dose NP groups. Except for the normal control group, the mice were intraperitoneally injected with pituitrin (20 U/kg) to induce acute myocardial ischemic injury. Thirty minutes after induction, electrocardiogram was monitored, and height of the J spot was measured also. Activities of lactate dehydrogenase (LDH), creatine kinase (CK) and superoxide dismutase (SOD) and content of malondialdehyde (MDA) in serum of the mice were detected. The degree of myocardial ischemic injury in mice was observed by Nagar-Olsen staining.
Results

The moving up of J spots in the treated groups was significantly inhibited when comparing with the untreated group (P<0.01). Compared with untreated group, high- and medium-dose NP and Salvia miltiorrhiza could significantly decrease the activities of LDH, CK (P<0.01, P<0.05), increase the SOD activity (P<0.01) and decrease the content of MDA (P<0.05). However, no significant difference was observed between low-dose NP group and untreated group (P>0.05). Nagar-Olsen staining showed that high- and medium-dose NP and Salvia miltiorrhiza could significantly diminish the areas of cardiac muscles injured by ischemia, but low-dose NP had no effect on that.
Conclusion

NP has a remarkable protective effect on acute myocardial ischemic injury in mice.

Key words: Potentilla anserina L., Myocardial ischemia, Superoxide dismutase, Creatine kinase, Lactate dehydrogenase, Malondialdehyde, Mice

Table 1

Effects of NP on J spot change of electrocardiogram in mice with myocardial ischemic injury (x±s, mV)"

Group n J spot height
Normal control 15 0.020±0.022
Untreated 15 0.223±0.043**
Salvia miltiorrhiza 15 0.100±0.055**△△
Low-dose NP 15 0.145±0.016**△△
Medium-dose NP 15 0.111±0.034**△△
High-dose NP 15 0.105±0.038**△△

Table 2

Effects of NP on activities of serum LDH and CK in mice with myocardial ischemic injury (x±s, U/L)"

Group n LDH CK
Normal control 15 1 651±755 366±193
Untreated 15 5 769±827** 2 208±258**
Salvia miltiorrhiza 15 4 277±878**△△ 1 304±360**△△
Low-dose NP 15 5 848±788** 2 880±712**
Medium-dose NP 15 4 752±766**△△ 1 382±360**△
High-dose NP 15 2 650±917**△△ 715±340*△△

Table 3

Effects of NP on SOD activity and MDA content in serum of mice with myocardial ischemic injury (x±s)"

Group n SOD (kU/L) MDA (μmol/L)
Normal control 15 312.864±32.132 4.369±0.316
Untreated 15 208.781±33.425** 6.216±0.382**
Salvia miltiorrhiza 15 303.220±31.672△△ 5.405±0.382**△△
Low-dose NP 15 209.432±35.423** 6.081±0.506**
Medium-dose NP 15 244.920±37.423**△△ 5.586±0.221**△△
High-dose NP 15 288.621±43.346*△△ 4.595±0.483△△

Figure 1

Nagar-Olsen staining of cardiac muscles of mice in different groups (Light microscopy, ×200) A: Normal control group; B: Untreated group; C: Salvia miltiorrhiza group; D: Low-dose NP group; E: Medium-dose NP group; F: High-dose NP group."

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