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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (2): 125-129.doi: 10.3736/jcim20090206

• Original Experimental Research • Previous Articles     Next Articles

Effects of Feiyanning formula on expressions of mRNAs and proteins of CXCL12 and CXCR4 in mice with Lewis tumors

 Ji Wua, Bing Baia, Zhen-ye Xub, Zhong-qi Wanga, Xiao-zhen Zhaoa   

  1. a Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    b Cancer Institute of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2008-06-18 Accepted:2008-08-26 Online:2009-02-20 Published:2009-02-15

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Objective

To observe the effects of Feiyanning (FYN) formula, a compound traditional Chinese herbal medicine, on the expressions of CXCL12 and CXC chemokine receptor 4 (CXCR4) mRNAs and proteins in Lewis tumors in C57 mice.
Methods

A total of 24 C57BL-6 mice were used in this study. Lewis cells were subcutaneously injected to the right armpit to establish the tumor-bearing model. The C57 mice bearing Lewis tumor were randomly divided into untreated group, chemotherapy group, FYN group and chemotherapy plus FYN group. Reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were employed to measure the expressions of CXCL12 and CXCR4 mRNAs and proteins in Lewis tumors in C57 mice respectively.
Results

The rates of lung cancer metastasis in the FYN group and the chemotherapy plus FYN group were markedly lower than that in the untreated group or the chemotherapy group. The expressions of CXCL12 and CXCR4 mRNAs and proteins appeared in tumor tissue in the untreated group. Although there was no significant difference in the expressions of mRNA and protein of CXCL12 among all groups (P>0.05), the expressions of mRNA and protein of CXCR4 in the untreated group and the chemotherapy group were markedly lower than those in the FYN group and the chemotherapy plus FYN group (P<0.05).
Conclusion

FYN can inhibit the metastasis of Lewis tumor loaded in C57 mice. Its mechanisms may be related to its down-regulation of the expression of chemokine receptor CXCR4, and affecting the role of CXCL12-CXCR4 biological axis.

Key words: Feiyanning, Lewis lung carcinoma, CXCR4 receptors, CXC chemokines, Mice

Table 1

Primer sequence"

Gene Forward primer Reverse primer Size (bp)
GAPDH 5'-ACA GCC GCA TCT TCT TGT GCA GTG-3' 5'-GGC CTT GAC TGT GCC GTT GAA TTT-3' 174
CXCL12 5'-GTC AGC CTG AGC TAC CGA-3' 5'-GAA GGG CAC AGT TTG GAG-3' 103
CXCR4 5'-GGC TGA CCT CCT CTT TGT-3' 5'-GTT TCC TTG GCC TTT GAC-3' 203

Table 2

Expressions of CXCL12 and CXCR4 mRNAs in tumor tissues of mice in different groups (x±s)"

Group n Ratio of CXCL12
mRNA/GAPDH		 Ratio of CXCR4
mRNA/GAPDH
Untreated 6 0.97±0.37 1.41±0.67
Chemotherapy 6 1.31±0.58 1.34±0.47
FYN 6 1.21±0.54 0.29±0.17*△
FYN plus chemotherapy 6 1.23±0.42 0.35±0.18*△

Figure 1

PCR electropherogram of expressions of CXCL12 and CXCR4 mRNAs A: Untreated group; B: FYN group; C: FYN plus chemotherapy group; D: Chemotherapy group."

Table 3

Expressions of CXCL12 and CXCR4 proteins in tumor tissues of mice in different groups (x±s)"

Group n CXCL12 (×10–4) CXCR4(×10–2)
Untreated 6 6.07±3.11 5.01±2.45
Chemotherapy 6 5.98±3.04 4.60±2.37
FYN 6 5.98±3.23 2.30±0.99*△
FYN plus chemotherapy 6 5.94±2.34 2.50±0.82*△
[1] Shields PG, Harris CC . Cancer risk and low-penetrance susceptibility genes in gene-environment interactions[J]. J Clin Oncol, 2000,18(11):2309-2315
doi: 10.1200/JCO.2000.18.11.2309
[2] Xu ZY, Wang ZQ, Zhu YW, Zhou WD, Zhang H, Deng HB, Zhang M, Gao H, Jiang WJ . Influence of "Feiyanning Decoction" on late non-small cell lung cancer metastasis and serum vascular endothelial growth factor[J]. Shanghai Zhong Yi Yao Da Xue Xue Bao, 2003,17(3):18-22
徐振晔, 王中奇, 朱晏伟, 周卫东, 张晖, 邓海滨, 张铭, 高虹, 姜维洁 . 肺岩宁对晚期非小细胞肺癌生长转移和血清VEGF的影响[J]. 上海中医药大学学报, 2003,17(3):18-22
[3] Wang J, Ou ZL, Shao ZM . CXCL12-CXCR4 biological axis and tumor metastasis[J]. Guo Wai Yi Xue Zhong Liu Xue Fen Ce, 2004,31(5):326-330
王杰, 欧周罗, 邵志敏 . CXCR12-CXCL4生物学轴与肿瘤转移[J]. 国外医学肿瘤学分册, 2004,31(5):326-330
[4] Müller A, Homey B, Soto H, Ge N, Catron D, Buchanan ME, McClanahan T, Murphy E, Yuan W, Wagner SN, Barrera JL, Mohar A, Verastegui E, Zlotnik A .Involvement of chemokine receptors in breast cancer metastasis[J]. Nature, 2001,410(6824):50-56
doi: 10.1038/35065016
[5] Kato M, Kitayama S, Kazama S, Nagawa H . Expression pattern of CXC chemokine receptor-4 is correlated with lymph node metastasis in human invasive ductal carcinoma[J]. Breast Cancer Res, 2003,5(5):R144-R150
doi: 10.1186/bcr627
[6] Libura J, Drukala J, Majka M, Tomescu O, Navenot JM, Kucia M, Marquez L, Peiper SC, Barr FG, Janowska-Wieczorek A, Ratajczak MZ . CXCR4-SDF-1 signaling is active in rhabdomyosarcoma cells and regulates locomotion, chemotaxis, and adhesion[J]. Blood, 2002,100(7):2597-2606
doi: 10.1182/blood-2002-01-0031
[7] Gazitt Y . Homing and mobilization of hematopoietic stem cells and hematopoietic cancer cells are mirror image processes, utilizing similar signaling pathways and occurring concurrently: circulating cancer cells constitute an ideal target for concurrent treatment with chemotherapy and antilineage-specific antibodies[J]. Leukemia, 2004,18(1):1-10
doi: 10.1038/sj.leu.2403173
[8] Su LP, Zhang JP, Zheng XJ, Chu YW, Xiong SD . The effect of the expression level of CXCL4 on the metastatic potential of human lung cancer cells[J]. Zhongguo Zhong Liu Sheng Wu Zhi Liao Za Zhi, 2005,12(1):46-51
苏丽萍, 张进平, 郑秀娟, 储以薇, 熊思东 . CXCR4表达水平对肺癌细胞转移潜能的影响[J]. 中国肿瘤生物治疗杂志, 2005,12(1):46-51
[9] Jiang YP, Wu XH, Wu WX, Yin GR . Expressions of chemokine CXCL12 and its receptor CXCR4 and the clinical significance in human epithelial ovarian cancer[J]. Zhong Liu, 2006,26(9):851-855
蒋玉萍, 吴小华, 吴文新, 尹桂然 . CXCL12及受体CXCR4在卵巢上皮性癌中的表达及其临床意义[J]. 肿瘤, 2006,26(9):851-855
[10] Ma XT, Yu LW, Wang S, Du RY, Cui ZR . Relationship between the CXC chemokine receptor-4/CXCL12 signal transduction pathway and lymph node metastasis of gastric carcinoma[J]. Zhongguo Zhong Liu Lin Chuang, 2007,34(10):544-546, 549
马向涛, 余力伟, 王杉, 杜如昱, 崔志荣 . 趋化因子受体CXCR4/CXCL12信号转导通路与胃癌淋巴结转移关系的研究[J]. 中国肿瘤临床, 2007,34(10):544-546, 549
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