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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (2): 145-150.doi: 10.3736/jcim20090210

• Original Experimental Research • Previous Articles     Next Articles

Salvianolic acid B in vitro inhibited matrix metalloproteinases-1, -2, and -9 activities

 Ya-hui Lianga, Ping Lib, Qi-fu Huanga, Jing-xia Zhaob, Xin Liub, Miao-ke Daib   

  1. a Department of Pathology, School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
    b Department of Pathophysiology, Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China
  • Received:2008-08-12 Accepted:2008-10-07 Online:2009-02-20 Published:2009-02-15

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Objective

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases, which as a group can degrade essentially all extracellular matrix components. The proteolytic property of the MMPs is important during wound healing to remove debris and facilitate cell migration. Targeting towards the decreased MMPs activities is a new treatment strategy for healing chronic wounds. Salvia miltiorrhiza is a popular Chinese herb that could promote chronic ulcers healing for topical use. Salvianolic acid B (Sal B) is the most abundant bioactive component in Salvia miltiorrhiza. The research was designed to explore the inhibitory effects of Sal B on MMP-1, MMP-2 and MMP-9 activities.
Methods

Pure human interstitial collagenase (MMP-1) or gelatinase A (MMP-2) was activated by p-aminophenylmercuric acetate (APMA), and was incubated with Sal B for 1 h. The activities were observed by quenched fluorescent substrate. Gelatinase B (MMP-9) is rich in polymorphonuclear neutrophils (PMN), so the rat PMN was used as a source of MMP-9 for MMPs activity assays. In vitro MMP-9 from rats' PMN lysate was incubated with Sal B for 1 h, and its activity was tested by gelatin zymography.
Results

Sal B dose-dependently inhibited the human MMP-1 and MMP-2 activities in the range of 0.002 4 to 0.3 g/L, with 50% inhibiting concentration (IC50) of (0.090±0.015) g/L and (0.080±0.005) g/L respectively. In the range of 0.003 to 0.3 g/L, Sal B could inhibit the MMP-9 activity (P<0.01).
Conclusion

The broad-spectrum inhibitory effects of Sal B on MMPs may reveal one of the mechanisms for the effects of Salvia miltiorrhiza on chronic wounds.

Key words: Salvia miltiorrhiza, Salviol, Matrix metalloproteinases, Rats

Figure 1

Effects of Sal B on the activity of MMP-1"

Figure 2

Effects of Sal B on the activity of MMP-2"

Figure 3

Inhibition ratios of different concentrations of Sal B on the activity of MMP-1"

Figure 4

Inhibition ratios of different concentrations of Sal B on the activity of MMP-2"

"

Group n Inhibition ratio of MMP-1 Inhibition ratio of MMP-2
Control 3 0 0
0.002 4 g/L Sal B 3 33.20±6.56** 34.17±3.15**
0.012 g/L Sal B 3 47.23±3.29** 43.97±2.67**
0.06 g /L Sal B 3 61.83±4.70** 71.27±6.21**
0.3 g /L Sal B 3 83.03±15.67** 90.83±3.09**

Figure 5

The lysis bands of MMP-9 1: Marker; 2, 3: 0 g/L Sal B; 4, 5: 0.003 g/L Sal B; 6, 7: 0.03 g/L Sal B; 8, 9: 0.3 g/L Sal B; 10, 11: 1, 10-phenanthroline."

"

Group n Relative activity of MMP-9
Control 4 1.124 2±0.145 9
0.003 g/L Sal B 4 0.862 3±0.189 2**
0.03 g/L Sal B 4 0.516 0±0.151 6**
0.3 g/L Sal B 4 0.283 9±0.125 3**
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