Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (2): 151-156.doi: 10.3736/jcim20090211

• Original Experimental Research • Previous Articles     Next Articles

Effects of Salviae miltiorrhizae and salvianolic acid B on microcirculation of liver in mice with portal hypertension

 Tian Tian,  Lie-ming Xu   

  1. Institute of Liver Disease, Key Laboratory of Liver and Kidney Disease of the Ministry of Education, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Received:2008-08-11 Accepted:2008-10-25 Online:2009-02-20 Published:2009-02-15

Objective

To observe the effects of Salviae miltiorrhizae and its component, salvianolic acid B (SA-B), on the microcirculation of liver in mice with portal hypertension induced by endothelin-1 (ET-1).
Methods

Eighty-four Kunming mice were randomly divided into 7 groups: untreated group, endothelin A receptor (ETAR) blocker group, Astragali mongolici group, Astragalus polysaccharides (APS) group, Corydalis Yanhusuogroup, Salviae miltiorrhizae group and SA-B group. There were 12 mice in each group. Mice were pretreated with a corresponding equivalent volume of drug or distilled water for 3 days, and then the portal hypertension in mice was induced by continuous injection of ET-1 into coccygeal vein using a micro-injection pump. Six mice in each group were used to observe the average liver blood flow volume by laser-Doppler flow instrument before and after injection of ET-1, and the other six rats were used to observe the hepatic microcirculation velocity in vivo by an inverted microscope.
Results

The average blood flow of liver in mice decreased in each group after ET-1 injection. But the changes of average blood flow in the SA-B group and the ETAR blocker group were less than that in the untreated group (P<0.01). The changes of average blood volume in the Astragali mongolici group and the APS group were similar to that in the untreated group, but more than that in the SA-B group after injection of ET-1. The change of average blood flow in the SA-B group showed no significant difference when compared with the ETAR blocker group. The microcirculatory flow velocity in liver also decreased in each group after ET-1 injection. But the changes of microcirculatory flow velocity in the SA-B group and the ETAR blocker group were less than that in the untreated group (P<0.05, P<0.01). There were no significant differences in the changes of microcirculatory flow velocity among the Salviae miltiorrhizae group, the SA-B group and the ETAR blocker group.
Conclusion

Salviae miltiorrhizae and SA-B can decrease the average blood flow and microcirculatory flow velocity in liver in mice with portal hypertension, which may be one of the mechanisms of Salviae miltiorrhizae and SA-B in decreasing portal hypertension.

Key words: Liver cirrhosis, Hypertension, Portal, Microcirculation, Drugs, Chinese herbal, Mice

Figure 1

Picture of liver microcirculation video in mice (The green points are FITC-labeled red blood cells) A: Initial picture of liver microcirculation video; B: Sequence picture after being processed; C: FITC-RBC tracks on sequence picture after being processed. The lines in Figure C refer to the three tracks of the FITC-labeled red blood cells."

Table 1

Effects of different Chinese herbs and ETAR blocker on the lowered blood flow volume of liver in mice caused by ET-1(x±s, BPU)"

Group n Average blood flow volume
Before ET-1 injection After ET-1 injection Lowered range
Untreated 6 1 186.83±41.14 1 060.50±18.33 126.33±27.51
ETAR blocler 6 1 202.00±36.54 1 131.00±23.67 71.00±12.82△△
Corydalis yanhusuo 6 1 228.67±45.89 1 123.67±30.56 105.00±19.83▲▲
Astragali mongolici 6 1 197.17±52.29 1 068.83±27.17** 128.33±37.27▲▲□
Astragalus polysaccharides 6 1 189.83±49.72 1 069.33±40.44 120.50±24.20▲▲□
Salviae miltiorrhizae 6 1 275.50±40.60 1 175.83±31.50 99.67±15.82
SA-B 6 1 269.50±59.90 1 189.33±40.33 80.17±20.30△△

Figure 2

Average blood flow of each group before and after ET-1 injection A: Untreated group; B: ETAR blocker group; C: Corydalis yanhusuo group; D: Astragali mongolici group; E: Astragalus polysaccharides group; F: Salviae miltiorrhizae group; G: SA-B group. Separation line in each picture was used to separate before and after ET-1 injection."

Table 2

Effects of different Chinese herbs and ETAR blocker on the lowered liver microcirculatory flow velocity caused by ET-1(x±s, mm/s)"

Group n Liver microcirculation velocity
Before ET-1 injection After ET-1 injection Lowered range
Untreated 6 0.60±0.01 0.36±0.02** 0.23±0.02
ETAR blocler 6 0.60±0.05 0.43±0.01** 0.17±0.05△△
Corydalis yanhusuo 6 0.59±0.03 0.37±0.01** 0.22±0.04
Astragali mongolici 6 0.59±0.03 0.37±0.01** 0.23±0.02▲▲□
Astragalus polysaccharides 6 0.59±0.03 0.36±0.02** 0.23±0.03▲▲□
Salviae miltiorrhizae 6 0.63±0.02 0.38±0.01** 0.20±0.02
SA-B 6 0.62±0.04 0.39±0.01** 0.18±0.04
[1] He TL, Wu ZY . Current study of liver fibrosis with portal hypertension[J]. Zhonghua Wai Ke Gan Dan Za Zhi, 2006,12(4):287-288
何天霖, 吴志勇 . 门静脉高压症肝纤维化的研究现状[J]. 中华外科肝胆杂志, 2006,12(4):287-288
[2] Zhao JM, Zhou GD, Li WS, Wang SS, Sun YL, Yang JF, Pan D, Zhang LX, Guo SG, Niu JZ, Chen JM . An experimental study on the mechanism of anti-hepatic fibrosis with Chinese medicinal herbs Fufangbiejia-ruanganpian[J]. Jie Fang Jun Yi Xue Za Zhi, 2004,29(7):560-562
赵景民, 周光德, 李文淑, 王松山, 孙艳玲, 杨建法, 潘登, 张玲霞, 郭顺根, 牛建昭, 陈菊梅 . 复方鳖甲软肝片抗肝纤维化机制的实验研究[J]. 解放军医学杂志, 2004,29(7):560-562
[3] Iredale JP, Benyon RC, Pickering J, McCullen M, Northrop M, Pawley S, Hovell C, Arthur MJ .Mechanisms of spontaneous resolution of rat liver fibrosis. Hepatic stellate cell apoptosis and reduced hepatic expression of metalloproteinase inhibitors[J]. J Clin Invest, 1998,102(3):538-549
doi: 10.1172/JCI1018
[4] Zhou Y, Gu J, Xu LM . Effects of salvianolic acid B on elevated portal pressure and hepatic endothelin-1 level in rat model of hepatocirrhosis[J]. Zhongguo Zhong Xi Yi Jie He Xiao Hua Za Zhi, 2007,15(1):4-7
周扬, 顾杰, 徐列明 . 丹参酚酸B盐对肝硬化大鼠门静脉高压及肝组织中内皮素-1的影响[J]. 中国中西医结合消化杂志, 2007,15(1):4-7
[5] Chen HF .Microcirculation blood flow quantitative measurement system[M].Hangzhou: Master Thesis of Zhejiang University, 2002: 6
陈鸿峰 .微循环流速的定量测试系统[M].杭州: 浙江大学硕士学位论文, 2002: 6
[6] Chen SY . Current study on gene therapy for liver fibrosis[J]. Lin Chuang Gan Dan Bing Za Zhi, 2007,23(5):387-388
陈莎燕 . 肝纤维化的基因治疗现状[J]. 临床肝胆病杂志, 2007,23(5):387-388
[7] Liu XY . Current status of treatment of liver cirrhosis with portal hypertension[J]. Zhongguo Xian Dai Shou Shu Xue Za Zhi, 2003,7(1):15-17
刘浔阳 . 肝硬化门脉高压症研究治疗的现状[J]. 中国现代手术学杂志, 2003,7(1):15-17
[8] Sherman IA, Pappas SC, Fisher MM . Hepatic microvascular changes associated with development of liver fibrosis and cirrhosis[J]. Am J Physiol, 1990,258(2 Pt 2):H460-H465
[9] Leng XS . Prospects of basic researches concerning portal hypertension[J]. Wai Ke Li Lun Yu Shi Jian, 2002,7(4):258-260
冷希圣 . 门静脉高压症基础研究的近展与方向[J]. 外科理论与实践, 2002,7(4):258-260
[10] Niki T, Pekny M, Hellemans K, Bleser PD, Berg KV, Vaeyens F, Quartier E, Schuit F, Geerts A . Class Ⅵ intermediate filament protein nestin is induced during activation of rat hepatic stellate cells[J]. Hepatology, 1999,29(2):520-527
doi: 10.1002/(ISSN)1527-3350
[11] Ikura Y, Ohsawa M, Naruko T, Muraguchi T, Hirayama M, Suekane T, Fukushima H, Sugama Y, Shirai N, Kayo S, Yoshimi N, Ehara S, Tanzawa K, Ueda M . Expression of the hepatic endothelin system in human cirrhotic livers[J]. J Pathol, 2004,204(3):304-310
doi: 10.1002/(ISSN)1096-9896
[12] Tsai YT, Lin HC, Yang MC, Lee FY, Hou MC, Chen LS, Lee SD . Plasma endothelin levels in patients with cirrhosis and their relationships to the severity of cirrhosis and renal function[J]. J Hepatol, 1995,23(6):681-688
doi: 10.1016/0168-8278(95)80034-4
[13] Zhou Y, Gu J, Xu LM . Effect and mechanism of salvianolic acid B in attenuating elevated portal pressure in a rat model of portal hypertension induced by endothelin-1[J]. J Chin Integr Med, 2007,5(1):61-64
周扬, 顾杰, 徐列明 . 丹参酚酸B盐抑制内皮素-1所致大鼠门静脉压力升高的药效及其作用机制[J]. 中西医结合学报, 2007,5(1):61-64
[14] Liu P, Hu YY, Liu C, Xu LM, Liu CH, Sun KW, Huang DC, Yin YK, Zhou XQ, Wan MB, Cai X, Zhang ZQ, Ye J, Tang BZ, He J . Multicenter clinical study about the action of Fuzheng Huayu Capsule against liver fibrosis with chronic hepatitis B[J]. J Chin Integr Med, 2003,1(2):89-98, 102
刘平, 胡义扬, 刘成, 徐列明, 刘成海, 孙克伟, 胡德昌, 尹有宽, 周霞秋, 万谟彬, 蔡雄, 张志清, 叶军, 唐宝璋, 贺佳 . 扶正化瘀胶囊干预慢性乙型肝炎肝纤维化作用的多中心临床研究[J]. 中西医结合学报, 2003,1(2):89-98, 102
[15] Tao YY, Liu CH . Progress of research on mechanism of Salvia miltiorrhiza and its chemical ingredients against liver fibrosis[J]. J Chin Integr Med, 2004,2(2):145-148
陶艳艳, 刘成海 . 丹参及其化学成分抗肝纤维化作用机制研究进展[J]. 中西医结合学报, 2004,2(2):145-148
[1] Yi Ran Hu, San Li Xing, Chuan Chen, Ding Zhu Shen, Jiu Lin Chen. Tiaoxin Recipe, a Chinese herbal formula, inhibits microRNA-34a expression in the APPswe/PS1ΔE9 mouse model of Alzheimer's disease. Journal of Integrative Medicine, 2019, 17(6): 404-409.
[2] Pranay Soni, Rajesh Choudhary, Surendra H. Bodakhe. Effects of a novel isoflavonoid from the stem bark of Alstonia scholaris against fructose-induced experimental cataract. Journal of Integrative Medicine, 2019, 17(5): 374-382.
[3] Sonia Chew Wen Phang, Uma Devi Palanisamy, Khalid Abdul Kadir. Effects of geraniin (rambutan rind extract) on blood pressure and metabolic parameters in rats fed high-fat diet. Journal of Integrative Medicine, 2019, 17(2): 100-106.
[4] Bhesh Raj Sharma, Dong Wook Kim, Dong Young Rhyu. Korean Chungtaejeon tea extract attenuates weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2017, 15(1): 56-63.
[5] Nathalia Gomes Ribeiro de Moura, Ivan Cordovil, Arthurde Sá Ferreira. Traditional Chinese medicine wrist pulse-taking is associated with pulse waveform analysis and hemodynamics in hypertension. Journal of Integrative Medicine, 2016, 14(2): 100-113.
[6] Nouran A. Aleyeidi, Khaled S. Aseri, Shadia M. Matbouli, Albaraa A. Sulaiamani, Sumayyah A. Kobeisy. Effects of wet-cupping on blood pressure in hypertensive patients: A randomized controlled trial. Journal of Integrative Medicine, 2015, 13(6): 391-399.
[7] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[8] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[9] Ya Yuwen, Yu-qi Liu, Yan-ping Wang, Jin-gang Dai, Da-sheng Liu, Yue-xi Wang, Xue-jie Han. The add-on effect of a Chinese herbal formula for patients with resistant hypertension: Study protocol for a pilot cohort study. Journal of Integrative Medicine, 2015, 13(2): 122-128.
[10] Vamsi Reddy, Arvind Sridhar, Roberto F. Machado, Jiwang Chen. High sodium causes hypertension: Evidence from clinical trials and animal experiments. Journal of Integrative Medicine, 2015, 13(1): 1-8.
[11] Yong-hong Lian, Mei-xia Fang, Li-guo Chen. Constructing protein-protein interaction network of hypertension with blood stasis syndrome via digital gene expression sequencing and database mining. Journal of Integrative Medicine, 2014, 12(6): 476-482.
[12] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[13] Chang-qing Zhao, Yang Zhou, Jian Ping, Lie-ming Xu. Traditional Chinese medicine for treatment of liver diseases: Progress, challenges and opportunities. Journal of Integrative Medicine, 2014, 12(5): 401-408.
[14] Shu Dong, Shi-bing Su​. Advances in mesenchymal stem cells combined with traditional Chinese medicine therapy for liver fibrosis. Journal of Integrative Medicine, 2014, 12(3): 147-155.
[15] Md. Asadujjaman, Ahmed Ullah Mishuk, Md. Aslam Hossain, Utpal Kumar Karmakar. Medicinal potential of Passiflora foetida L. plant extracts: Biological and pharmacological activities. Journal of Integrative Medicine, 2014, 12(2): 121-126.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[5] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[6] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[7] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[8] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[9] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[10] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14