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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (3): 228-231.doi: 10.3736/jcim20090306

• Original Experimental Research • Previous Articles     Next Articles

Effects of cinobufacini injection on contents of serum thyroid-stimulating hormone and adrenaline in rats

 Shuang-shuang Wang, Xiao-feng Zhai , Bai Li   

  1. Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
  • Received:2008-09-01 Accepted:2008-11-24 Online:2009-03-20 Published:2009-03-15

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Objective

To observe the effects of cinobufacini injection on serum levels of thyroid-stimulating hormone (TSH) and adrenaline (ADR) in rats, and to speculate the property (cold or heat) of the drug.
Methods

Thirty-two male Wistar rats were randomly divided into normal control group, cinobufacini injection group, cold syndrome group and heat syndrome group. The rats in the four groups were administered with normal saline, cinobufacini injection, and compound of traditional Chinese drugs with heat or cold property. The weights, and the serum TSH and ADR levels of the rats were measured before and after the interventions.
Results

The weights of the rats in heat syndrome group markedly decreased after 14-day administration when compared with the normal control group (P<0.05). There were no significant differences among the normal control group, heat syndrome group and the cinobufacini injection group. Although no statistical difference was noted, the serum TSH levels of the cold syndrome group and cinobufacini injection group were slightly lower than that in the normal control group after 14-day administration, and the heat syndrome group showed a slightly higher serum TSH level. After administration, the serum ADR levels of the cold syndrome group and cinobufacini injection group were significantly lower than that in the normal control group (P<0.05), and the serum ADR level in the heat syndrom group was higher than that in the normal control group (P<0.05).
Conclusion

Cinobufacini injection seemed to be able to suppress the thyrotrophin-releasing hormone-TSH-thyroxine system and sympathetic nervous system, matching up with the characteristics of the Chinese herbs of cold property. And the study provided initial experiment evidence for the conclusion that the property of cinobufacini might be cold.

Key words: Cinobufacini injection, Cold (traditional Chinese drug), Heat (traditional Chinese drug), Rats

Table 1

Average body weights of rats in four groups before and after treatment（x±s, g）"

Group n Body weight
Before treatment After treatment
Normal 8 114.25±13.80 184.00±11.83
Heat syndrome 8 107.13±5.79 170.88±8.25*
Cold syndrome 8 107.25±6.63 175.75±10.39
Cinobufacini injection 8 116.88±10.90 183.00±3.59△

Table 2

Serum TSH content of rats in four groups before and after treatment（x±s, kU/L）"

Group n TSH
Before treatment After treatment
Normal 8 904.88±3.72 905.88±4.45
Heat syndrome 8 908.50±4.63 917.00±16.59
Cold syndrome 8 911.25±6.27 893.25±7.34
Cinobufacini injection 8 903.50±11.24 894.88±10.11

Table 3

Serum ADR content of rats in four groups before and after treatment（x±s, kU/L）"

Group n ADR
Before treatment After treatment
Normal 8 678.75±129.84 734.50±136.05
Heat syndrome 8 623.50±71.76 863.50±111.28*
Cold syndrome 8 739.00±95.82 591.38±80.77*△
Cinobufacini injection 8 640.63±110.98 578.38±132.32*△
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