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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (1): 74-79.doi: 10.3736/jcim20100114

• Original Experimental Research • Previous Articles     Next Articles

Effects of Xiaotan Sanjie Decoction on expressions of interleukin-8 and its receptors in gastric tumor xenografts and gastric tissue adjacent to the tumor in mice

 Da-wei Jua,Pin-kang Weia,Hui-ming Lina, Da-zhi Suna,Shan Yub,Li-juan Xiua   

  1. a Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
    b The 309th Hospital of Peopled Liberation Army, Beijing 100091, China
  • Received:2009-09-02 Accepted:2009-11-17 Online:2010-01-20 Published:2010-01-15
  • Contact: Pin-kang Wei E-mail:czzyk@smmu.edu.cn

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Objective

To explore the mechanisms of Xiaotan Sanjie Decoction (XTSJD), a compound traditional Chinese herbal medicine, in inhibiting the tumor growth and preventing recurrence by testing the protein expressions of interleukin-8 (IL-8) and its receptors chemokine receptor 1 (CXCR1) and chemokine receptor 2 (CXCR2) in gastric tumor xenografts and gastric tissue adjacent to the tumor in mice.
Methods

Fifty Kunming mice were randomly divided into normal group, normal saline (NS) group, Heat-clearing and Detoxicating Decoction (HCDD) group, tegafur (FT-207) group and XTSJD group. Except for mice in the normal group, S180 tumor block was transplanted into the gastric walls of the mice, and the mice were administered with corresponding medicine for 3 weeks. Weight of tumor xenografts was measured and tumor inhibition rate was calculated. IL-8 protein expression was tested by enzyme-linked immunosorbent assay. Expressions of CXCR1 and CXCR2 were tested by immunohistochemical method.
Results

The protein expressions of IL-8 and its receptors in tumor xenografts and gastric tissue adjacent to the tumor were markedly higher than those in the gastric tissue in normal mice (P<0.01); compared with HCDD and FT-207, XTSJD could significantly decrease the IL-8 protein expression in tumor xenografts and gastric tissue adjacent to the tumor (P<0.05); compared with FT-207, XTSJD could significantly decrease the CXCR1 protein expression in tumor xenografts (P<0.01), and XTSJD could also significantly decrease the CXCR1 protein expression in gastric tissue adjacent to the tumor as compared with HCDD and FT-207 (P<0.01); compared with HCDD and FT-207, XTSJD could significantly decrease the CXCR2 protein expression in tumor xenografts (P<0.01), and there was no significant difference among the three drug-treated groups in CXCR2 protein expression in gastric tissue adjacent to the tumor (P>0.05).
Conclusion

XTSJD can decrease the protein expressions of IL-8 and its receptors in tumor xenografts and gastric tissue adjacent to the tumor. It may be one of the mechanisms of XTSJD in inhibiting the tumor growth and preventing recurrence.

Key words: Xiaotan Sanjie Decoction, Gastric tumor, Interleukin-8, Chemokine receptor 1, Chemokine receptor 2, Mice

Table 1

Weight of gastric tumor xenografts and inhibition rate in different groups"

Group n Tumor weight ($\bar{x}±s$, g) Inhibition ate (%)
NS 10 2.43±0.36 –
HCDD 10 1.38±0.28△△▲▲ 43.21
FT-207 10 1.17±0.32△△ 51.85
XTSJD 10 1.01±0.21△△ 58.02

Table 2

Expression of IL-8 protein in gastric tumor xenografts and gastric tissue adjacent to tumor in different groups ($\bar{x}±s$, ng/L)"

Group n IL-8 protein
Tumor tissue Gastric tissue
adjacent to tumor
Normal 10 7.92±4.35
NS 10 47.22±4.84** 21.99±5.14**
HCDD 10 40.62±4.61**△△▲▲ 15.87±4.26**△△▲
FT-207 10 38.53±3.79**△△▲▲ 15.90±4.14**△△▲
XTSJD 10 29.42±4.38**△△ 11.03±4.85△△

Table 3

Expression of CXCR1 protein in gastric tumor xenografts and gastric tissue adjacent to tumor in different groups"

Group n Field distribution of expression
of CXCR2 protein in tumor tissue		 Field distribution of expression of CXCR2
protein in gastric tissue adjacent to tumor
– + ++ +++ P<0.01 – + ++ +++ P<0.01
Normal 100 95 4 1 0
NS 100 22 17 20 41 vs normal group 63 6 13 18 vs normal group
HCDD 100 52 15 13 20 vs normal and NS groups 72 8 12 8 vs normal, NS and XTSJD groups
FT-207 100 48 11 18 23 vs normal, NS and XTSJD roups 71 7 12 10 vs normal, NS and XTSJD groups
XTSJD 100 60 15 15 10 vs normal and NS groups 86 11 3 0 vs normal and NS groups

"

Group n Field distribution of expression
of CXCR2 protein in tumor tissue		 Field distribution of expression of CXCR2
protein in gastric tissue adjacent to tumor
– + ++ +++ P<0.01 – + ++ +++ P<0.01
Normal 100 91 7 2 0
NS 100 11 18 24 47 vs normal group 55 11 19 15 vs normal group
HCDD 100 31 11 27 31 vs normal, NS and XTSJD groups 71 10 12 7 vs normal and NS groups
FT-207 100 32 13 36 19 vs normal, NS and XTSJD groups 70 14 10 6 vs normal and NS groups
XTSJD 100 59 21 13 7 vs normal and NS groups 78 6 10 6 vs normal and NS groups
[1] Wei P, Xu L, Sun D, Shi J, Qin Z, Lu Y . Relations between phlegm and generation and development of gastric cancer[J]. J Tradit Chin Med, 2008,28(2):152-155
Wei PK, Yu ZH, Xu L, Qin ZF, Shi J, Sun DZ. Correlation between phlegm theory of traditional Chinese medicine and tumor stroma in gastric cancer[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi,2006,12(4):309-312 魏品康, 余志红, 许玲, 秦志丰, 施俊, 孙大志.中医胃癌痰证理论与细胞间质相关性探讨[J].中国中医基础医学杂志, 2006,12(4):309-312
[3] Chen JJ, Yao PL, Yuan A, Hong TM, Shun CT, Kuo ML, Lee YC, Yang PC . Up-regulation of tumor interleukin-8 expression by infiltrating macrophages: its correlation with tumor angiogenesis and patient survival in non-small cell lung cancer[J]. Clin Cancer Res, 2003,9(2):729-737
[4] Li XY, Wei PK . Clinic effects of Jinlongshe orally liquid in treating advanced carcinoma of stomach[J]. Hubei Zhong Yi Za Zhi, 2001,23(11):3-4
doi: 10.3969/j.issn.1000-0704.2001.11.001
李相勇, 魏品康 . 金龙蛇口服液治疗晚期胃癌的疗效观察[J]. 湖北中医杂志, 2001,23(11):3-4
doi: 10.3969/j.issn.1000-0704.2001.11.001
[5] Ju DW, Wei PK, Sun DZ, Lin HM, Yu S . Effect of Xiaotan Sanjie Decoction on the expression of IL-6 in mice with gastric transplanted tumor and gastric tissues beside tumor[J]. Zhongguo Zhong Xi Yi Jie He Xiao Hua Za Zhi, 2008,16(5):284-288
巨大维, 魏品康, 孙大志, 林晖明, 俞珊 . 消痰散结方对小鼠胃移植瘤及瘤旁胃组织中白细胞介素6表达的影响[J]. 中国中西医结合消化杂志, 2008,16(5):284-288
[6] Su LP, Zhang JP, Chen J, Wang Y, Xiong SD . Expression of CXCR4 in lung cancer and its significance[J]. Zhongguo Ai Zheng Za Zhi, 2005,15(2):97-100
doi: 10.3969/j.issn.1007-3639.2005.02.001
苏丽萍, 张进平, 陈晋, 王缨, 熊思东 . CXCR4在肺癌组织中的表达及临床意义[J]. 中国癌症杂志, 2005,15(2):97-100
doi: 10.3969/j.issn.1007-3639.2005.02.001
[7] Kitadai Y, Haruma K, Sumii K, Yamamoto S, Ue T, Yokozaki H, Yasui W, Ohmoto Y, Kajiyama G, Fidler IJ, Tahara E . Expression of interleukin-8 correlates with vascularity in human gastric carcinomas[J]. Am J Pathol, 1998,152(1):93-100
doi: 10.1097/00002093-199803001-00006 pmid: 9422527
[8] Macrì A, Versaci A, Loddo S, Scuderi G, Travagliante M, Trimarchi G, Teti D, Famulari C . Serum levels of interleukin 1beta, interleukin 8 and tumour necrosis factor alpha as markers of gastric cancer[J]. Biomarkers, 2006,11(2):184-193
doi: 10.1080/13547500600565677
[9] Davidson B, Reich R, Kopolovic J, Berner A, Nesland JM, Kristensen GB, Tropé CG, Bryne M , Risberg B, van de Putte G, Goldberg I.Interleukin-8 and vascular endothelial growth factor mRNA and protein levels are down-regulated in ovarian carcinoma cells in serous effusions[J]. Clin Exp Metastasis, 2002,19(2):135-144
doi: 10.1023/A:1014582911680
[10] Konno H, Ohta M, Baba M, Suzuki S, Nakamura S . The role of circulating IL-8 and VEGF protein in the progression of gastric cancer[J]. Cancer Sci, 2003,94(8):735-740
doi: 10.1111/j.1349-7006.2003.tb01511.x pmid: 12901801
[11] Fu H, Yuan Y, Sun XJ, Wang L, Ma MC, Li FC, Zhao YY, Wang JM, Sun KL . The expression of IL-8 gene in precancerous condition and gastric cancer[J]. Zhongguo Zhong Liu Lin Chuang, 2002,29(4):237-239
doi: 10.3969/j.issn.1000-8179.2002.04.003
付浩, 袁媛, 孙秀菊, 王兰, 马明超, 李福才, 赵彦艳, 王吉民, 孙开来 . IL-8基因在胃癌及癌前状态中的表达[J]. 中国肿瘤临床, 2002,29(4):237-239
doi: 10.3969/j.issn.1000-8179.2002.04.003
[12] Kitadai Y, Takahashi Y, Haruma K, Naka K, Sumii K, Yokozaki H, Yasui W, Mukaida N, Ohmoto Y, Kajiyama G, Fidler IJ, Tahara E . Transfection of interleukin-8 increases angiogenesis and tumorigenesis of human gastric carcinoma cells in nude mice[J]. Br J cancer, 1999,81(4):647-653
doi: 10.1038/sj.bjc.6690742
[13] Kitadai Y, Haruma K, Mukaida N, Ohmoto Y, Matsutani N, Yasui W, Yamamoto S, Sumii K, Kajiyama G, Fidler IJ, Tahara E . Regulation of disease-progression genes in human gastric carcinoma cells by interleukin 8[J]. Clin Cancer Res, 2000,6(7):2735-2740
[14] Bizzarri C, Beccari AR, Bertini R, Cavicchia MR, Giorgini S, Allegretti M . ELR+CXC chemokines and their receptors(CXC chemokine receptor 1 and CXC chemokine receptor 2) as new therapeutic targets[J]. Pharmacol Ther, 2006,112(1):139-149
doi: 10.1016/j.pharmthera.2006.04.002
[15] Wolf M, Delgado MB, Jones SA, Dewald B, Clark-Lewis I, Baggiolini M . Granulocyte chemotactic protein 2 acts via both IL-8 receptors, CXCR1 and CXCR2[J]. Eur J Immunol, 1998,28(1):164-170
doi: 10.1002/(ISSN)1521-4141
[16] Skelton NJ, Quan C, Reilly D, Lowman H . Structure of a CXC chemokine-receptor fragment in complex with interleukin-8[J]. Structure, 1999,7(2):157-168
doi: 10.1016/S0969-2126(99)80022-7 pmid: 10368283
[17] Kido S, Kitadai Y, Hattori N, Haruma K, Kido T, Ohta M, Tanaka S, Yoshihara M, Sumii K, Ohmoto Y, Chayama K . Interleukin 8 and vascular endothelial growth factor — prognostic factors in human gastric carcinomas[J]. Eur J Cancer, 2001,37(12):1482-1487
doi: 10.1016/S0959-8049(01)00147-2
[18] Huang J, Yao JL, Zhang L, Bourne PA , Quinn AM, di Sant’Agnese PA, Reeder JE .Differential expression of interleukin-8 and its receptors in the neuroendocrine and non-neuroendocrine compartments of prostate cancer[J]. Am J Pathol, 2005,166(6):1807-1815
doi: 10.1016/S0002-9440(10)62490-X
[19] Chen PF . Effects of method of heat-clearing and detoxicating on treatment of malignant tumor[J]. Zhejiang Zhong Yi Xue Yuan Xue Bao, 2001,25(5):11-12
陈培丰 . 清热解毒法在恶性肿瘤治疗中的意义和作用机制[J]. 浙江中医学院学报, 2001,25(5):11-12
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