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Journal of Chinese Integrative Medicine ›› 2012, Vol. 10 ›› Issue (8): 874-879.doi: 10.3736/jcim20120807

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Effects of electro-acupuncture at PC6 and BL15 on nerve electrical activity in spinal dorsal root and norepinephrine and dopamine contents in paraventricular nucleus of hypothalamus in rats with acute myocardial ischemia

Meng Li, Ling Hu(), Rong-lin Cai, Zi-jian Wu, Ke-ming Wang#br#   

  1. Institute of Acupuncture, Moxibustion, and Meridians, Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
  • Received:2012-01-13 Accepted:2012-02-02 Online:2012-08-20 Published:2018-08-15
  • Contact: Hu Ling E-mail:hulingtcm@126.com

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Objective: To investigate the effects of electroacupuncture (EA) at Neiguan (PC6) and Xinshu (BL15) on the nerve electrical activity in spinal dorsal root and norepinephrine (NE) and dopamine (DA) concentrations in the paraventricular nucleus of the hypothalamus in rats with acute myocardial ischemia (AMI).

Methods: A total of 100 male Sprague-Dawley rats were randomly divided into sham-operation, model, EA at PC6, EA at BL15 and EA at both PC6 and BL15 groups with 20 rats in each group. The nerve electrical activity in spinal dorsal roots was recorded by bipolar electrodes. NE and DA concentrations in the paraventricular nucleus of the hypothalamus were detected by high-performance liquid chromatography.

Results: : When compared with the sham-operation group, the nerve electrical activity in spinal dorsal roots was significantly increased while the NE and DA concentrations in the paraventricular nucleus of the hypothalamus were decreased in the model group (P<0.01). The nerve electrical activity in spinal dorsal roots was decreased and the NE and DA concentrations were increased in the paraventricular nucleus of the hypothalamus in the EA at PC6 group, the EA at BL15 group and the EA at both PC6 and BL15 group in comparison to those in the model group (P<0.01). The nerve electrical activity in spinal dorsal roots and the NE and DA concentrations in paraventricular nucleus of hypothalamus of the EA at both PC6 and BL15 group were significantly improved when compared to those of the EA at PC6 and EA at BL15 groups (P<0.05).

Conclusion: EA at both PC6 and BL15 acupoints exhibits the synergistic protective effects against AMI. The possible mechanism is related to regulating the nerve electrical activity in spinal dorsal roots and the concentrations of NE and DA in paraventricular nucleus of the hypothalamus.

Key words: point PC6 (Neiguan), point BL15 (Xinshu), ganglia, spinal, norepinephrine, dopamine, myocardial ischemia, electroacupuncture, rats

Table 1

Nerve electrical activity in spinal dorsal roots of rats in different groups ($\bar{x}$±s)"

Group n Frequency (Hz) Amplitude (μV)
Sham-operation 20 480.2±56.6 90.8±10.2
Model 20 640.2±69.4** 220.5±26.4**
Electroacupuncture at BL15 20 571.8±52.1**△△ 159.6±17.9**△△
Electroacupuncture at PC6 20 578.4±60.8**△△ 168.2±18.1**△△
Electroacupuncture at PC6 and BL15 20 504.3±49.5△△▲□ 103.1±11.7△△▲▲□

Table 2

Norepinephrine and dopamine concentrations in paraventricular nucleus of hypothalamus in rats of different groups ($\bar{x}$±s, ng/g)"

Group n Norepinephrine Dopamine
Sham-operation 20 70.81±7.23 161.09±16.62
Model 20 51.01±7.91** 114.22±12.51**
Electroacupuncture at BL15 20 64.17±9.85**△△ 146.72±22.16**△△
Electroacupuncture at PC6 20 63.19±5.22**△△ 131.68±17.15**△△▲
Electroacupuncture at PC6 and BL15 20 69.94±8.17△△▲▲□ 156.51±12.48△△▲▲□
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