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Journal of Chinese Integrative Medicine ›› 2003, Vol. 1 ›› Issue (2): 116-118.doi: 10.3736/jcim20030209

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Hepatocyte apoptosis and the expression of Bcl-2 and Bax in Yin-jaundice rats

Jian-jun Zhang1(), Gan-xiang He2, Chi-zhi Zhang1   

  1. 1. Institute of Liver Diseases, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan,Hubei Province 430061,China
    2. Surgical Department, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan,Hubei Province 430061,China
  • Received:2003-02-17 Online:2003-06-20 Published:2018-10-19


To investigate the effects of Yinchen Shufu Decoction on hepatocyte apoptosis and the expression of its apoptosis-regulating gene Bcl-2 and Bax in Yin-jaundice rats.


Forty-eight rats were divided randomly into 4 groups: the normal control group, Yin-jaundice model group, Yang-jaundice model group, Yinchen Shufu Decoction treatment group. The TUNEL assay and the immunohistochemistry assay were used to detect the apoptosis of hepatocytes and the expression of Bcl-2 and Bax in hepatocytes respectively.


The rate of apoptosis cells in Yin-jaundice model group was higher significantly than that in Yang-jaundice model group and normal control group (P<0.01), the expression of Bcl-2 in Yinchen Shufu Decoction treatment group was higher significantly than that in Yin-jaundice model group (P<0.01 or P<0.05), and the expression of Bax in it was lower significantly than that in Ying-jaundice model group (P<0.01 or P<0.05).


Yinchen Shufu Decoction can prevent hepatocyte apoptosis perhaps by up-regulating the expression of Bcl-2 and down-regulating the expression of Bax. It is one of the mechanisms of its treatment on Yin-jaundice.

Key words: Yin-jaundice, Yinchen Shufu Decoction, hepatocyte, apoptosis, gene expressing

CLC Number: 

  • R256.4


组别 n 凋亡率 Bcl-2阳性细胞率 Bax阳性细胞率
A 7 03.15±01.24 04.59±1.27 15.47±6.57
B 6 38.69±11.44** 18.80±7.24** 37.40±8.75**
C 5 31.75±10.96** 10.89±4.42** 29.60±5.47**
D 6 28.75±12.54 26.95±5.14 25.80±7.22
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