茵陈术附汤对阴黄证大鼠肝细胞凋亡及Bcl-2和Bax表达的影响

doi:10.3736/jcim20030209

Abstract

Objective

To investigate the effects of Yinchen Shufu Decoction on hepatocyte apoptosis and the expression of its apoptosis-regulating gene Bcl-2 and Bax in Yin-jaundice rats.

Mothods

Forty-eight rats were divided randomly into 4 groups: the normal control group, Yin-jaundice model group, Yang-jaundice model group, Yinchen Shufu Decoction treatment group. The TUNEL assay and the immunohistochemistry assay were used to detect the apoptosis of hepatocytes and the expression of Bcl-2 and Bax in hepatocytes respectively.

Results

The rate of apoptosis cells in Yin-jaundice model group was higher significantly than that in Yang-jaundice model group and normal control group (P<0.01), the expression of Bcl-2 in Yinchen Shufu Decoction treatment group was higher significantly than that in Yin-jaundice model group (P<0.01 or P<0.05), and the expression of Bax in it was lower significantly than that in Ying-jaundice model group (P<0.01 or P<0.05).

Conclusion

Yinchen Shufu Decoction can prevent hepatocyte apoptosis perhaps by up-regulating the expression of Bcl-2 and down-regulating the expression of Bax. It is one of the mechanisms of its treatment on Yin-jaundice.

Key words: Yin-jaundice, Yinchen Shufu Decoction, hepatocyte, apoptosis, gene expressing

CLC Number:

R256.4

Jian-jun Zhang, Gan-xiang He, Chi-zhi Zhang. Hepatocyte apoptosis and the expression of Bcl-2 and Bax in Yin-jaundice rats. Journal of Chinese Integrative Medicine, 2003, 1(2): 116-118.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20030209

http://www.jcimjournal.com/EN/Y2003/V1/I2/116

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组别	n	凋亡率	Bcl-2阳性细胞率	Bax阳性细胞率
A	7	03.15±01.24	04.59±1.27	15.47±6.57
B	6	38.69±11.44**^▲	18.80±7.24**^▲	37.40±8.75**
C	5	31.75±10.96**	10.89±4.42**	29.60±5.47**
D	6	28.75±12.54	26.95±5.14^△	25.80±7.22^△

Hepatocyte apoptosis and the expression of Bcl-2 and Bax in Yin-jaundice rats

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