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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (4): 292-294.doi: 10.3736/jcim20040418

• Technique and Methods • Previous Articles     Next Articles

Effects of three different drying methods on extraction and separation of ginsenosides from fresh ginseng

Dai-jia Zhang1, Zhi-long Xiu1, Xin-hua Lin2, Dong-jian Qi2   

  1. 1. Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian, Liaoning Province 116024, China
    2. Sanitation and Epidemic Prevention Station of Shahekou District, Dalian, Liaoning Province 116022, China
  • Received:2003-11-12 Online:2004-07-20 Published:2018-10-20

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Objective: There exists a close relationship between drying of a fresh herb and its preservation and extraction of efficient components. In order to investigate the influences of different drying methods on extraction and separation of ginsenosides, three drying processes, such as drying in the sun, drying in oven and microwave drying, were used to dry fresh ginsengs .Methods: The ginsenosides of the dry ginsengs were extracted by poaching and microwave heating, and were separated by foam separation.The concentrations of ginsenosides were measured.Results: Microwave drying saved both time and labour, and was favorable for release of ginsenosides. The ginsenosides could be extracted from the dry ginsengs in a shorter time by microwave heating than poaching. The ginsenosides Rb1, Rb2, Rd could be observably concentrated by foam separation.Conclusion: Microwave drying and microwave assisted extraction are efficient and economic methods with a high recovery yield of ginsenosides.

Key words: ginseng, ginsenosides, drying, microwave, foam separation

CLC Number: 

  • R283

Fig 1

Pictures of three different kinds of dry ginsengs A: Drying in the sun; B: Drying in oven; C: Microwave drying"

Tab 1

Foam separation results of ginsenosides in three different kinds of poached ginsengs"

Ginseng sample Concentration
of extraction
(mg/ml)		 Volume of
foam phase
(ml)		 Concentration
of foam phase
(mg/ml)		 Concentration of
residue
(mg/ml)		 Multiple of
concentration		 Recovery
yield
(%)
Drying in the sun 0.96 44.0 1.17 0.72 1.22 67.0
Drying in oven 0.75 10.0 1.27 0.71 1.69 21.2
Microwave drying 0.81 7.5 2.16 0.65 2.67 25.5

Tab 2

Foam separation results of ginsenosides in microwave assisted extraction of three different kinds of ginsengs"

Ginseng sample Concentration
of extraction
(mg/ml)		 Volume of
foam phase
(ml)		 Concentration
of foam phase
(mg/ml)		 Concentration
of residue
(mg/ml)		 Multiple of
concentration		 Recovery
yield
(%)
Drying in the sun 0.97 15.0 1.26 0.79 1.30 24.4
Drying in oven 0.83 7.0 1.32 0.69 1.59 13.9
Microwave drying 0.85 5.0 2.25 0.64 2.65 17.0

Tab 3

Test results of mono-ginsenosides in poached microwave dried ginseng"

Item Volume (ml) Concentration
of total
ginsenosides
(mg/ml )		 Concentration of mono-ginsenoside(mg/ml)
Rb1 Rb2 Rc Rd Rf Re Rg1
Extraction 78.5 0.81 0.030 0.002 0.003 0.007 0.009 0.024 0.025
Foam phase 7.5 2.16 0.186 0.056 0.007 0.053 0.017 0.026 0.024
Residue 71.0 0.65 0.004 – – – 0.005 0.025 0.024
Multiple of concentration 2.67 6.20 28.00 2.33 7.57 1.89 1.00 1.00
Recovery yield(%) 25.5 59.0 267.0 22.0 72.0 18.0 9.6 9.6
[1] 上官棣华, 刘国诠 . 人参成分的代谢研究进展[J]. 中草药, 1999,30(11):865-870
doi: 10.7501/j.issn.0253-2670.1999.11.1999011594
[2] 陈发奎 . 常用中草药有效成分含量测定(第1版)[M]. 北京: 人民卫生出版社, 1997,( 第1版):13
[3] 窦德强, 靳玲, 陈英杰 . 人参的化学成分及药理活性的研究进展与展望[J]. 沈阳药科大学学报, 1999,16(4):151-156
doi: 10.3969/j.issn.1006-2858.1999.02.023
[4] 修志龙, 张代佳, 贾凌云 , 等. 泡沫分离法分离人参皂苷[J]. 过程工程学报, 2001,1(3):289-292
doi: 10.3321/j.issn:1009-606X.2001.03.013
[5] 王威, 刘传斌, 修志龙 . 高山红景天皂苷提取新工艺[J]. 中草药, 1999,30(11):824-826
[6] 张代佳, 刘传斌, 修志龙 , 等. 微波技术在植物胞内有效成分提取中的应用[J]. 中草药, 2000,31(9):附5-附6
doi: 10.7501/j.issn.0253-2670.2000.9.446
[7] 魏璐雪, 袁昌鲁 . 中药制剂分析(第1版)[M]. 上海: 上海科学技术出版社, 1997,( 第1版):183
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