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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (6): 445-449.doi: 10.3736/jcim20040612

• Original Experimental Research • Previous Articles     Next Articles

Inhibiting effects of Panax notoginseng extracts on proliferation of GES-1 cells and MNNG-transformed GES-1 cells

Zhi-bin Wang1(), Jun-xiang Li1, Ling-qun Zhu2, Fu-ling Niu2, Wei Cui2   

  1. 1. Department of Gastroenterology, Dongfang Hospital of Beijing University of Traditional Chinese Medicine, Beijing 100078, China
    2. Laboratory of Internal Medicine, Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100700, China
  • Received:2004-08-30 Online:2004-11-20 Published:2018-10-20

Objective: Through cell cultivation, we studied the inhibiting effects of the serum of the dog fed with Panax notoginseng extracts on precancerous gastric cells, trying to find the best time points or periods when the extracts' function was the strongest after administration of the extracts to the dog.Methods: The experiments adopted eternalized human gastric mucosa epithelium GES-1 cells and MC cells gained from GES-1 cells transformed by N-methyl-N'-nitro-N-nitroso-guanidine (MNNG) as the model of precancerous lesions for study in vitro. We took the serum of a dog before and at different points of time after feeding the dog with Panax notoginseng extracts for experiment. By means of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay, we examined the inhibiting effects of the serum after culturing the GES-1 and MC cells for 72 hours with different concentration (8%, 4%, 2%) of medicated serum obtained from the dog at different points of time, so as to find that, at which points of time the medicated serum obtained, it would be the most effective.Results: The results showed that the GES-1 and MC cells inhibition rates of medicated serum from the points of 2-hour and 6-hour were the highest, and the culture medium containing 8% of medicated serum from these two points had prominent inhibiting effects on both kinds of cells. The GES-1 cells inhibition rate in culture medium containing 8% of medicated serum from the point of 2-hour was 70.8% (P<0.01) and that of the MC cells was 45.3% (P<0.01). The GES-1 cells inhibition rate in culture medium containing 8% of medicated serum from the point of 6-hour was 88.5% (P<0.01) and that of the MC cells was 42.4% (P<0.01).Conclusion: The points of time with the strongest inhibiting effects are 2 hours and 6 hours after being fed with Panax notoginseng extracts. At these two points, the serum is most effective in inhibiting the proliferation of GES-1 and MC cells.

Key words: Panax notoginseng, gastric cancer, cell culture, animals, laboratory

CLC Number: 

  • R285.6

Fig 1

GES-1 cells cultivated for 72 hours in culture medium containing normal serum (×200)"

Fig 2

GES-1 cells cultivated for 72 hours in culture medium containing 4% of medicated serum from the point of 2-hour (×100)"

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Fig 3

MC cells cultivated for 72 hours in culture medium containing normal serum (×100)"

Fig 4

MC cells cultivated for 72 hours in culture medium containing 4% of medicated serum from the point of 2-hour (×100)"

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[1] 陈洁平, 林晨, 张雪艳 , 等. 维甲酸诱导基因RA538诱导人胃癌细胞系凋亡及其分子机制的研究[J]. 中华微生物学和免疫学杂志, 2000,6(20):551-555
doi: 10.3760/j:issn:0254-5101.2000.06.024
[2] 沈洪薰, 陈莉, 周树军 , 等. 复方参七汤治疗胃癌的临床与实验研究[J]. 世界华人消化杂志, 1998,6(10):837-840
doi: 10.3969/j.issn.1009-3079.1998.10.004
[3] 石雪迎, 赵凤志, 戴欣 , 等. 三七对胃癌前病变大鼠胃黏膜癌基因蛋白异常表达的影响[J]. 北京中医药大学学报, 2001,24(6):37-39
doi: 10.3321/j.issn:1006-2157.2001.06.013
[4] 苏秀兰, 柯杨, 舍英 , 等. 亚硝胺类化合物MNNG诱导人胃黏膜上皮细胞系GES-1恶性转化的研究[J]. 内蒙古医学杂志, 1995,15(2):65-67
[5] 柯杨, 宁涛, 王冰 , 等. 人胃黏膜上皮细胞系GES-1的建立及其生物学特性[J]. 中华肿瘤杂志, 1994,16(1):7-8
[6] 苏秀兰, 宁涛, 柯杨 . 化学致癌剂MNNG对人胃黏膜上皮细胞骨架作用的研究[J]. 解剖学报, 1995,26(4):291-293
doi: 10.1007/BF02007173
[7] 石雪迎, 赵凤志, 戴欣 , 等. 三七总皂甙对转化的人胃黏膜上皮细胞增殖的抑制作用[J]. 北京中医药大学学报, 2001,24(5):29-33
doi: 10.3321/j.issn:1006-2157.2001.05.010
[8] 章静波 . 细胞生物学实用方法与技术(第1版)[M]. 北京: 北京医科大学协和医科大学联合出版社, 1995,( 第1版):85-86
[9] 马郁琪, 肖建初 . 3H三七皂甙RG1在大鼠体内的药代动力学研究 [J]. 中草药, 1987,18(9):21-24
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