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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (11): 1078-1085.doi: 10.3736/jcim20091111

• Original Experimental Research • Previous Articles     Next Articles

Effects of plasma containing Buyang Huanwu Decoction and its effective components and total Panax notoginseng saponins on vascular smooth muscle cell proliferation induced by platelet-derived growth factor

 Hua Lia,Gang Chenb,Chang-qing Dengb   

  1. a Department of Histology and Embryology, Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China
    b Laboratory of Pathophysiology, Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China
  • Received:2009-07-31 Accepted:2009-09-15 Online:2009-11-20 Published:2009-11-15
  • Contact: Chang-qing Deng E-mail:dchangq@yahoo.cn

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Objective

To explore the effects of plasma containing Buyang Huanwu Decoction (BYHWD), a compound of traditional Chinese medicine, and its effective components (alkaloids and glycosides) and total Panax notoginseng saponins (TSPN) on vascular smooth muscle cell (VSMC) proliferation induced by platelet-derived growth factor (PDGF) in vitro.
Methods

VSMC proliferation was induced by incubating VSMCs with PDGF-BB. Effects of different concentrations of blank plasma on the VSMC proliferation and 50% inhibitory concentrations of plasma containing BYHWD, its alkaloids and glycosides, TSPN and atorvastatin were detected by using methyl thiazolyl tetrazolium (MTT) method to determine the concentrations of plasma containing drugs for the following experiments. Then, MTT and flow cytometry methods were used to detect the effects of plasma containing BYHWD, its alkaloids and glycosides, TSPN and atorvastatin on VSMC proliferative activity and cell cycles.
Results

VSMC proliferative activity was enhanced obviously after PDGF-BB stimulation. The cell number in G0/G1 phase was decreased and the cell quantity in G2/M and S phases was increased following PDGF-BB stimulation. Compared with PDGF-BB stimulation group, VSMC proliferation induced by PDGF-BB in plasma containing BYHWD, its alkaloids and glycosides, TSPN and atorvastatin groups was inhibited. In all plasma containing drug components groups, the cell number of G0/G1 phase was higher and the cell quantity of G2/M and S phases was lower than those in the PDGF-BB stimulation group.
Conclusion

BYHWD and its alkaloids and glycosides have similar roles of resisting VSMC proliferation to TSPN and atorvastatin. Alkaloids and glycosides may be the major effective substances of anti-VSMC proliferation in BYHWD.

Key words: Buyang Huanwu Decoction, Alkaloids, Glycoside, Total Panax notoginseng saponins, Plasma pharmacology, Vascular smooth muscle cell, Cell proliferation

Figure 1

VSMC growth-inhibitory curve of blank plasma"

Table 1

IC50 of plasma containing various components on VSMC proliferation induced by PDGF-BB ($\bar{x}$±s, %)"

Group n IC50
Plasma containing atorvastatin 5 10.0±0.9
Plasma containing alkaloids 5 11.0±1.3
Plasma containing glycosides 5 10.0±1.2
Plasma containing BYHWD 5 10.0±0.9
Plasma containing TSPN 5 11.0±0.8

Figure 2

VSMC growth-inhibitory curves of plasma containing various components induced by PDGF-BB A: Plasma containing alkaloids; B: Plasma containing glycosides; C: Plasma containing BYHWD; D: Plasma containing TSPN; E: Plasma containing atorvastatin."

Table 2

Effects of plasma containing various components on VSMC proliferation induced by PDGF-BB ($\bar{x}$±s)"

Group n OD (490 nm)
0 h 12 h 24 h 48 h
Blank control 3 0.381±0.005 0.401±0.019 0.518±0.011 0.619±0.016
PDGF-BB stimulation 3 0.566±0.020* 0.796±0.013* 0.969±0.016*
Blank plasma control 3 0.526±0.005*△ 0.759±0.003*△ 0.892±0.040*△
Plasma containing atorvastatin 3 0.469±0.020*▲ 0.660±0.230*▲ 0.759±0.025*▲
Plasma containing alkaloids 3 0.467±0.017*▲ 0.667±0.011*▲ 0.765±0.007*▲
Plasma containing glycosides 3 0.490±0.010*▲ 0.678±0.003*▲ 0.781±0.007*▲
Plasma containing BYHWD 3 0.465±0.014*▲ 0.678±0.003*▲ 0.760±0.023*▲
Plasma containing TSPN 3 0.483±0.015*▲ 0.679±0.003*▲ 0.785±0.005*▲

Table 3

Effects of plasma containing various components on VSMC proliferative cycle ($\bar{x}$±s %)"

Group n Cell cycle
G0/G1 G2/M+S
Blank control 3 73.08±1.74 26.92±1.74
PDGF-BB stimulation 3 49.60±1.38** 50.41±1.38*
Blank plasma control 3 55.31±0.26*△ 44.69±0.26*△
Plasma containing atorvastatin 3 80.64±4.94*▲▲ 19.36±4.94*▲▲
Plasma containing alkaloids 3 83.91±4.02*▲▲ 16.09±4.02*▲▲
Plasma containing glycosides 3 81.66±1.17*▲▲ 18.68±1.28*▲▲
Plasma containing BYHWD 3 83.50±5.66*▲▲ 16.50±5.66*▲▲
Plasma containing TSPN 3 79.63±2.15*▲▲ 20.37±2.15*▲▲
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