Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (11): 1078-1085.doi: 10.3736/jcim20091111

• Original Experimental Research • Previous Articles     Next Articles

Effects of plasma containing Buyang Huanwu Decoction and its effective components and total Panax notoginseng saponins on vascular smooth muscle cell proliferation induced by platelet-derived growth factor

 Hua Lia,Gang Chenb,Chang-qing Dengb   

  1. a Department of Histology and Embryology, Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China
    b Laboratory of Pathophysiology, Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China
  • Received:2009-07-31 Accepted:2009-09-15 Online:2009-11-20 Published:2009-11-15
  • Contact: Chang-qing Deng E-mail:dchangq@yahoo.cn

Objective

To explore the effects of plasma containing Buyang Huanwu Decoction (BYHWD), a compound of traditional Chinese medicine, and its effective components (alkaloids and glycosides) and total Panax notoginseng saponins (TSPN) on vascular smooth muscle cell (VSMC) proliferation induced by platelet-derived growth factor (PDGF) in vitro.
Methods

VSMC proliferation was induced by incubating VSMCs with PDGF-BB. Effects of different concentrations of blank plasma on the VSMC proliferation and 50% inhibitory concentrations of plasma containing BYHWD, its alkaloids and glycosides, TSPN and atorvastatin were detected by using methyl thiazolyl tetrazolium (MTT) method to determine the concentrations of plasma containing drugs for the following experiments. Then, MTT and flow cytometry methods were used to detect the effects of plasma containing BYHWD, its alkaloids and glycosides, TSPN and atorvastatin on VSMC proliferative activity and cell cycles.
Results

VSMC proliferative activity was enhanced obviously after PDGF-BB stimulation. The cell number in G0/G1 phase was decreased and the cell quantity in G2/M and S phases was increased following PDGF-BB stimulation. Compared with PDGF-BB stimulation group, VSMC proliferation induced by PDGF-BB in plasma containing BYHWD, its alkaloids and glycosides, TSPN and atorvastatin groups was inhibited. In all plasma containing drug components groups, the cell number of G0/G1 phase was higher and the cell quantity of G2/M and S phases was lower than those in the PDGF-BB stimulation group.
Conclusion

BYHWD and its alkaloids and glycosides have similar roles of resisting VSMC proliferation to TSPN and atorvastatin. Alkaloids and glycosides may be the major effective substances of anti-VSMC proliferation in BYHWD.

Key words: Buyang Huanwu Decoction, Alkaloids, Glycoside, Total Panax notoginseng saponins, Plasma pharmacology, Vascular smooth muscle cell, Cell proliferation

Figure 1

VSMC growth-inhibitory curve of blank plasma"

Table 1

IC50 of plasma containing various components on VSMC proliferation induced by PDGF-BB ($\bar{x}$±s, %)"

Group n IC50
Plasma containing atorvastatin 5 10.0±0.9
Plasma containing alkaloids 5 11.0±1.3
Plasma containing glycosides 5 10.0±1.2
Plasma containing BYHWD 5 10.0±0.9
Plasma containing TSPN 5 11.0±0.8

Figure 2

VSMC growth-inhibitory curves of plasma containing various components induced by PDGF-BB A: Plasma containing alkaloids; B: Plasma containing glycosides; C: Plasma containing BYHWD; D: Plasma containing TSPN; E: Plasma containing atorvastatin."

Table 2

Effects of plasma containing various components on VSMC proliferation induced by PDGF-BB ($\bar{x}$±s)"

Group n OD (490 nm)
0 h 12 h 24 h 48 h
Blank control 3 0.381±0.005 0.401±0.019 0.518±0.011 0.619±0.016
PDGF-BB stimulation 3 0.566±0.020* 0.796±0.013* 0.969±0.016*
Blank plasma control 3 0.526±0.005*△ 0.759±0.003*△ 0.892±0.040*
Plasma containing atorvastatin 3 0.469±0.020*▲ 0.660±0.230*▲ 0.759±0.025*
Plasma containing alkaloids 3 0.467±0.017*▲ 0.667±0.011*▲ 0.765±0.007*
Plasma containing glycosides 3 0.490±0.010*▲ 0.678±0.003*▲ 0.781±0.007*
Plasma containing BYHWD 3 0.465±0.014*▲ 0.678±0.003*▲ 0.760±0.023*
Plasma containing TSPN 3 0.483±0.015*▲ 0.679±0.003*▲ 0.785±0.005*

Table 3

Effects of plasma containing various components on VSMC proliferative cycle ($\bar{x}$±s %)"

Group n Cell cycle
G0/G1 G2/M+S
Blank control 3 73.08±1.74 26.92±1.74
PDGF-BB stimulation 3 49.60±1.38** 50.41±1.38*
Blank plasma control 3 55.31±0.26*△ 44.69±0.26*△
Plasma containing atorvastatin 3 80.64±4.94*▲▲ 19.36±4.94*▲▲
Plasma containing alkaloids 3 83.91±4.02*▲▲ 16.09±4.02*▲▲
Plasma containing glycosides 3 81.66±1.17*▲▲ 18.68±1.28*▲▲
Plasma containing BYHWD 3 83.50±5.66*▲▲ 16.50±5.66*▲▲
Plasma containing TSPN 3 79.63±2.15*▲▲ 20.37±2.15*▲▲
[1] Xue J, Xie ML . Advances in methodology in serum pharmacology of Chinese materia medica[J]. Zhong Cao Yao, 2003,34(6):U009-U011
doi: 10.3321/j.issn:0253-2670.2003.06.043
薛洁, 谢梅林 中药血清药理学的方法学研究近况[J].中草药, 2003, 34(6):附009-附011
doi: 10.3321/j.issn:0253-2670.2003.06.043
[2] He SL, Ge JW, He R, Mei ZG . Doubt the validity of serum pharmacology, emphasize the ex vivo experiments on multiple levels[J]. Zhongguo Yao Li Xue Tong Bao, 2005,21(3):277-279
贺石林, 葛金文, 贺蓉, 梅志刚 . 质疑血清药理学, 加强多层次半体内实验研究[J]. 中国药理学通报, 2005,21(3):277-279
[3] Wu L, Zhang W, Li H, Zhang GM, Chen BY, Tang YH, Deng CQ . Effects of Buyang Huanwu Decoction and its alkaloids and glycosides on aortic intimal hyperplasia and expression of proliferating cell nuclear antigen in rats with aortic intimal injuries[J]. J Chin Integr Med, 2008,6(8):836-842
吴露, 张伟, 李花, 张国民, 陈北阳, 唐映红, 邓常清 . 补阳还五汤及其有效组分生物碱和苷对大鼠血管内膜损伤后主动脉内膜增生和增殖细胞核抗原表达的影响[J]. 中西医结合学报, 2008,6(8):836-842
[4] Wu L, Zhang W, Li H, Chen BY, Tang YH, He FY, Deng CQ . Inhibition of aortic intimal hyperplasia and cell cycle protein and extracellular matrix protein expressions by Buyang Huanwu Decoction[J]. J Ethnopharmacol, 2009,125(3):423-435
doi: 10.1016/j.jep.2009.07.022
[5] Lin SG, Zheng XL, Chen QY, Sun JJ . Effect of Panax notoginseng saponins on increased proliferation of cultured aortic smooth muscle cells stimulated by hypercholesterolemic serum[J]. Zhongguo Yao Li Xue Bao, 1993,14(4):314-316
[6] Wu L, Zhang W, Tang YH, Li H, Chen BY, Zhang GM, Deng CQ .Effect of total saponins of "Panax notoginseng root" on aortic intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix in rats[J].Phytomedicine , 2009,(Sep 10):[Epub ahead of print]
[7] Ma QL, Sun M, Yang TL, Li YJ, Tang CE, Peng ZY, He SL, Chen FP . Effects of Tongxinluo on cell viability and tissue factor in Ang Ⅱ induced vascular endothelial cells[J]. Zhong Nan Da Xue Xue Bao Yi Xue Ban, 2007,32(3):485-488
马琦琳, 孙明, 杨天山仑, 李元建, 汤参娥, 彭振宇, 贺石林, 陈方平 . 通心络对血管紧张素Ⅱ诱导的血管内皮细胞活力及组织因子的影响[J]. 中南大学学报(医学版), 2007,32(3):485-488
[8] Iwama H, Amagaya S, Ogihara Y . Effect of shosai-koto, a Japanese and Chinese traditional herbal medicinal mixture, on the mitogenic activity of lipopolysaccharide: a new pharmacological testing method[J]. J Ethnopharmacol, 1987,21(1):45-53
doi: 10.1016/0378-8741(87)90093-6
[9] Kim MJ, Park KG, Lee KM, Kim HS, Kim SY, Kim CS, Lee SL, Chang YC, Park JY, Lee KU, Lee IK . Cilostazol inhibits vascular smooth muscle cell growth by downregulation of the transcription factor E2F[J]. Hypertension, 2005,45(4):552-556
doi: 10.1161/01.HYP.0000158263.64320.eb
[10] Peters S, Gotting B, Trümmel M, Rust H, Brattstrom A . Valsartan for prevention of restenosis after stenting of type B2/C lesions: the VAL-PREST trial[J]. J Invasive Cardiol, 2001,13(2):93-97
[11] Walter DH, Schachinger V, Elsner M, Mach S, Auch-Schwelk W, Zeiher AM . Effect of statin therapy on restenosis after coronary stent implantation[J]. Am J Cardiol, 2000,85(8):962-968
doi: 10.1016/S0002-9149(99)00910-8 pmid: 11054944
[12] Pan SL, Huang YW, Guh JH, Chang YL, Peng CY, Teng CM . Esculetin inhibits Ras-mediated cell proliferation and attenuates vascular restenosis following angioplasty in rats[J]. Biochem Pharmacol, 2003,65(11):1897-1905
doi: 10.1016/S0006-2952(03)00161-8
[13] Kim DW, Park YS, Kim YG, Piao H, Kwon JS, Hwang KK, Youn TJ, Park JB, Yun YP, Sachinidis A, Kim CH, Cho MC, Ahn HY . Local delivery of green tea catechins inhibits neointimal formation in the rat carotid artery injury model[J]. Heart Vessels, 2004,19(5):242-247
[1] Syed Nasir Abbas Bukhari, Fahad Hussain, Hnin Ei Thu, Zahid Hussain. Synergistic effects of combined therapy of curcumin and Fructus Ligustri Lucidi for treatment of osteoporosis: cellular and molecular evidence of enhanced bone formation. Journal of Integrative Medicine, 2019, 17(1): 38-45.
[2] Madan Mohan Pandey, Sayyada Khatoon, Subha Rastogi, Ajay Kumar Singh Rawat. Determination of flavonoids, polyphenols and antioxidant activity of Tephrosia purpurea: A seasonal study. Journal of Integrative Medicine, 2016, 14(6): 447-455.
[3] Dao-fang Ding , Song-pu Wei, Xiao-feng Li , Xiao-gang Zhang , Hong-sheng Zhan , Tie-li Duan , Yue-long Cao. Inhibition effect of osthole on proliferation of rat chondrocytes. Journal of Chinese Integrative Medicine, 2012, 10(12): 1413-1418.
[4] Mukherjee Avinaba, Sikdar Sourav, Bishayee Kausik, Paul Avijit, Ghosh Samrat, Boujedaini Naoual, Rahman Khuda-Bukhsh Anisur. Ethanolic extract of Thuja occidentalis blocks proliferation of A549 cells and induces apoptosis in vitro. Journal of Chinese Integrative Medicine, 2012, 10(12): 1451-1459.
[5] Li Zhang , Tao Wu , Jun-ming Chen , Li-li Yang , Hai-yan Song , Guang Ji. Danshensu inhibits acetaldehyde-induced proliferation and activation of hepatic stellate cell-T6. Journal of Chinese Integrative Medicine, 2012, 10(10): 1155-.
[6] Han Xiang-hui, Ye Yi-yi, Guo Bao-feng, Liu Sheng. Effects of platycodin D in combination with different active ingredients of Chinese herbs on proliferation and invasion of 4T1 and MDA-MB-231 breast cancer cell lines. Journal of Chinese Integrative Medicine, 2012, 10(1): 67-75.
[7] Zhao-hui Chu, Xiao-hua Liang, Xin-li Zhou, Ruo-fan Huang, Qiong Zhan, Jing-wei Jiang. Effects of deguelin on proliferation and apoptosis of MCF-7 breast cancer cells by phosphatidylinositol 3-kinase/Akt signaling pathway. Journal of Chinese Integrative Medicine, 2011, 9(5): 533-538.
[8] Bao Tan, Hai-lian Shi, Guang Ji, Lan Lu, Ai-li Cao, Song-shan Shi, Jian-qun Xie. Antiproliferative effects of essential oil of a compound Chinese herbal medicine Weiqi Decoction on AGS cells. Journal of Chinese Integrative Medicine, 2011, 9(5): 558-564.
[9] Bo Yang, Xue-chun Lu, Feng Zhang, Yang Liu, Hong-li Zhu. Inhibitory effects of traditional Chinese herbal medicine Tanreqing injection on proliferation of leukemia cells in vitro and the potential mechanisms. Journal of Chinese Integrative Medicine, 2011, 9(4): 414-422.
[10] Mei-na Ye, Hong-feng Chen, Rui-juan Zhou, Ming-juan Liao. Effects of Astragalus polysaccharide on proliferation and Akt phosphorylation of the basal-like breast cancer cell line. Journal of Chinese Integrative Medicine, 2011, 9(12): 1339-1346.
[11] Wei-yi Gong , Xin-min Zhang, Zi-yin Shen, Guo-rang Hu, Su-qin Zhang, Run-hong Liu, Wei-dong Zhang, Jing-cheng Dong. Optimal combination of baicalin, icariin and Astragalus saponin Ⅰ from a Chinese herbal compound Biminne. Journal of Chinese Integrative Medicine, 2010, 8(6): 541-547.
[12] Bing Hu, Hong-mei An, Ke-ping Shen, Qin Du. Effects of Tenglong Buzhong Decoction on proliferation and apoptosis of human colon carcinoma cell line LS174T. Journal of Chinese Integrative Medicine, 2010, 8(6): 575-580.
[13] Hai-bin Deng, Zhong-qi Wang , Zhen-ye Xu. Inhibitory effects of Feiyanning Decoction on proliferation, migration and tube formation of human umbilical vein endothelial cells in vitro. Journal of Chinese Integrative Medicine, 2009, 7(3): 255-260.
[14] Ying Deng , Hong-feng Chen. Effects of Astragalus injection and its ingredients on proliferation and Akt phosphorylation of breast cancer cell lines. Journal of Chinese Integrative Medicine, 2009, 7(12): 1174-1180.
[15] Ming-yu Zhang, Zhi-bin Shi, Hua-qing Yang, Shu-qiang Ma, Wei Wang, Xiao-qian Dang, Kun-zheng Wang. Effects of different doses of puerarin on vascular endothelial cell proliferation in vitro. Journal of Chinese Integrative Medicine, 2008, 6(9): 942-945.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[2] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[3] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[4] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[5] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Chan Chen, Yong-mei Meng , Peng Zhang, Juan Wang, Hui-hui Zhao, Shu-zhen Guo , Wei Wang. Diagnosis and treatment rule of traditional Chinese medicine for syndrome factors of chronic congestive heart failure: a study based on Shannon entropy method. Journal of Chinese Integrative Medicine, 2010, 8(11): 1080-1084
[10] Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210