SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (5): 367-371.doi: 10.3736/jcim20040517

• Original Experimental Research • Previous Articles     Next Articles

Effect of Bugu Mixture on all-trans retinoic acid-induced apoptosis of bone marrow stromal cells

Nan Li1(), He-ming Wang1, Xu Lin2, Liang-pu Zheng3, Lin Shen4, Yi-ping Lin5, Lin-ying Zhou6, Xiu-rong Zhong6, Fang Lin7   

  1. 1. Department of Orthopedics and Traumatology, Fujian Traditional Chinese Medical College, Fuzhou, Fujian Province 350003, China
    2. Department of Orthopedics and Traumatology, Fujian Traditional Chinese Medical College, Fuzhou, Fujian Province 350003, China
    3. Molecular Medicine Center, Fujian Medical University, Fuzhou, Fujian Province 350004, China
    4. Medical Science Experiment Center, Fujian Traditional Chinese Medical College, Fuzhou, Fujian Province 350003, China
    5. Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430022, China
    6. Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province 430022, China
    7. Department of Traditional Chinese Basic Medicine, Fujian Academy of Traditional Chinese Medicine, Fuzhou, Fujian Province 350003, China
  • Received:2003-11-05 Online:2004-09-20 Published:2018-10-20

RichHTML

1

PDF (PC)

80

Like

Objective: We used the SD rat's bone marrow stromal cells (BMSCs) cultured in vitro to observe the effects of Bugu Mixture on the apoptosis and to explore the molecular biologic mechanism of the treatment of osteoporosis with Bugu Mixture.Methods: BMSCs were separated from the bones of the extremities of SD rats in vitro. The morphologic changes, the apoptosis cell cycles, the mitochondrion membrane potential changes, and the Bcl-2 and Bax gene expression were observed, and the effects of Bugu Mixture on the course of cell apoptosis were evaluated.Results: The earlier use of Bugu Mixture could decrease the cells blocked in G0/G1 phase, and promote their synthesis of DNA in S phase. The expression of Bcl-2 was higher in the Bugu Mixture group than that in the all-trans retinoic acid(ATRA) induced group, and the expression of Bax was lower in the Bugu Mixture group than that in the ATRA induced group. The mitochondrion membrane potential descended significantly in the Bugu Mixture group than that in the ATRA induced group.Conclusion: The mechanism of the treatment of osteoporosis with Bugu Mixture is that the earlier use of Bugu Mixture can decrease the amount of apoptostic cells induced by ATRA, thus promoting the cell mitosis and restraining the apoptosis. It can also act as a protector to Bcl-2 located on the mitochondrion membrane. By preventing the transferring of the Bax protein from cell-plasm to mitochondrion membrane, it takes the advantage of Bcl-2 in forming Bcl-2/Bax homodimer so as to prevent the opening of the permeability transition pore to avoid the changing of mitochondrion membrane potential and the destruction of biosynthesis caused by the mitochondrion release of apoptosis inducing factors and to reach the objective of restraining apoptosis.

Key words: osteoporosis, bone marrow cells, apoptosis, genes, Bcl-2, mitochondria, membrane potentials, animals, laboratory

CLC Number: 

  • R289.5

Fig 1

Morphosis of BMSC apoptosis induced by 10-4 mol/L ATRA (Dyeing by AO-EB, fluorescence microscope, ×1 000)"

Fig 2

Bcl-2 dyeing in control group （×100）"

Fig 3

Bax dyeing in ATRA group （×100）"

Fig 4

Result of FCM detection A: Control group; B: ATRA induced group; C: ATRA and Bugu Mixture group; D: Bugu Mixture (3 d) and ATRA group"

Tab 1

Effect of different treatment on BMSC cycle and apoptosis rate ($\bar{x}$±s, %)"

Group n G1 G2 S Apoptosis rate
Control group 6 57.27±2.38 11.17±3.32 31.61±2.15 0
ATRA induced group 6 82.76±4.90** 10.98±4.49 6.26±1.26** 37.07±2.81
ATRA and Bugu Mixture group 6 68.15±3.87**△△ 21.29±7.51 11.56±3.47**△△ 15.21±3.87△△
Bugu Mixture (3d) and ATRA group 6 60.60±7.15▲ 20.18±3.81 19.23±3.70**▲▲ 7.36±1.68△△▲▲

Tab 2

Effect of different treatment on BMSC ΔΨm ($\bar{x}$±s, %)"

Group n FITC positive rate of BMSC
24 h 48 h
Control group 5 0.58±0.16 0.65±0.38
ATRA induced group 5 6.91±0.45** 62.79±1.48**
Bugu Mixture group 5 4.06±0.46**△△ 24.40±1.64**△△

Tab 3

Relative expression quantity of Bax and Bcl-2 mRNA at different time in different groups"

Group Time Bcl-2 Bax Bcl-2/Bax
Control group
0.227 4 0.100 0 2.274 0
ATRA induced group
1st day 0.082 7 0.332 3 0.248 9
2nd day 0.059 3 0.621 6 0.095 4
3rd day 0.039 1 0.632 2 0.061 9
ATRA and Bugu Mixture group
1st day 0.136 7 0.118 0 1.158 5
2nd day 0.107 5 0.279 6 0.384 5
3rd day 0.150 0 0.205 9 0.728 5
Bugu Mixture (3d) and ATRA group
1st day 0.219 6 0.074 5 2.947 7
2nd day 0.150 4 0.540 9 0.278 1
3rd day 0.124 1 0.257 1 0.482 7
[1] 席越, 王戈平, 黄啸原 . 骨组织病理解剖学技术(第1版)[M]. 北京: 人民卫生出版社, 1997,( 第1版):63-64
[2] 彭黎明, 王曾礼 . 细胞凋亡的基础与临床(第1版)[M]. 北京: 人民卫生出版社, 2000,( 第1版):171-172
[3] Li PF, Dietz R, von Harsdorf R . p53 regulates mitochondrial membrane potential through reactive oxygen species and induces cytochrome c-independent apoptosis blocked by Bcl-2[J]. EMBO J, 1999,18(21):6027-6036
doi: 10.1093/emboj/18.21.6027
[4] Cheng EH, Kirsch DG, Clem RJ , et al. Conversion of Bcl-2 to a Bax-like death effector by caspases[J]. Science, 1997,278(5345):1966-1968
doi: 10.1126/science.278.5345.1966
[5] Reed JC . Double identity for proteins of the Bcl-2 family[J]. Nature, 1997,387(6635):773-776
doi: 10.1038/42867
[1] Cai-lian Fana, Wan-jun Caib, Meng-nan Ye, Miao Chen, Yi Dai. Qili Qiangxin, a compound herbal medicine formula, alleviates hypoxia-reoxygenation-induced apoptotic and autophagic cell death via suppression of ROS/AMPK/mTOR pathway in vitro. Journal of Integrative Medicine, 2022, 20(4): 365-375.
[2] Hong-xiao Li, Ling Shi, Shang-jie Liang Chen-chen Fang, Qian-qian Xu, Ge Lu, Qian Wang, Jie Cheng, Jie Shen, Mei-hong Shen. Moxibustion alleviates decreased ovarian reserve in rats by restoring the PI3K/AKT signaling pathway. Journal of Integrative Medicine, 2022, 20(2): 163-172.
[3] Mohamed S. Othman, Sofian T. Obeidat, Amal H. Al-Bagawi, Mohamed A. Fareid, Alaa Fehaid, Ahmed E. Abdel Moneim. Green-synthetized selenium nanoparticles using berberine as a promising anticancer agent#br#
#br#
. Journal of Integrative Medicine, 2022, 20(1): 65-72.
[4] Joon-ho Lee, Yuan-ji Wei, Zhong-yan Zhou, Yu-ming Hou, Cheng-long Wang, Li-bo Wang, Hong-jin Wu, Yu Zhang, Wei-wei Dai. Efficacy of the herbal pair, Radix Achyranthis Bidentatae and Eucommiae Cortex, in preventing glucocorticoid-induced osteoporosis in the zebrafish model. Journal of Integrative Medicine, 2022, 20(1): 83-90.
[5] Hyongjun Jeon, Hee-Young Kim, Chang-Hwan Bae, Yukyung Lee, Sungtae Koo, Seungtae Kim. Korean red ginseng decreases 1-methyl-4-phenylpyridinium-induced mitophagy in SH-SY5Y cells. Journal of Integrative Medicine, 2021, 19(6): 537-544.
[6] Victor Abou Nehmi Filho, Gilson Masahiro Murata, Ruan Carlos Macêdo de Moraes, Gabriely Cristina Alves Lima, Danielle Araujo de Miranda, Katrin Radloff, Raquel Galvão Figuerêdo Costa, Joyce de Cassia Rosa de Jesus, Jéssica Alves De Freitas, Nayara Izabel Viana, Ruan Pimenta, Katia Ramos Moreira Leite, José Pinhata Otoch, Ana Flávia Marçal Pessoa. A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice. Journal of Integrative Medicine, 2021, 19(5): 439-450.
[7] Ju-Yeon Park, Young-Won Kwon, Sun-Ah Kim, Sun-Dong Park, Chang-Hyun Kim, Jin-Hee Kim, Ju-Hee Lee. Polyherbal formula SC-E3 inhibits rheumatoid arthritis activity in a mouse model of type-II collagen-induced arthritis. Journal of Integrative Medicine, 2021, 19(3): 265-273.
[8] Mu-yan Kong, Le-yan Li, Yan-mei Lou, Hong-yu Chi, Jin-jun Wu. Chinese herbal medicines for prevention and treatment of colorectal cancer: from molecular mechanisms to potential clinical applications. Journal of Integrative Medicine, 2020, 18(5): 369-384.
[9] Porwornwisit Tritripmongkol, Tullayakorn Plengsuriyakarn, Mayuri Tarasuk, Kesara Na-Bangchang. In vitro cytotoxic and toxicological activities of ethanolic extract of Kaempferia galanga Linn. and its active component, ethyl-p-methoxycinnamate, against cholangiocarcinoma. Journal of Integrative Medicine, 2020, 18(4): 326-333.
[10] Varuni Colamba Pathiranage, Ira Thabrew, Sameera R. Samarakoon, Kamani H. Tennekoon, Umapriyatharshini Rajagopalan, Meran K. Ediriweera. Evaluation of anticancer effects of a pharmaceutically viable extract of a traditional polyherbal mixture against non-small-cell lung cancer cells. Journal of Integrative Medicine, 2020, 18(3): 242-252.
[11] Olubukola Titilope Oyebode, Foluso Olayemi Ogunbiyi, Olufunso Olabode Olorunsogo. Opening of liver mitochondrial permeability transition pore in streptozotocin-induced diabetic rats and its inhibition by methanol fraction of Ficus mucoso (Welw) root bark. Journal of Integrative Medicine, 2019, 17(6): 446-454.
[12] Maged Mohamed Maher Abou-Hashem, Dina Mohamed Abo-elmatty, Noha Mostafa Mesbah, Ahmed Mohamed Abd EL-Mawgoud. Induction of sub-G0 arrest and apoptosis by seed extract of Moringa peregrina (Forssk.) Fiori in cervical and prostate cancer cell lines. Journal of Integrative Medicine, 2019, 17(6): 410-422.
[13] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Apirada Sucontphunt, Mattaka Khongkow, Thongchai Koobkokkruad, Nontima Vardhanabhuti. Flavonoids kaempferide and 4,2′-dihydroxy-4′,5′,6′-trimethoxychalcone inhibit mitotic clonal expansion and induce apoptosis during the early phase of adipogenesis in 3T3-L1 cells. Journal of Integrative Medicine, 2019, 17(4): 288-295.
[14] Shriniwas S. Basaiyye, Sanjay Kashyap, Kannan Krishnamurthi, Saravanadevi Sivanesan. Induction of apoptosis in leukemic cells by the alkaloid extract of garden cress (Lepidium sativum L.). Journal of Integrative Medicine, 2019, 17(3): 221-228.
[15] Carlos R. Oliveira, Daniel G. Spindola, Daniel M. Garcia, Adolfo Erustes, Alexandre Bechara, Caroline Palmeira-dos-Santos, Soraya S. Smaili, Gustavo J.S. Pereira, André Hinsberger, Ezequiel P. Viriato, Maria Cristina Marcucci, Alexandra C.H.F. Sawaya, Samantha L. Tomaz, Elaine G. Rodrigues, Claudia Bincoletto. Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development. Journal of Integrative Medicine, 2019, 17(2): 132-140.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3