扶正抑瘤颗粒药物血清诱导小鼠肝癌细胞凋亡及其机制的研究

doi:10.3736/jcim20050409

Abstract

Objective

To study the effects of Fuzheng Yiliu Granule (FZYLG)-medicated serum on apoptosis of liver cancer cells H22 from mice and its mechanism.

Methods

Liver cancer cells H22 from mice were incubated in culture media containing sera from rabbits medicated with different doses of FZYLG. Flow cytometry was used to examine the cell cycle and analyze the apoptotic rate of the H22 cells. The morphological changes of the H22 cells were observed by transmission electron microscope and the apoptosis related proteins Bcl-2 and Bax were examined by streptavidin-biotin peroxidase complex (SABC) method.

Results

FZYLG-medicated serum could influence the cell cycle and stop the proliferation of H22 cells at the G₁/G₀ phase with apoptotic peak being detected. In culture media with FZYLG-medicated sera, the expression of Bcl-2 decreased while that of Bax increased as compared with that in culture medium with non-medicated serum (P<0.05).

Conclusion

FZYLG-medicated serum can induce apoptosis of the liver cancer cells H22 by influencing the cell cycle, down-regulating the expression of Bcl-2 and up-regulating the expression of Bax.

Key words: Fuzheng Yiliu Granules, Apoptosis, cell cycle, Proto-oncogene proteins c-bcl-2, Bax

CLC Number:

R735.7

Jian-xiong Zhao, Wei-dong Cheng, Rui-feng Xu, Yuan-qing Li. Effects of Fuzheng Yiliu Granule-medicated serum on apoptosis of liver cancer cells from mice and its mechanism. Journal of Chinese Integrative Medicine, 2005, 3(4): 278-281.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20050409

http://www.jcimjournal.com/EN/Y2005/V3/I4/278

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References 10

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Culture medium	n	Apoptotic rate	G₀/G₁ phase	S phase	G₂/M phase
With non-medicated serum	8	5.5±1.3	66.5±5.7	23.0±2.8	11.2±1.2
With low-dose FZYLG-medicated serum	8	25.5±4.7^△△	67.2±6.4	23.2±2.4**	8.8±3.7**
With medium-dose FZYLG-medicated serum	8	30.9±4.1^△△	72.8±4.3^△△	19.3±3.9**	7.6±3.1**
With high-dose FZYLG-medicated serum	8	39.6±5.9*^△△	78.6±6.1^△△	16.8±2.1**	3.7±1.3**
With Tianxian Capsule-medicated serum	8	31.1±4.2^△△	70.9±4.1	3.1±1.8	25.9±5.5

Culture medium	Bcl-2					Bax
Culture medium	–	+	++	u	P	–	+	++	u	P
With non-medicated serum	3	3	9			11	1	3
With low-dose FZYLG-medicated serum	4	3	8	103.5	>0.05	10	4	1	110.5	>0.05
With medium-dose FZYLG-medicated serum	5	9	1	58.5	<0.05	5	4	6	70.5	>0.05
With high-dose FZYLG-medicated serum	7	7	1	52.5	<0.05	2	3	10	44.5	<0.05
With Tianxian Capsule-medicated serum	5	3	7	94.5	>0.05	5	5	5	71.0	>0.05

Effects of Fuzheng Yiliu Granule-medicated serum on apoptosis of liver cancer cells from mice and its mechanism

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