Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 278-281.doi: 10.3736/jcim20050409

• Original Experimental Research • Previous Articles     Next Articles

Effects of Fuzheng Yiliu Granule-medicated serum on apoptosis of liver cancer cells from mice and its mechanism

Jian-xiong Zhao,Wei-dong Cheng,Rui-feng Xu,Yuan-qing Li   

  1. Institute of Integrated Traditional Chinese and Western Medicine, Lanzhou Medical College, Lanzhou, Gansu Province 730000, China
  • Received:2004-12-18 Online:2005-07-20 Published:2005-07-20
  • Contact: Jian-xiong Zhao


To study the effects of Fuzheng Yiliu Granule (FZYLG)-medicated serum on apoptosis of liver cancer cells H22 from mice and its mechanism.


Liver cancer cells H22 from mice were incubated in culture media containing sera from rabbits medicated with different doses of FZYLG. Flow cytometry was used to examine the cell cycle and analyze the apoptotic rate of the H22 cells. The morphological changes of the H22 cells were observed by transmission electron microscope and the apoptosis related proteins Bcl-2 and Bax were examined by streptavidin-biotin peroxidase complex (SABC) method.


FZYLG-medicated serum could influence the cell cycle and stop the proliferation of H22 cells at the G1/G0 phase with apoptotic peak being detected. In culture media with FZYLG-medicated sera, the expression of Bcl-2 decreased while that of Bax increased as compared with that in culture medium with non-medicated serum (P<0.05).


FZYLG-medicated serum can induce apoptosis of the liver cancer cells H22 by influencing the cell cycle, down-regulating the expression of Bcl-2 and up-regulating the expression of Bax.

Key words: Fuzheng Yiliu Granules, Apoptosis, cell cycle, Proto-oncogene proteins c-bcl-2, Bax

CLC Number: 

  • R735.7

Tab 1

Effects of different culture media on cell cycle and apoptosis of H22 cells ($\bar{x}$±s, %)"

Culture medium n Apoptotic rate G0/G1 phase S phase G2/M phase
With non-medicated serum 8 5.5±1.3 66.5±5.7 23.0±2.8 11.2±1.2
With low-dose FZYLG-medicated serum 8 25.5±4.7△△ 67.2±6.4 23.2±2.4** 8.8±3.7**
With medium-dose FZYLG-medicated serum 8 30.9±4.1△△ 72.8±4.3△△ 19.3±3.9** 7.6±3.1**
With high-dose FZYLG-medicated serum 8 39.6±5.9*△△ 78.6±6.1△△ 16.8±2.1** 3.7±1.3**
With Tianxian Capsule-medicated serum 8 31.1±4.2△△ 70.9±4.1 3.1±1.8 25.9±5.5

Tab 2

Effects of different culture media on expressions of Bcl-2 and Bax in H22 cells"

Culture medium Bcl-2 Bax
+ ++ u P + ++ u P
With non-medicated serum 3 3 9 11 1 3
With low-dose FZYLG-medicated serum 4 3 8 103.5 >0.05 10 4 1 110.5 >0.05
With medium-dose FZYLG-medicated serum 5 9 1 58.5 <0.05 5 4 6 70.5 >0.05
With high-dose FZYLG-medicated serum 7 7 1 52.5 <0.05 2 3 10 44.5 <0.05
With Tianxian Capsule-medicated serum 5 3 7 94.5 >0.05 5 5 5 71.0 >0.05

Fig 1

Ultrastructure of cells cultured in medium with non-medicated serum examined by an electron microscope (×4000)"

Fig 2

Ultrastructure of cells cultured in medium with medium-dose FZYLG-medicated serum examined by an electron microscope (×4000)"

[1] 戴恩来, 赵健雄, 朱玉真 , 等. 扶正抑瘤汤对肿瘤细胞周期及端粒酶影响的实验研究[J]. 中国中西医结合杂志, 2001,21(10):760-762
doi: 10.7661/CJIM.2001.10.760
[2] 赵健雄, 曲勇, 陈学忠 , 等. 扶正抑瘤颗粒对食管癌、胃癌组织细胞周期及核转录因子的影响[J]. 中国中西医结合杂志, 2003,23(12):908-910
[3] 生秀杰, 宋和存, 何秀琴 . 抗凋亡基因Bcl-2在子宫内膜癌的表达及意义[J]. 中华肿瘤杂志, 1998,20(5):365-366
doi: 10.3760/j.issn:0253-3766.1998.05.014
[4] Alison MR, Sarraf CE . Apoptosis: regulation and relevance to toxicology[J]. Hum Exp Toxicol, 1995,14(3):234-247
doi: 10.1177/096032719501400302 pmid: 7779453
[5] 周俊 . 中药复方——天然组合化学库与多靶作用机理[J]. 中国中西医结合杂志, 1998,18(2):67
[6] Krajewski S, Krajewska M, Shabaik A , et al. Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitor of Bcl-2[J]. Am J Pathol, 1994,145(6):1323-1336
doi: 10.1097/00000433-199412000-00015 pmid: 7992838
[7] 杨雁, 陈敏珠 . 黄芪总苷对肝癌细胞凋亡及wtp53基因表达的影响[J]. 中国药理学通报, 2001,17(4):447-451
[8] 杨铁虹, 卢保华, 贾敏 , 等. 当归多糖对小鼠免疫功能的影响[J]. 中国药理学通报, 2003,19(4):448-451
[9] 袁静, 顾振伦, 周文轩 , 等. 榄香烯诱导人白血病K562细胞凋亡及调控Bcl-2蛋白的表达[J]. 中国药理学报, 1999,20(2):103-106
[10] 唐清秀 . 墓头回对小鼠免疫功能的影响[J]. 中国中医基础医学杂志, 1998,4(4):27-28
[1] Maged Mohamed Maher Abou-Hashem, Dina Mohamed Abo-elmatty, Noha Mostafa Mesbah, Ahmed Mohamed Abd EL-Mawgoud. Induction of sub-G0 arrest and apoptosis by seed extract of Moringa peregrina (Forssk.) Fiori in cervical and prostate cancer cell lines. Journal of Integrative Medicine, 2019, 17(6): 410-422.
[2] Supakanya Kumkarnjana, Rutt Suttisri, Ubonthip Nimmannit, Apirada Sucontphunt, Mattaka Khongkow, Thongchai Koobkokkruad, Nontima Vardhanabhuti. Flavonoids kaempferide and 4,2′-dihydroxy-4′,5′,6′-trimethoxychalcone inhibit mitotic clonal expansion and induce apoptosis during the early phase of adipogenesis in 3T3-L1 cells. Journal of Integrative Medicine, 2019, 17(4): 288-295.
[3] Shriniwas S. Basaiyye, Sanjay Kashyap, Kannan Krishnamurthi, Saravanadevi Sivanesan. Induction of apoptosis in leukemic cells by the alkaloid extract of garden cress (Lepidium sativum L.). Journal of Integrative Medicine, 2019, 17(3): 221-228.
[4] Carlos R. Oliveira, Daniel G. Spindola, Daniel M. Garcia, Adolfo Erustes, Alexandre Bechara, Caroline Palmeira-dos-Santos, Soraya S. Smaili, Gustavo J.S. Pereira, André Hinsberger, Ezequiel P. Viriato, Maria Cristina Marcucci, Alexandra C.H.F. Sawaya, Samantha L. Tomaz, Elaine G. Rodrigues, Claudia Bincoletto. Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development. Journal of Integrative Medicine, 2019, 17(2): 132-140.
[5] Bruno José Martins Da Silva, Sandro Wilson Gomes Pereira, Ana Paula Drummond Rodrigues, José Luiz Martins Do Nascimento, Edilene Oliveira Silva. In vitro antileishmanial effects of Physalis angulata root extract on Leishmania infantum. Journal of Integrative Medicine, 2018, 16(6): 404-410.
[6] Eric Wei Chiang Chan, Siu Kuin Wong, Hung Tuck Chan . Casticin from Vitex species: A short review on its anticancer and anti-inflammatory properties. Journal of Integrative Medicine, 2018, 16(3): 147-152.
[7] Jesmin Mondal, Asmita Samadder, Anisur Rahman Khuda-Bukhsh. Psorinum 6× triggers apoptosis signals in human lung cancer cells. Journal of Integrative Medicine, 2016, 14(2): 143-153.
[8] Qing-qing Zang, Lu Zhang, Ning Gao, Cheng Huang. Ophiopogonin D inhibits cell proliferation, causes cell cycle arrest at G2/M, and induces apoptosis in human breast carcinoma MCF-7 cells. Journal of Integrative Medicine, 2016, 14(1): 51-59.
[9] Santu Kumar Saha, Sourav Roy, Anisur Rahman Khuda-Bukhsh. Ultra-highly diluted plant extracts of Hydrastis canadensis and Marsdenia condurango induce epigenetic modifications and alter gene expression profiles in HeLa cells in vitro. Journal of Integrative Medicine, 2015, 13(6): 400-411.
[10] Samrat Ghosh, Sourav Sikdar, Avinaba Mukherjee, Anisur Rahman Khuda-Bukhsh. Evaluation of chemopreventive potentials of ethanolic extract of Ruta graveolens against A375 skin melanoma cells in vitro and induced skin cancer in mice in vivo. Journal of Integrative Medicine, 2015, 13(1): 34-44.
[11] Samrat Ghosh, Kausik Bishayee, Anisur Rahman Khuda-Bukhsh​. Oleanolic acid isolated from ethanolic extract of Phytolacca decandra induces apoptosis in A375 skin melanoma cells: Drug-DNA interaction and signaling cascade. Journal of Integrative Medicine, 2014, 12(2): 102-114.
[12] Paul Avijit, Das Sreemanti, Das Jayeeta, Samadder Asmita, Bishayee Kausik, Sadhukhan Ratan, Anisur Rahman Khuda-Bukhsh. Diarylheptanoid-myricanone isolated from ethanolic extract of Myrica cerifera shows anticancer effects on HeLa and PC3 cell lines: Signalling pathway and drug-DNA interaction. Journal of Chinese Integrative Medicine, 2013, 11(6): 405-415.
[13] Farha A. Kabeer, Geetha B. Sreedevi, Mangalam S. Nair, Dhanya S. Rajalekshmi, Latha P. Gopalakrishnan, Sujathan Kunjuraman, Remani Prathapan. Antineoplastic effects of deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, on lung adenocarcinoma (A549) cells. Journal of Chinese Integrative Medicine, 2013, 11(4): 269-277.
[14] Samrat Ghosh, Kausik Bishayee, Avijit Paul, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Homeopathic mother tincture of Phytolacca decandra induces apoptosis in skin melanoma cells by activating caspase-mediated signaling via reactive oxygen species elevation. Journal of Chinese Integrative Medicine, 2013, 11(2): 116-124.
[15] Avijit Paul, Kausik Bishayee, Samrat Ghosh, Avinaba Mukherjee, Sourav Sikdar, Debrup Chakraborty, Naoual Boujedaini, Anisur Rahman Khuda-Bukhsh. Chelidonine isolated from ethanolic extract of Chelidonium majus promotes apoptosis in HeLa cells through p38-p53 and PI3K/AKT signalling pathways. Journal of Chinese Integrative Medicine, 2012, 10(9): 1025-1038.
Full text



[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[5] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[6] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[7] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[8] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[9] A-gao Zhou, Yong Zhang, Gang Kui, De-Yun Kong, Hai-liang Ge, Qiu-hua Ren, Jia-rong Dong, Sheng Hong, Xu-ming Mao, Yin Wang, Hui-zheng Zhang, Shu-jun Wang. Influence of traditional Chinese compound recipes with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 77-82
[10] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14