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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 278-281.doi: 10.3736/jcim20050409

• Original Experimental Research • Previous Articles     Next Articles

Effects of Fuzheng Yiliu Granule-medicated serum on apoptosis of liver cancer cells from mice and its mechanism

Jian-xiong Zhao,Wei-dong Cheng,Rui-feng Xu,Yuan-qing Li   

  1. Institute of Integrated Traditional Chinese and Western Medicine, Lanzhou Medical College, Lanzhou, Gansu Province 730000, China
  • Received:2004-12-18 Online:2005-07-20 Published:2005-07-20
  • Contact: Jian-xiong Zhao E-mail:ZhaoJX-01@126.com

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Objective

To study the effects of Fuzheng Yiliu Granule (FZYLG)-medicated serum on apoptosis of liver cancer cells H22 from mice and its mechanism.

Methods

Liver cancer cells H22 from mice were incubated in culture media containing sera from rabbits medicated with different doses of FZYLG. Flow cytometry was used to examine the cell cycle and analyze the apoptotic rate of the H22 cells. The morphological changes of the H22 cells were observed by transmission electron microscope and the apoptosis related proteins Bcl-2 and Bax were examined by streptavidin-biotin peroxidase complex (SABC) method.

Results

FZYLG-medicated serum could influence the cell cycle and stop the proliferation of H22 cells at the G1/G0 phase with apoptotic peak being detected. In culture media with FZYLG-medicated sera, the expression of Bcl-2 decreased while that of Bax increased as compared with that in culture medium with non-medicated serum (P<0.05).

Conclusion

FZYLG-medicated serum can induce apoptosis of the liver cancer cells H22 by influencing the cell cycle, down-regulating the expression of Bcl-2 and up-regulating the expression of Bax.

Key words: Fuzheng Yiliu Granules, Apoptosis, cell cycle, Proto-oncogene proteins c-bcl-2, Bax

CLC Number: 

  • R735.7

Tab 1

Effects of different culture media on cell cycle and apoptosis of H22 cells （$\bar{x}$±s, %）"

Culture medium n Apoptotic rate G0/G1 phase S phase G2/M phase
With non-medicated serum 8 5.5±1.3 66.5±5.7 23.0±2.8 11.2±1.2
With low-dose FZYLG-medicated serum 8 25.5±4.7△△ 67.2±6.4 23.2±2.4** 8.8±3.7**
With medium-dose FZYLG-medicated serum 8 30.9±4.1△△ 72.8±4.3△△ 19.3±3.9** 7.6±3.1**
With high-dose FZYLG-medicated serum 8 39.6±5.9*△△ 78.6±6.1△△ 16.8±2.1** 3.7±1.3**
With Tianxian Capsule-medicated serum 8 31.1±4.2△△ 70.9±4.1 3.1±1.8 25.9±5.5

Tab 2

Effects of different culture media on expressions of Bcl-2 and Bax in H22 cells"

Culture medium Bcl-2 Bax
– + ++ u P – + ++ u P
With non-medicated serum 3 3 9 11 1 3
With low-dose FZYLG-medicated serum 4 3 8 103.5 >0.05 10 4 1 110.5 >0.05
With medium-dose FZYLG-medicated serum 5 9 1 58.5 <0.05 5 4 6 70.5 >0.05
With high-dose FZYLG-medicated serum 7 7 1 52.5 <0.05 2 3 10 44.5 <0.05
With Tianxian Capsule-medicated serum 5 3 7 94.5 >0.05 5 5 5 71.0 >0.05

Fig 1

Ultrastructure of cells cultured in medium with non-medicated serum examined by an electron microscope （×4000）"

Fig 2

Ultrastructure of cells cultured in medium with medium-dose FZYLG-medicated serum examined by an electron microscope （×4000）"

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