Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (6): 455-458.doi: 10.3736/jcim20050609

• Original Clinical Research • Previous Articles     Next Articles

Effects of Liuwei Dihuang Pills on expressions of apoptosis-related genes bcl-2 and Bax in pancreas of OLETF rats

Yao-ming Xue1,Ren Luo2,Bo Zhu1,Yan Zhang1,Yong-hua Pan1,Chen-zhong Li1   

  1. 1. Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province 510515, China
    2. Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province 510515, China
  • Received:2005-08-15 Online:2005-11-20 Published:2005-11-20
  • Contact: Yao-Ming XUE E-mail:brightxue@163.com

Objective

To investigate the effects of Liuwei Dihuang Pills (LWDHP) on expressions of apoptosis-related genes bcl-2 and Bax in pancreas of OLETF rats.

Methods

Forty male OLETF rats were randomly divided into LWDHP-treated group and untreated group. Another ten male LETO rats were included in normal control group. OLETF rats in the LWDHP-treated group were given LWDHP (2.4 g·kg -1·d -1) orally since the age of 8 weeks and the rats in the other two groups were given distilled water orally. Body weights of rats were recorded weekly and blood glucose concentration was determined by oral glucose tolerance test (OGTT). Pancreas weights were recorded after rats were killed and the expression levels of bcl-2 mRNA and Bax mRNA were detected by reverse transcriptase-polymerase chain reaction (RT-PCR).

Results

In the LWDHP-treated group, the expression of bcl-2 mRNA in the pancreas of rats at the age of 40 weeks (1.25±0.07) was much higher than that in the untreated group (1.01±0.16), P<0.01. Bax mRNA level in the LWDHP-treated group (0.57±0.11) was obviously lower than that in the untreated group (1.18±0.28), P<0.01. There was no significant difference of pancreas-to-body weight ratios between the LWDHP-treated group and the untreated group. The ability of glucose tolerance was improved in the LWDHP-treated group.

Conclusion

LWDHP can up-regulate the expression of bcl-2 and down-regulate the expression of Bax at transcription level, which maybe contribute to the anti-apoptosis effects of LWDHP.

Key words: Diabetes mellitus, Type 2, Apoptosis, Gene bcl-2, Gene Bax, Liuwei Dihuang Decoction, Rats, OLETF

CLC Number: 

  • R587.1

Tab 1

Blood glucose concentration and AUC of rats in 3 groups ($\bar{x}$±s )"

Group n PBG 2 h
(mmol/L)
AUC
(mmol/L)
Normal control group (LETO)
at age of 30 weeks 7 7.5±0.9 28.8±1.7
at age of 34 weeks 4 8.6±1.2 30.6±1.7
at age of 40 weeks 4 7.9±1.2 29.8±0.6
Untreated group (OLETF)
at age of 30 weeks 17 10.5±1.9 46.5±4.4△△
at age of 34 weeks 14 13.7±4.2 57.6±10.2△△
at age of 40 weeks 14 14.8±3.2 63.4±8.9△△
LWDHP-treated
at age of 30 weeks 20 9.4±2.3 40.4±10.3*△△
at age of 34 weeks 14 11.6±3.1 47.4±11.1*△△
at age of 40 weeks 14 12.0±3.0** 54.0±10.9*△△

Tab 2

Pancreas-to-body weight ratio of rats in 3 groups ($\bar{x}$±s , %)"

Group n Pancreas-to-
body weight ratio
Normal control group (LETO)
at age of 8 weeks 3 0.17±0.02
at age of 32 weeks 3 0.20±0.01
at age of 40 weeks 4 0.19±0.02
Untreated group (OLETF)
at age of 8 weeks 3 0.16±0.04
at age of 32 weeks 3 0.15±0.06
at age of 40 weeks 3 0.14±0.02**
LWDHP-treated
at age of 32 weeks 5 0.19±0.02
at age of 40 weeks 7 0.16±0.01**

Tab 3

Expression levels of bcl-2 and Bax mRNAs of rats in 3 groups ($\bar{x}$±s )"

Group n bcl-2 mRNA Bax mRNA
Normal control group (LETO)
at age of 8 weeks 3 1.48 0.19 0.26 0.05
at age of 32 weeks 3 1.42±0.20 0.28±0.12**
at age of 40 weeks 4 1.33±0.11** 0.41±0.08**
Untreated group (OLETF)
at age of 8 weeks 3 1.36±0.22 0.23±0.04
at age of 32 weeks 3 1.11±0.06 1.31±0.10
at age of 40 weeks 3 1.01±0.16 1.18±0.28
LWDHP-treated
at age of 32 weeks 5 1.29±0.17 0.28±0.17**
at age of 40 weeks 7 1.25±0.07** 0.57±0.11**
[1] 张汝学, 周金黄, 张永祥 , 等. 去胸腺对大鼠糖代谢的影响及地黄寡糖对其的调节作用[J]. 中国药理学通报, 2002,18(2):194-197
[2] 王敏 . 六味地黄丸合用消渴丸对糖尿病大鼠血糖及血脂代谢的实验研究[J]. 中华实用中西医杂志, 2003,3(16):1086-1088
[3] Butler AE, Janson J, Bonner-Weir S , et al. Beta-cell deficit and increased beta-cell apoptosis in humans with type 2 diabetes[J]. Diabetes, 2003,52(1):102-110
doi: 10.2337/diabetes.52.1.102
[4] Butler AE, Janson J, Ritzel R , et al. Accelerated apoptosis overcomes increased replication to cause β-cell loss in diabetes in mice transgenic for h-IAPP[J]. Diabetes, 2002,51(Suppl 2):A7
doi: 10.2337/diabetes.51.1.7
[5] 胡合营, 郑军萍 . 糖尿病患者血中SOD含量变化与维生素E、六味地黄丸关系的临床观察[J]. 放射免疫学杂志, 2001,14(3):140-141
[6] Maechler P, Jornot L, Wollheim CB . Hydrogen peroxide alters mitochondrial activation and insulin secretion in pancreatic beta cells[J]. J Biol Chem, 1999,274(39):27905-27913
doi: 10.1074/jbc.274.39.27905
[7] Kawano K, Hirashima T, Mori S , et al. Spontaneous long-term hyperglycemic rat with diabetic complications. Otsuka Long-Evans Tokushima Fatty(OLETF) strain[J]. Diabetes, 1992,41(11):1422-1428
doi: 10.2337/diab.41.11.1422
[1] Theocharis Koufakis, Kalliopi Kotsa, Nikolaos Papanas. Remission of type 2 diabetes depends on prompt comprehensive lifestyle changes upon diagnosis: How can this “Road to Damascus” experience be supported?. Journal of Integrative Medicine, 2022, 20(4): 288-291.
[2] Cai-lian Fana, Wan-jun Caib, Meng-nan Ye, Miao Chen, Yi Dai. Qili Qiangxin, a compound herbal medicine formula, alleviates hypoxia-reoxygenation-induced apoptotic and autophagic cell death via suppression of ROS/AMPK/mTOR pathway in vitro. Journal of Integrative Medicine, 2022, 20(4): 365-375.
[3] Hong-xiao Li, Ling Shi, Shang-jie Liang Chen-chen Fang, Qian-qian Xu, Ge Lu, Qian Wang, Jie Cheng, Jie Shen, Mei-hong Shen. Moxibustion alleviates decreased ovarian reserve in rats by restoring the PI3K/AKT signaling pathway. Journal of Integrative Medicine, 2022, 20(2): 163-172.
[4] Mohamed S. Othman, Sofian T. Obeidat, Amal H. Al-Bagawi, Mohamed A. Fareid, Alaa Fehaid, Ahmed E. Abdel Moneim. Green-synthetized selenium nanoparticles using berberine as a promising anticancer agent#br#
#br#
. Journal of Integrative Medicine, 2022, 20(1): 65-72.
[5] Hyongjun Jeon, Hee-Young Kim, Chang-Hwan Bae, Yukyung Lee, Sungtae Koo, Seungtae Kim. Korean red ginseng decreases 1-methyl-4-phenylpyridinium-induced mitophagy in SH-SY5Y cells. Journal of Integrative Medicine, 2021, 19(6): 537-544.
[6] Yunita Sari, Akhyarul Anam, Annas Sumeru, Eman Sutrisna. The knowledge, attitude, practice and predictors of complementary and alternative medicine use among type 2 diabetes mellitus patients in Indonesia. Journal of Integrative Medicine, 2021, 19(4): 347-353.
[7] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[8] Pukar Khanal, Basanagouda M. Patil. Integration of network and experimental pharmacology to decipher the antidiabetic action of Duranta repens L.. Journal of Integrative Medicine, 2021, 19(1): 66-77.
[9] Baoyi Shao, Saiying Hou, Yuenyan Chan, Changchun Shao, Lixing Lao. Remission of new-onset type 2 diabetes mellitus in an adolescent using an integrative medicine approach: A case report. Journal of Integrative Medicine, 2021, 19(1): 85-88.
[10] Abdulhakim Abubakar, Abdullahi Balarabe Nazifi, Idris Mohammed Maje, Yusuf Tanko, Joseph Akpojo Anuka, Ezzeldin Mukthar Abdurahman. Antihyperglycaemic activity of ethylacetate extract of Chlorophytum alismifolium in type 2 diabetes: The involvement of peroxisome proliferator-activated receptor-γ and dipeptidyl peptidase-4. Journal of Integrative Medicine, 2021, 19(1): 78-84.
[11] Porwornwisit Tritripmongkol, Tullayakorn Plengsuriyakarn, Mayuri Tarasuk, Kesara Na-Bangchang. In vitro cytotoxic and toxicological activities of ethanolic extract of Kaempferia galanga Linn. and its active component, ethyl-p-methoxycinnamate, against cholangiocarcinoma. Journal of Integrative Medicine, 2020, 18(4): 326-333.
[12] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[13] Varuni Colamba Pathiranage, Ira Thabrew, Sameera R. Samarakoon, Kamani H. Tennekoon, Umapriyatharshini Rajagopalan, Meran K. Ediriweera. Evaluation of anticancer effects of a pharmaceutically viable extract of a traditional polyherbal mixture against non-small-cell lung cancer cells. Journal of Integrative Medicine, 2020, 18(3): 242-252.
[14] Mohadeseh Ostovar, Abolfazl Akbari, Mohammad Hossein Anbardar, Aida Iraji, Mohsen Salmanpour, Salar Hafez Ghoran, Mojtaba Heydari, Mesbah Shams. Effects of Citrullus colocynthis L. in a rat model of diabetic neuropathy. Journal of Integrative Medicine, 2020, 18(1): 59-67.
[15] Maged Mohamed Maher Abou-Hashem, Dina Mohamed Abo-elmatty, Noha Mostafa Mesbah, Ahmed Mohamed Abd EL-Mawgoud. Induction of sub-G0 arrest and apoptosis by seed extract of Moringa peregrina (Forssk.) Fiori in cervical and prostate cancer cell lines. Journal of Integrative Medicine, 2019, 17(6): 410-422.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278