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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (1): 52-55.doi: 10.3736/jcim20060114

• Original Experimental Research • Previous Articles     Next Articles

Arnebia root oil promotes wound healing and expression of basic fibroblast growth factor on the wound surface in rabbits

Xian-wu Pei1,2, Kun-zheng Wang1, Xiao-qian Dang1, Jin-hui Song1, Zhi-bin Shi1, Deng-feng Gao1   

  1. 1. Department of Orthopedics, Second Hospital, Xi’an Jiaotong University, Xi’an, Shaanxi Province 710004, China;
    2. Department of Orthopedics, Taizhou Central Hospital, Taizhou, Zhejiang Province 318000, China
  • Online:2006-01-20 Published:2018-10-25

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Objective

To observe the promoting effect of arnebia root oils on expression of basic fibroblast growth factor (bFGF) in skin wound of rabbits and the histomorphological changes in the wound surface, and to discuss its mechanism.

Methods

Bilateral round skin wounds were made on the back of 15 rabbits. The three wounds on one side of the back of each rabbit were treated with arnebia root oils, while the three wounds on the other side were treated with vaseline in order to promote the wound healing. The histomorphology and ultrastructure under electron microscopy of the wounds,and the rate of wound healing were examined at different time. Western blotting assay was used to detect the expression of bFGF in the wound surface. 

Results

The healing rate of the arnebia root oils-treated wounds was evidently higher than that of the vaseline-treated wounds (P＜0.05). The quantities of fibroblast, collagen and capillary in the arnebia root oils-treated wounds were much more than those in the vaseline-treated wounds, and the expression of endogenous bFGF in the arnebia root oils-treated wounds was enhanced obviously as compared with that in the vaseline-treated wounds in different period of wound healing. There existed a parallel correlation between the expression level of bFGF and the rate of wound healing. 

Conclusion

The promoting effect of arnebia root oils on wound healing may be related to increasing the expression level of basic fibroblast growth factor in the skin wound.

Key words: Arnebia root oils, Basic fibroblast growth factor, Surface of wound, Histomorphology

CLC Number: 

  • R264

Tab 1

Comparisons of wound healing rate at different time between two treatments（ヌ±S）"

Healing rate at different time after skin wound
Skin wound
n
3rd day7th day14th day21st day
Arnebia root oils-treated450.16±0.15*0.34±0.12*0.29±0.22*0.26±0.14*
Vaseline-treated450.13±0.090.30±0.130.28±0.060.20±0.15

Figure 1

Histomorphology of arnebia root oils-treated wound surface at the 7th day after skin wound （HE staining, ×100）"

Figure 2

Histomorphology of arnebia root oils-treated wound surface at the 14th day after skin wound （HE staining, ×40）"

Figure 3

Histomorphology of arnebia root oils-treated wound surface at the 21st day after skin wound （HE staining, ×100）"

Figure 4

Changes of expression level of bFGF at different time of wound healingA: Arnebia root oils-treated wounds; B: Vaseline-treated wounds; A3, A7, A14, A21: Arnebia root oils-treated wounds at 3rd, 7th, 14th, and 21st day of wound healing; B3, B7, B14, B21: Vaseline-treated wounds at 3rd, 7th, 14th and 21st day; *P<0.05, **P<0.01, vs vaseline-treated wounds."

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