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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (6): 589-595.doi: 10.3736/jcim20060609

• Original Experimental Research • Previous Articles     Next Articles

Physiological and biochemical effects of intermittent fasting combined with hunger-resistant food on mice

Xiao-dong Shen,Wei-guo Hua,Wei-zhong Chu,Feng Xu, Yu-ying Wang, Hui-ju Chen   

  1. Shanghai Qigong Institute, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China
  • Online:2006-11-30 Published:2006-11-15


To observe the physiological and biochemical effects of intermittent fasting combined with hunger-resistant food on mice, and to evaluate the safety and beneficial effects of this regimen.


One hundred and forty-four adult ICR mice were divided into 4 groups: standard feed AL group (ad libitum intake of standard feed), hunger-resistant food AL group (ad libitum intake of hunger-resistant food), standard feed IF group (feeding standard feed and fasting on alternate days), and hunger-resistant food IF group (feeding hunger-resistant food and fasting on alternate days). The experiment lasted for 4-8 weeks and all mice drank water freely. The quality of life, body weight, fasting blood glucose, serum lipid, blood routine test, liver and kidney functions as well as the viscera indexes were examined.


Compared to the standard feed AL group, the caloric taking and the increment of body-weight were reduced (P<0.01), and the viscera indexes of the liver and kidney were elevated (P<0.05) in the hunger-resistant food AL group and the hunger-resistant food IF group, the values of fasting blood glucose were reduced in standard feed IF group and hunger-resistant food IF group (P<0.01), the value of triglycerides was reduced in hunger-resistant food IF group (P<0.05), while the quality of life, blood routine test as well as the liver and kidney functions were not obviously affected in the hunger-resistant food AL group, standard feed IF group and hunger-resistant food IF group.


The regimen of intermittent fasting combined with hunger-resistant food is safe and beneficial to metabolic regulation, such as controlling body-weight and adjusting blood glucose and serum lipid. It is expected that development of this regimen will be helpful to the control of obesity and diabetes, etc.

Key words: Fasting, Body-weight, Blood glucose, Triglycerides, mice

CLC Number: 

  • R363

Table 1

Nutrition comparison of standard feed with hunger-resistant food"

(g/100 g)
(g/100 g)
(kJ/100 g)
Standard feed204.01 4733.36
Hunger-resistant food3318.51 8703.69

Table 2

Food-taking and caloric taking of mice in 4 groups during 8-week experiment (ヌ±S)"

GroupnFood-taking (g/d)Caloric taking (kJ/d)
Standard feed AL175.94±0.3187.32±4.59
Hunger-resistant food AL184.23±0.17**△△79.35±3.28**
Standard feed IF245.55±0.34**81.65±4.97**
Hunger-resistant food IF273.63±0.25**△△▲▲67.87±4.87**△△▲▲

Table 3

Body-weight change of mice in 4 groups during 8-week experiment (ヌ±S, g)"

GroupnBody-weight at different timeBody-weight
0 week2 weeks4 weeks6 weeks8 weeksincrement
Standard feed AL1731.50±0.9640.33±2.2441.44±2.5741.88±2.7540.06±2.508.56±2.16
Hunger-resistant food AL1831.24±1.8739.54±2.5640.47±2.5440.00±2.6037.64±2.88*6.41±2.48**
Standard feed IF2430.67±2.0634.06±2.4734.98±3.4136.34±3.0138.63±3.297.95±2.35
Hunger-resistant food IF2731.36±2.2533.18±2.2034.76±2.6335.16±1.4237.51±2.99**6.14±1.99**△△

Table 4

Viscera index of mice in 4 groups after 8-week experiment (ヌ±S, %)"

GroupnLiverKidneySpleenStomachTesticleThyroid gland
Standard feed AL174.01±0.251.88±0.222.37±0.321.56±0.447.14±0.935.96±0.60
Hunger-resistant food AL184.57±0.24**△△2.01±0.16△△2.46±0.461.51±0.457.16±0.736.42±0.68*
Standard feed IF244.14±0.281.74±0.19*2.24±0.391.56±0.337.00±0.816.64±0.70**
Hunger-resistant food IF274.43±0.35**△△1.90±0.22△△2.47±0.451.61±0.337.07±0.856.80±0.69**

Table 5

Fasting blood glucose of mice in 4 groups after 8-week experiment (ヌ±S, mmol/L)"

Standard feed AL178.50±1.47
Hunger-resistant food AL188.20±1.10
Standard feed IF246.00±1.36**△△
Hunger-resistant food IF276.20±0.84**△△

Table 6

Serum lipid of mice in 3 groups after 4-week experiment (ヌ±S, mmol/L)"

Standard feed AL62.30±0.181.29±0.09
Standard feed IF62.26±0.181.26±0.09
Hunger-resistant food IF62.82±0.721.00±0.29*
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