蒲黄总黄酮对3T3-L1脂肪细胞糖脂代谢的影响

doi:10.3736/jcim20060610

Abstract

Objective

To observe the effects of Pollen Typhae total flavone (PTF) on glucose and lipid metabolism in 3T3-L1 adipocytes.

Methods

The content of glucose which disappeared from the culture medium after incubation with drugs for 24 hours was determined as glucose consumption of the cells. The activity of cells was detected by XTT method. The transport of glucose was observed by ³H-glucose uptake method. The efflux of free fatty acid (FFA) from adipocytes was observed by the concentration of FFA in the culture medium.

Results

The glucose concentration in culture medium was significantly decreased with a concentration-dependent effect, when PTF concentrations were from 0.025 g/L to 0.4 g/L. The toxic effect on cells appeared while PTF concentration was 0.4 g/L, and the MTT value decreased. PTF also significantly increased glucose transportation in the 3T3-L1 adipocytes as rosiglitazone (ROS) did. At the same time, FFA concentration in culture medium was significantly decreased as compared to the normal control group, while ROS-treated group did not show any difference.

Conclusion

PTF can increase insulin sensitivity by increasing glucose transportation and consumption in the 3T3-L1 adipocytes as well as decreasing the FFA efflux from the cells.

Key words: Pollen Typhae, Flavone, Glucose, Free fatty acid

CLC Number:

R285

Yan-ming He, Wen-jian Wang, Wei-hua Chen, Jian Ying, Chun-yan He, Yu-yin Ma, Yi Liu, You-shen Li. Effects of Pollen Typhae total flavone on glucose and lipid metabolism in 3T3-L1 adipocytes. Journal of Chinese Integrative Medicine, 2006, 4(6): 593-595.

/ / Recommend

Add to citation manager EndNote| Reference Manager|ProCite|BibTeX|RefWorks

URL: http://www.jcimjournal.com/EN/10.3736/jcim20060610

http://www.jcimjournal.com/EN/Y2006/V4/I6/593

TrendMD

Figures/Tables 4

Table 1

Table 2

Figure 1

Figure 2

References 7

[1]	Barnes DM, Kircher EA . Effects of mercuric chloride on glucose transport in 3T3-L1 adipocytes[J]. Toxicol In Vitro, 2005,19(2):207-214 doi: 10.1016/j.tiv.2004.08.005 pmid: 15649634
[2]	Maier VH, Gould GW . Long-term insulin treatment of 3T3-L1 adipocytes results in mis-targeting of GLUT4: implications for insulin-stimulated glucose transport[J]. Diabetologia, 2000,43(10):1273-1281
[3]	Williams C, Espinosa OA, Montenegro H , et al. Hydrosoluble formazan XTT: its application to natural products drug discovery for Leishmania[J]. J Microbiol Methods, 2003,55(3):813-816 doi: 10.1016/j.mimet.2003.08.013 pmid: 1460742612
[4]	Romero R, Casanova B, Pulido N , et al. Stimulation of glucose transport by thyroid hormone in 3T3-L1 adipocytes: increased abundance of GLUT1 and GLUT4 glucose transporter proteins[J]. J Endocrinol, 2000,164(2):187-195
[5]	王海波, 王章元 . 蒲黄药理作用的研究进展[J]. 医药导报, 2005,24(4):318-319
[6]	Shepherd PR . Mechanisms regulating phosphoinositide 3-kinase signaling in insulin-sensitive tissues[J]. Acta Physiol Scand, 2005,183(1):3-12
[7]	Belfort R, Mandarino L, Kashyap S , et al. Dose-response effect of elevated plasma free fatty acid on insulin signaling[J]. Diabetes, 2005,54(6):1640-1648 doi: 10.2337/diabetes.54.6.1640 pmid: 15919784

Related Articles 15

[1]	Esmaeil Yousefi Rad, Behzad Nazarian, Somayeh Saboori, Ebrahim Falahi, Azita Hekmatdoost. Effects of L-arginine supplementation on glycemic profile: Evidence from a systematic review and meta-analysis of clinical trials. Journal of Integrative Medicine, 2020, 18(4): 284-291.
[2]	Eric Wei Chiang Chan, Siu Kuin Wong, Hung Tuck Chan . Casticin from Vitex species: A short review on its anticancer and anti-inflammatory properties. Journal of Integrative Medicine, 2018, 16(3): 147-152.
[3]	Nazli Samadi, Hassan Mozaffari-Khosravi, Masoud Rahmanian, Mohsen Askarishahi. Effects of bee propolis supplementation on glycemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: A randomized, double-blind clinical trial. Journal of Integrative Medicine, 2017, 15(2): 124-134.
[4]	Samadder Asmita, Das Jayeeta, Das Sreemanti, Biswas Raktim, Rahman Khuda-Bukhsh Anisur. Ameliorative potentials of Syzygium jambolanum extract against arsenic-induced stress in L6 cells in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1293-1302.
[5]	Anisur Rahman Khuda-Bukhsh, Arnab De, Durba Das, Suman Dutta, Naoual Boujedaini. Analysis of the capability of ultra-highly diluted glucose to increase glucose uptake in arsenite-stressed bacteria Escherichia coli. Journal of Chinese Integrative Medicine, 2011, 9(8): 901-912.
[6]	Zhao Li, Liang Xiao-chun, Zhang Hong, Wang Pu-yan, Sun Qing, Huang Wen-zhi, Li Bo-wu. Effects of Chinese herbal medicine Jinmaitong-medicated serum on inducible nitric oxide synthase and NADPH oxidase p22-phox subunit of rat Schwann cells cultured in high-glucose medium. Journal of Chinese Integrative Medicine, 2011, 9(3): 299-305.
[7]	Lei Chen, Xiao-ying Bi, Li-xun Zhu, Yi-qing Qiu, Su-ju Ding, Ben-qiang Deng. Flavonoids of puerarin versus tanshinone ⅡA for ischemic stroke: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2011, 9(11): 1215-1220.
[8]	Hong-li Xue, Zeng Zhang, Wen-jian Wang, Wei-hua Chen, Jian Ying. Effects of Chinese herbal medicine Yiqi Zengmin Formula on expression of glucose transporter 4 in skeletal muscle in type 2 diabetic rats. Journal of Chinese Integrative Medicine, 2011, 9(10): 1133-1137.
[9]	Xiang-yu Guo, Ying Duan, Juan-e Li , Li-xia Yang, Lian-sha Huang, Zhi-cheng Wang, He-yao Wang, Tong-hua Liu. Antidiabetic effects of Tangnaikang on obese Zucker rats and the mechanism. Journal of Chinese Integrative Medicine, 2010, 8(6): 535-540.
[10]	Hong-shan Li, Qin Feng, Li-li Xu, Shao-dong Chen, Xue-mei Li, Yi-yang Hu. Effects of Qushi Huayu Decoction in prevention and treatment of fatty liver in rats based on adiponection-free fatty acid pathway. Journal of Chinese Integrative Medicine, 2009, 7(6): 546-551.
[11]	Bang-jiang Fang, Shuang Zhou, Xin-jun Pei, Jin-yang Huang, Bao-jin Chen, Yun Geng , Li-kun Yang. Effects of Yanggan Lidan Granule on insulin resistance in guinea pigs with induced cholesterol gallstones. Journal of Chinese Integrative Medicine, 2009, 7(12): 1159-1163.
[12]	Gui-song Zhu, Jin-song He. Effects of ligustrazine injection on high glucose-induced type Ⅰ collagen, matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 expressions in human peritoneal mesothelial cells in vitro. Journal of Chinese Integrative Medicine, 2009, 7(1): 65-69.
[13]	Hui Zhang, Qin Feng, Hong-shan Li, Shao-dong Chen, Xiao-ning Wang, Jing-hua Peng, Ning Zhang, Yi-yang Hu. Effects of Qushi Huayu Decoction on cathepsin B and tumor necrosis factor-α expression in rats with non-alcoholic steatohepatitis. Journal of Chinese Integrative Medicine, 2008, 6(9): 928-933.
[14]	Yan-ming He, Wen-jian Wang, Wua-wei Chen, Jian Ying. Effects of Pollen Typhae total flavones on mRNA expressions of peroxisome proliferator-activated receptors in 3T3-L1 adipocytes. Journal of Chinese Integrative Medicine, 2008, 6(9): 939-941.
[15]	Shao-Ying Lou, Yi Liu, Wei-Hua Chen, Jian Ying, Yan-Ming He, Wen-jian Wang. Pollen Typhae total flavones inhibit expression of interleukin-6 in C2C12 skeletal muscle cells cultured with palmitate. Journal of Chinese Integrative Medicine, 2008, 6(5): 488-491.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 10

[1]	Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[2]	Hao Li, Ming-jiang Yao, Wen-ming Zhao, Jie Guan, Lin-lin Cai, Ling Cui. A randomized, controlled, double-blind trial of Huannao Yicong capsule in senile patients with mild cognitive impairment. Journal of Chinese Integrative Medicine, 2008, 6(1): 25-31
[3]	Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[4]	SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[5]	Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6]	Ning-qun Wang, Liang-duo Jiang, Zong-xing Li. Research progress in asthma-related quality of life. Journal of Chinese Integrative Medicine, 2008, 6(1): 93-97
[7]	Jing-yuan Mao, Chang-xiao Liu, Heng-he Wang, Guang-li Wei , Zhen-peng Zhang, Jie Xing, Wang Xian liang , Ying-fei Bi . Effects of Shenmai Injection on serum concentration and pharmacokinetics of digoxin in dogs with heart failure. Journal of Chinese Integrative Medicine, 2010, 8(11): 1070-1074
[8]	Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100
[9]	Gui Yu, Jie Wang. Thinking on building the network cardiovasology of Chinese medicine. Journal of Chinese Integrative Medicine, 2012, 10(11): 1206-1210
[10]	Pedro Saganha João, Doenitz Christoph, Greten Tobias, Efferth Thomas, J. Greten Henry. Qigong therapy for physiotherapists suffering from burnout: a preliminary study. Journal of Chinese Integrative Medicine, 2012, 10(11): 1233-1239

Group	n	Glucose consumption
Normal control	8	3.06±0.11
Ros-treated (5 μmol/L)	8	9.60±0.42**
PTF-treated
0.025 g/L	8	3.12±0.26
0.05 g/L	8	3.54±0.40**
0.1 g/L	8	4.58±0.42**
0.2 g/L	8	5.08±0.26**
0.4 g/L	8	5.54±0.83**

Group	n	XTT OD value (x±s)	XTT rate
Normal control	8	2.60±0.14	1.00
Ros-treated (5 μmol/L)	8	2.68±0.09	1.03
PTF-treated
0.025 g/L	8	2.66±0.08	1.02
0.05 g/L	8	2.63±0.07	1.01
0.1 g/L	8	2.79±0.06	1.07
0.2 g/L	8	2.73±0.04	1.05
0.4 g/L	8	1.77±0.23**	0.68

Effects of Pollen Typhae total flavone on glucose and lipid metabolism in 3T3-L1 adipocytes

RichHTML

PDF (PC)

Like