Lieber-DeCarli酒精性肝损伤模型的复制

doi:10.3736/jcim20060611

Abstract

Objective

Duplicating the classical alcoholic hepatic injury model, to provide an ideal animal model for research on prevention and treatment of hepatic injury.

Methods

According to the prescription of Lieber-DeCarli, the same calorie fluid animal feed, which contained ethanol or non-ethanol, was prepared. Twenty-three rats were divided into normal control group (n=5), control liquid diet group (n=9), ethanol liquid diet group (n=9). Rats in the normal control group were fed normal diet, and rats in the control liquid diet group and ethanol liquid diet group were fed the corresponding diet for eight weeks. The pathologic changes of hepatic tissue were observed. The activities of the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the liver tissue γ-glutamyltransferase (γ-GT), the content of triglyceride (TG), and the lipid peroxidation by-products were assayed.

Results

Compared to the normal control and the control liquid diet groups, the activities of ALT, AST, and γ-GT, and the lipid peroxidation by-products increased significantly in the ethanol liquid diet group. The pathological changes of cellular swelling and fatty degeneration in the ethanol liquid diet group were severe.

Conclusion

Alcoholic hepatic injury model can be successfully duplicated by Lieber-DeCarli prescription.

Key words: Alcohol, Hepatic injury, Ultrastructure, Rats

CLC Number:

R575

Zhi-hong Fang, Jian-wei Cui, Jian-mei Cao, Jing-hua Peng, Qin Feng, Yi-yang Hu. Preparation of Lieber-DeCarli liquid diet induced alcoholic hepatic injury model in rats. Journal of Chinese Integrative Medicine, 2006, 4(6): 595-600.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20060611

http://www.jcimjournal.com/EN/Y2006/V4/I6/595

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Figures/Tables 4

Table 1

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Table 2

Table 3

References 7

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[2]	Lieber CS , DeCarli LM.The feeding of alcohol in liquid diets: two decades of applications and 1982 update[J]. Alcohol Clin Exp Res, 1982,6(4):523-531
[3]	中华医学会肝脏病学分会脂肪肝和酒精性肝病学组. 酒精性肝病诊断标准[J]. 中华肝脏病杂志, 2003,11(2):72
[4]	Lieber CS, Jones DP , DeCarli LM.Effects of prolonged ethanol intake: production of fatty liver despite adequate diets[J]. J Clin Invest, 1965,44(6):1009-1021
[5]	李舒丹, 厉有名, 虞朝辉 . 大鼠慢性酒精性肝损伤模型的建立[J]. 浙江医学, 2002,24(9):524-525, 530 doi: 10.3969/j.issn.1006-2785.2002.09.005
[6]	范建高, 曾民德 .脂肪肝 ( 第1版)[M]. 上海: 上海医科大学出版社, 2000,( 第1版):91-92
[7]	Lieber CS . Alcohol and liver: 1994 update[J]. Gastroenterology, 1994,106(4):1085-1105 doi: 10.1177/146642409411400212 pmid: 8143977

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Group	n	Body weight at the beginning of modeling (g)	Body weight at the end of modeling (g)	Average total intake (ml)	Ratio of liver weight and body weight (g/kg)
Control liquid diet	9	224±8	397±15	3 781±30	31.27±4.78
Ethanol liquid diet	9	227±9	375±15*	3 842±19	36.95±2.26*

Group	n	ALT (U/L)	AST (U/L)	γ-GT (U/g prot)
Normal control group	5	28.0±11.9	66.1±12.1	106±81
Control liquid diet group	9	17.4±6.6	71.0±13.8	100±50
Ethanol liquid diet group	9	75.1±44.8**	108.2±27.0**	493±132**

Group	n	MDA	NOS	iNOS	SOD	GSH	Anti-ROS unit
Group	n	(nmol/mg prot)	(U/mg prot)	(U/mg prot)	(U/mg prot)	(mg/g prot)	(U/mg prot)
Normal control group	5	6.93±1.09	5.5±1.4	0.5±0.3	118±15	45.9±3.9	235.8±53.6
Control liquid diet group	9	5.92±0.97	7.7±1.5	1.3±0.7	133±11	49.1±4.6	236.5±81.8
Ethanol liquid diet group	9	9.28±2.41**	24.8±5.8**	16.5±5.5**	99±12**	51.3±3.1	78.1±49.0**

Preparation of Lieber-DeCarli liquid diet induced alcoholic hepatic injury model in rats

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