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Journal of Chinese Integrative Medicine ›› 2006, Vol. 4 ›› Issue (6): 595-600.doi: 10.3736/jcim20060611

• Original Experimental Research • Previous Articles     Next Articles

Preparation of Lieber-DeCarli liquid diet induced alcoholic hepatic injury model in rats

Zhi-hong Fang,Jian-wei Cui,Jian-mei Cao,Jing-hua Peng,Qin Feng,Yi-yang Hu   

  1. Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
  • Online:2006-11-30 Published:2006-11-15


Duplicating the classical alcoholic hepatic injury model, to provide an ideal animal model for research on prevention and treatment of hepatic injury.


According to the prescription of Lieber-DeCarli, the same calorie fluid animal feed, which contained ethanol or non-ethanol, was prepared. Twenty-three rats were divided into normal control group (n=5), control liquid diet group (n=9), ethanol liquid diet group (n=9). Rats in the normal control group were fed normal diet, and rats in the control liquid diet group and ethanol liquid diet group were fed the corresponding diet for eight weeks. The pathologic changes of hepatic tissue were observed. The activities of the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the liver tissue γ-glutamyltransferase (γ-GT), the content of triglyceride (TG), and the lipid peroxidation by-products were assayed.


Compared to the normal control and the control liquid diet groups, the activities of ALT, AST, and γ-GT, and the lipid peroxidation by-products increased significantly in the ethanol liquid diet group. The pathological changes of cellular swelling and fatty degeneration in the ethanol liquid diet group were severe.


Alcoholic hepatic injury model can be successfully duplicated by Lieber-DeCarli prescription.

Key words: Alcohol, Hepatic injury, Ultrastructure, Rats

CLC Number: 

  • R575

Table 1

Liquid diet intake, body weight and the ratio of liver weight and body weight of rats in control liquid diet group and ethanol liquid diet group(ヌ±S)"

Group n Body weight at the beginning of modeling (g) Body weight at the end of modeling (g) Average total intake (ml) Ratio of liver weight and body weight (g/kg)
Control liquid diet 9 224±8 397±15 3 781±30 31.27±4.78
Ethanol liquid diet 9 227±9 375±15* 3 842±19 36.95±2.26*

Figure 1

Morphology of liver tissue and ultrastructures of hepatocytes"

Table 2

Activities of γ-GT in liver tissue, and ALT and AST in serum (ヌ±S)"

Group n ALT (U/L) AST (U/L) γ-GT (U/g prot)
Normal control group 5 28.0±11.9 66.1±12.1 106±81
Control liquid diet group 9 17.4±6.6 71.0±13.8 100±50
Ethanol liquid diet group 9 75.1±44.8** 108.2±27.0** 493±132**

Table 3

Contents of MDA and GSH, activities of NOS, iNOS and SOD, and anti-ROS unit in liver tissue of rats(?x±s)"

Group n MDA NOS iNOS SOD GSH Anti-ROS unit
(nmol/mg prot) (U/mg prot) (U/mg prot) (U/mg prot) (mg/g prot) (U/mg prot)
Normal control group 5 6.93±1.09 5.5±1.4 0.5±0.3 118±15 45.9±3.9 235.8±53.6
Control liquid diet group 9 5.92±0.97 7.7±1.5 1.3±0.7 133±11 49.1±4.6 236.5±81.8
Ethanol liquid diet group 9 9.28±2.41** 24.8±5.8** 16.5±5.5** 99±12** 51.3±3.1 78.1±49.0**
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