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Journal of Chinese Integrative Medicine ›› 2007, Vol. 5 ›› Issue (1): 65-68.doi: 10.3736/jcim20070113

• Original Experimental Research • Previous Articles     Next Articles

Effects of inactivated rabbit serum containing compound realgar and natural indigo tablet on cell line NB4

Nan-nan Chen1, Shi-lin Huang2 , Yang Xiang2, De-jie Zhang2, Ai-xia Guo2, Ai-ping Chen2   

  1. 1. Graduate School of General Hospital of People’s Liberation Army,Beijing 100853, China;
    2. Department of Traditional Chinese Medicine and Hematology, Dalian 210th Hospital of People’s Liberation Army, Dalian, Liaoning Province 116021, China;
  • Online:2007-01-31 Published:2007-01-21

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Objective: To explore the effects of inactivated rabbit serum containing compound realgar and natural indigo tablet (CRNIT) on cell line NB4. 

Methods: The experimental rabbits were taken as the provider of the animal serum, and the serum was inactivated before the experiment. The serum was divided into two groups based on whether the rabbits were given CRNIT. The concentration of arsenic in the rabbit's serum was detected by AFS-230a double path atom fluorescence photometer. The inhibition rates and apoptosis rates were regarded as the observational indexes.

Results: The concentration of arsenic in the inactivated rabbit serum containing and not containing the drug were (0.010 0±0.001 0) mg/L and (0.110 0±0.006 4) mg/L respectively, and the difference had statistical significance (P＜0.01). The two groups of serum all had inhibitory effect on the growth of NB4 cells depending on the drug concentration and effect time. And there were significant differences among the groups. The two groups of serum all induced the apoptosis of NB4 with positive relations with the concentration and effect time. And there were significant differences among the groups.

Conclusion: The rabbit serum containing CRNIT can obviously restrain the growth of NB4 cells and the inhibitory effect depends on the concentration and effect time. And the rabbit serum containing CRNIT can also induce the apoptosis of NB4 cell line and the apoptosis rates depend on the concentration and effect time.

Key words: rabbits, apoptosis, serum, leukemia, Chinese herbal drugs

CLC Number: 

  • R693

Table 1

Inhibition rates of NB4 cells after 24- and 48-hour treatment of two groups of rabbit serum ($\bar{x}±S$,%)"

Apoptosis rates of NB4 cells after 24- and 48-hour treatment of two groups of rabbit serum ($\bar{x}±S$,%)"

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