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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (9): 831-835.doi: 10.3736/jcim20090907

• Original Experimental Research • Previous Articles     Next Articles

Effects of extracts and active components of Rhizoma Coptidis on contraction of circular smooth muscle isolated from guinea pig gastric antrum

Jian-ye Yuana,b, De-gao Zhangb,YU Xiaoa,WU Da-zhenga   

  1. a Institute of Chinese Materia Medica, Shanghai Key Laboratory of Complex Prescription, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    b Institute of Digestive Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2009-05-31 Accepted:2009-07-15 Online:2009-09-20 Published:2009-09-15
  • Contact: Da-zheng Wu E-mail:wdazheng@hotmail.com

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Objective

To identify the influence of extracts and active components of Rhizoma Coptidis on gastric smooth muscle contractility of guinea pigs, and to explore the potential pharmacological mechanism of Rhizoma Coptidis in "invigorating the stomach" and "impairing the stomach".
Methods

Observing the effects of the water extract and the alkaloids from Rhizoma Coptidis (at doses ranging from 0.3 to 1 000 μg/L) and other active components such as berberine, palmatine and jatrorrhizine (at doses ranging from 0.3 to 1 000 μmol/L) on the spontaneous and electrical field stimulation (EFS)-induced contractions of antral circular smooth muscle strips from guinea pig stomach via a force transducer in vitro.
Results

The water extract or the alkaloids from Rhizoma Coptidis could improve the spontaneous contraction at the low doses, but inhibit the spontaneous contraction at the high doses. Berberine, palmatine and jatrorrhizine also showed the similar effects. Moreover, the water extract and the alkaloids of Rhizoma Coptidis, as well as berberine, palmatine and jatrorrhizine could increase the EFS-induced contraction. Among the three monomers, jatrorrhizine exhibited the most potent effect on EFS-induced contraction.
Conclusion

The effects of Rhizoma Coptidis in "invigorating the stomach" or "impairing the stomach" may be related to its effect on gastric smooth muscle contractility. Berberine, palmatine and jatrorrhizine are all effective components of Rhizoma Coptidis affecting the contraction of gastric smooth muscle, among which jatrorrhizine is the most potent agent in promoting the contraction while berberine is the most potent one for inhibiting the contraction.

Key words: Rhizoma Coptidis, Invigorating the stomach, Impairing the stomach, Smooth muscle, Muscle contraction, Guinea pigs

Figure 1

Effects of water extracts and alkaloids of Rhizoma Coptidis on amplitude of spontaneous contraction of the circular smooth muscle strips of guinea pig gastric antrum The concentration-responsa curve of the water extract (from 0.3 to 1 000 μg/mL) and the alkaloids (from 0.3 to 300 μg/mL) of Rhizoma Coptidis on amplitude of the spontaneous contraction. Data points represent $\bar{x}$±s, n=6."

Figure 2

Effects of berberine, palmatine and jatrorrhizine on amplitude of spontaneous contraction of the circular smooth muscle strips in guinea pig gastric antrum The concentration-response curve of berberine, palmatine and jatrorrhizine (from 0.3 to 1 000 μmol/L) on amplitude of the spontaneous contraction. Data points represent $\bar{x}$±s, n=4 or 5."

Figure 3

Effects of water extracts and alkaloids of Rhizoma Coptidis on EFS-induced contraction of the circular smooth muscle strips in guinea pig gastric antrum A: Effects of the water extracts of Rhizoma Coptidis (3, 30 and 300 μg/mL) on EFS-induced contraction. B: Effects of the alkaloids of Rhizoma Coptidis (3, 30 and 300 μg/mL) on EFS-induced contraction. Electric stimulation parameters: 1 Hz, 50 V and 1 ms duration for 2 minutes. **P<0.01, vs control group. Data were represented as $\bar{x}$±s, n=6."

Figure 4

Effects of berberine, palmatine and jatrorrhizine on EFS-induced contraction of the circular smooth muscle strips in guinea pig gastric antrum A: Effects of berberine (3, 30 and 300 μmol/L) on EFS-induced contraction; B: Effects of palmatine (3, 30 and 300 μmol/L) on EFS-induced contraction; C: Effects of jatrorrhizine (3, 30 and 300 μmol/L) on EFS-induced contraction. Electric stimulation parameters: 1 Hz, 50 V and 1 ms duration for 2 minutes. *P<0.05, **P<0.01, vs control. Data were represented as $\bar{x}$s, n=6."

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