SUBSCRIBE
Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (9): 831-835.doi: 10.3736/jcim20090907

• Original Experimental Research • Previous Articles     Next Articles

Effects of extracts and active components of Rhizoma Coptidis on contraction of circular smooth muscle isolated from guinea pig gastric antrum

Jian-ye Yuana,b, De-gao Zhangb,YU Xiaoa,WU Da-zhenga   

  1. a Institute of Chinese Materia Medica, Shanghai Key Laboratory of Complex Prescription, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    b Institute of Digestive Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2009-05-31 Accepted:2009-07-15 Online:2009-09-20 Published:2009-09-15
  • Contact: Da-zheng Wu E-mail:wdazheng@hotmail.com

RichHTML

0

PDF (PC)

55

Like

Objective

To identify the influence of extracts and active components of Rhizoma Coptidis on gastric smooth muscle contractility of guinea pigs, and to explore the potential pharmacological mechanism of Rhizoma Coptidis in "invigorating the stomach" and "impairing the stomach".
Methods

Observing the effects of the water extract and the alkaloids from Rhizoma Coptidis (at doses ranging from 0.3 to 1 000 μg/L) and other active components such as berberine, palmatine and jatrorrhizine (at doses ranging from 0.3 to 1 000 μmol/L) on the spontaneous and electrical field stimulation (EFS)-induced contractions of antral circular smooth muscle strips from guinea pig stomach via a force transducer in vitro.
Results

The water extract or the alkaloids from Rhizoma Coptidis could improve the spontaneous contraction at the low doses, but inhibit the spontaneous contraction at the high doses. Berberine, palmatine and jatrorrhizine also showed the similar effects. Moreover, the water extract and the alkaloids of Rhizoma Coptidis, as well as berberine, palmatine and jatrorrhizine could increase the EFS-induced contraction. Among the three monomers, jatrorrhizine exhibited the most potent effect on EFS-induced contraction.
Conclusion

The effects of Rhizoma Coptidis in "invigorating the stomach" or "impairing the stomach" may be related to its effect on gastric smooth muscle contractility. Berberine, palmatine and jatrorrhizine are all effective components of Rhizoma Coptidis affecting the contraction of gastric smooth muscle, among which jatrorrhizine is the most potent agent in promoting the contraction while berberine is the most potent one for inhibiting the contraction.

Key words: Rhizoma Coptidis, Invigorating the stomach, Impairing the stomach, Smooth muscle, Muscle contraction, Guinea pigs

Figure 1

Effects of water extracts and alkaloids of Rhizoma Coptidis on amplitude of spontaneous contraction of the circular smooth muscle strips of guinea pig gastric antrum The concentration-responsa curve of the water extract (from 0.3 to 1 000 μg/mL) and the alkaloids (from 0.3 to 300 μg/mL) of Rhizoma Coptidis on amplitude of the spontaneous contraction. Data points represent $\bar{x}$±s, n=6."

Figure 2

Effects of berberine, palmatine and jatrorrhizine on amplitude of spontaneous contraction of the circular smooth muscle strips in guinea pig gastric antrum The concentration-response curve of berberine, palmatine and jatrorrhizine (from 0.3 to 1 000 μmol/L) on amplitude of the spontaneous contraction. Data points represent $\bar{x}$±s, n=4 or 5."

Figure 3

Effects of water extracts and alkaloids of Rhizoma Coptidis on EFS-induced contraction of the circular smooth muscle strips in guinea pig gastric antrum A: Effects of the water extracts of Rhizoma Coptidis (3, 30 and 300 μg/mL) on EFS-induced contraction. B: Effects of the alkaloids of Rhizoma Coptidis (3, 30 and 300 μg/mL) on EFS-induced contraction. Electric stimulation parameters: 1 Hz, 50 V and 1 ms duration for 2 minutes. **P<0.01, vs control group. Data were represented as $\bar{x}$±s, n=6."

Figure 4

Effects of berberine, palmatine and jatrorrhizine on EFS-induced contraction of the circular smooth muscle strips in guinea pig gastric antrum A: Effects of berberine (3, 30 and 300 μmol/L) on EFS-induced contraction; B: Effects of palmatine (3, 30 and 300 μmol/L) on EFS-induced contraction; C: Effects of jatrorrhizine (3, 30 and 300 μmol/L) on EFS-induced contraction. Electric stimulation parameters: 1 Hz, 50 V and 1 ms duration for 2 minutes. *P<0.05, **P<0.01, vs control. Data were represented as $\bar{x}$s, n=6."

[1] Chen FX, Gao XS . Statistics of formulas containing Rhizoma Coptidis[J]. Zhong Cheng Yao, 1997,19(8):40-41
陈馥馨, 高晓山 . 含黄连方剂及黄连配伍药的文献统计[J]. 中成药, 1997,19(8):40-41
[2] Liu GW. Chinese materia medica[M]. Beijing: Huaxia Publishing Co., Ltd, 2000: 81
刘公望 . 中药学[M]. 北京: 华夏出版社, 2000: 81
[3] Chen BX. The newly-revised essentials of materia medica[M]. Shanghai: Traditional Chinese Medicine Publishing House, 1955: 21
陈邦贤 . 新本草备要[M]. 上海: 中医书局, 1955: 21
[4] Zhang ZC. Scientific illustration for essentials of materia medica[M]. Shanghai: Shanghai Medical Publishing House, 1956: 187
张赞臣 . 科学注解本草概要[M]. 上海: 上海卫生出版社, 1956: 187
[5] Lei ZQ. Chinese materia medica(6th ed)[M]. Shanghai: Shanghai Scientific & Technical Publishers, 2000,( 6th ed):62
雷载权 . 中药学(x第6版)[M]. 上海: 上海科学技术出版社, 2000,( 第6版):62
[6] Li XX . Approach to the theory of bitter-cold medicine induced stomach injury[J]. Zhongguo Zhong Yao Za Zhi, 2001,26(11):792-794
doi: 10.3321/j.issn:1001-5302.2001.11.028
李希新 . 对苦寒药伤胃的理论探讨[J]. 中国中药杂志, 2001,26(11):792-794
doi: 10.3321/j.issn:1001-5302.2001.11.028
[7] Chu SL . The benefit of eating bitter[J]. Shandong Shi Pin Ke Ji, 2004,6(7):23
褚双林 . 吃"苦"的甜头[J]. 山东食品科技, 2004,6(7):23
[8] Burks TF, Galligan JJ, Porreca F, Barber WD . Regulation of gastric emptying[J]. Fed Proc, 1985,44(14):2897-2901
[9] Ogishima M, Kaibara M, Ueki S, Kurimoto T, Taniyama K . Z-338 facilitates acetylcholine release from enteric neurons due to blockade of muscarinic autoreceptors in guinea pig stomach[J]. J Pharmacol Exp Ther, 2000,294(1):33-37
[10] Ingkaninan K, Phengpa P, Yuenyongsawad S, Khorana N . Acetylcholinesterase inhibitors from Stephania venosa tuber[J]. J Pharm Pharmacol, 2006,58(5):695-700
doi: 10.1211/jpp.58.5.0015 pmid: 16640839
[1] Bang-jiang Fang, Shuang Zhou, Xin-jun Pei, Jin-yang Huang, Bao-jin Chen, Yun Geng , Li-kun Yang. Effects of Yanggan Lidan Granule on insulin resistance in guinea pigs with induced cholesterol gallstones. Journal of Chinese Integrative Medicine, 2009, 7(12): 1159-1163.
[2] Hua Li, Gang Chen, Chang-qing Deng. Effects of plasma containing Buyang Huanwu Decoction and its effective components and total Panax notoginseng saponins on vascular smooth muscle cell proliferation induced by platelet-derived growth factor. Journal of Chinese Integrative Medicine, 2009, 7(11): 1078-1085.
[3] Jian-ye Yuan, Jian-qun Xie, Da-zheng Wu, Yu Zheng, Xiang-xue Pan, Xiao-yan Fei, Hai-zhen Xu. Tongxie Yaofang inhibits the contraction of colonic smooth muscle isolated from rats through a mechanism related to calcium mobilization. Journal of Chinese Integrative Medicine, 2009, 7(10): 958-962.
[4] Zheng-gao Xie, Xing-wei Wu, Chao-rong Zhuang, Fang Chen, Zheng Wang, Ya-kun Wang, Xin Hua. Protective effects of Ginkgo biloba extract on morphology and function of retinal ganglion cells after optic nerve transection in guinea pigs. Journal of Chinese Integrative Medicine, 2009, 7(10): 940-945.
[5] Lu Wu, Wei Zhang, Hua Li, Guo-min Zhang, Bei-yang Chen, Ying-hong Tang, Chang-qing Deng. Effects of Buyang Huanwu Decoction and its alkaloids and glycosides on aortic intimal hyperplasia and expression of proliferating cell nuclear antigen in rats with aortic intimal injuries. Journal of Chinese Integrative Medicine, 2008, 6(8): 836-842.
[6] Hong-di Huan, Jing-hong Zhang, Shi-rong Zheng, Wei-wei Meng, Jun-li Zhang. Dilingdan Decoction prevents renal interstitial fibrosis in a rat model of unilateral ureteral obstruction. Journal of Chinese Integrative Medicine, 2008, 6(5): 493-497.
[7] Jiong Li, Jing-zhe Zhang, Chang-peng Zou, Bang-jiang Fang, Xiao-qiang Liang. Shengqing Capsule down-regulates estrogen and progesterone receptors in epithelial tissue of gallbladder in guinea pigs with gallstone. Journal of Chinese Integrative Medicine, 2008, 6(10): 1040-1044.
[8] Ping Shen, Bang-jiang Fang, Pei-ting Zhu, Jing-zhe Zhang, Xin-jun Pei. Effect of traditional Chinese herbs for nourishing the liver on intracellular free calcium level in gallbladder cells of guinea pigs with gallstones. Journal of Chinese Integrative Medicine, 2007, 5(2): 179-182.
[9] Hong-gang Gu, Jing-zhe Zhang, Xue-lin Zhang, Jian-wen Liu, Ting Li, Pei-ting Zhu. Effects of Shengqing Capsules on expression of nuclear factor-κB protein in liver cells of guinea pigs with pigment gallstone. Journal of Chinese Integrative Medicine, 2006, 4(5): 518-521.
[10] Bo Ji​, Ping Geng​, Jian-gang Liu​, Da-zhuo Shi​, Yong-yan Wang​. Effects of active components extracted from Qixue Bingzhi Recipe on proliferation of vascular smooth muscle cells and expressions of platelet-derived growth factor and its receptor genes. Journal of Chinese Integrative Medicine, 2006, 4(1): 30-34.
[11] Jin-kun Wen, Mei Han. Supplementing Qi and activating blood circulation in gene regulation of vascular smooth muscle cells. Journal of Chinese Integrative Medicine, 2003, 1(1): 12-14.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228
Journal of Integrative Medicine, ISSN: 2095-4964 CN 31-2083/R
Copyright © Journal of Integrative Medicine
Powered by Beijing Magtech Co. Ltd
沪公网安备31010602003416号      沪ICP备18017633号-3