Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (9): 831-835.doi: 10.3736/jcim20090907

• Original Experimental Research • Previous Articles     Next Articles

Effects of extracts and active components of Rhizoma Coptidis on contraction of circular smooth muscle isolated from guinea pig gastric antrum

Jian-ye Yuana,b, De-gao Zhangb,YU Xiaoa,WU Da-zhenga   

  1. a Institute of Chinese Materia Medica, Shanghai Key Laboratory of Complex Prescription, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    b Institute of Digestive Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2009-05-31 Accepted:2009-07-15 Online:2009-09-20 Published:2009-09-15
  • Contact: Da-zheng Wu E-mail:wdazheng@hotmail.com

Objective

To identify the influence of extracts and active components of Rhizoma Coptidis on gastric smooth muscle contractility of guinea pigs, and to explore the potential pharmacological mechanism of Rhizoma Coptidis in "invigorating the stomach" and "impairing the stomach".
Methods

Observing the effects of the water extract and the alkaloids from Rhizoma Coptidis (at doses ranging from 0.3 to 1 000 μg/L) and other active components such as berberine, palmatine and jatrorrhizine (at doses ranging from 0.3 to 1 000 μmol/L) on the spontaneous and electrical field stimulation (EFS)-induced contractions of antral circular smooth muscle strips from guinea pig stomach via a force transducer in vitro.
Results

The water extract or the alkaloids from Rhizoma Coptidis could improve the spontaneous contraction at the low doses, but inhibit the spontaneous contraction at the high doses. Berberine, palmatine and jatrorrhizine also showed the similar effects. Moreover, the water extract and the alkaloids of Rhizoma Coptidis, as well as berberine, palmatine and jatrorrhizine could increase the EFS-induced contraction. Among the three monomers, jatrorrhizine exhibited the most potent effect on EFS-induced contraction.
Conclusion

The effects of Rhizoma Coptidis in "invigorating the stomach" or "impairing the stomach" may be related to its effect on gastric smooth muscle contractility. Berberine, palmatine and jatrorrhizine are all effective components of Rhizoma Coptidis affecting the contraction of gastric smooth muscle, among which jatrorrhizine is the most potent agent in promoting the contraction while berberine is the most potent one for inhibiting the contraction.

Key words: Rhizoma Coptidis, Invigorating the stomach, Impairing the stomach, Smooth muscle, Muscle contraction, Guinea pigs

Figure 1

Effects of water extracts and alkaloids of Rhizoma Coptidis on amplitude of spontaneous contraction of the circular smooth muscle strips of guinea pig gastric antrum The concentration-responsa curve of the water extract (from 0.3 to 1 000 μg/mL) and the alkaloids (from 0.3 to 300 μg/mL) of Rhizoma Coptidis on amplitude of the spontaneous contraction. Data points represent $\bar{x}$±s, n=6."

Figure 2

Effects of berberine, palmatine and jatrorrhizine on amplitude of spontaneous contraction of the circular smooth muscle strips in guinea pig gastric antrum The concentration-response curve of berberine, palmatine and jatrorrhizine (from 0.3 to 1 000 μmol/L) on amplitude of the spontaneous contraction. Data points represent $\bar{x}$±s, n=4 or 5."

Figure 3

Effects of water extracts and alkaloids of Rhizoma Coptidis on EFS-induced contraction of the circular smooth muscle strips in guinea pig gastric antrum A: Effects of the water extracts of Rhizoma Coptidis (3, 30 and 300 μg/mL) on EFS-induced contraction. B: Effects of the alkaloids of Rhizoma Coptidis (3, 30 and 300 μg/mL) on EFS-induced contraction. Electric stimulation parameters: 1 Hz, 50 V and 1 ms duration for 2 minutes. **P<0.01, vs control group. Data were represented as $\bar{x}$±s, n=6."

Figure 4

Effects of berberine, palmatine and jatrorrhizine on EFS-induced contraction of the circular smooth muscle strips in guinea pig gastric antrum A: Effects of berberine (3, 30 and 300 μmol/L) on EFS-induced contraction; B: Effects of palmatine (3, 30 and 300 μmol/L) on EFS-induced contraction; C: Effects of jatrorrhizine (3, 30 and 300 μmol/L) on EFS-induced contraction. Electric stimulation parameters: 1 Hz, 50 V and 1 ms duration for 2 minutes. *P<0.05, **P<0.01, vs control. Data were represented as $\bar{x}$s, n=6."

[1] Chen FX, Gao XS . Statistics of formulas containing Rhizoma Coptidis[J]. Zhong Cheng Yao, 1997,19(8):40-41
陈馥馨, 高晓山 . 含黄连方剂及黄连配伍药的文献统计[J]. 中成药, 1997,19(8):40-41
[2] Liu GW. Chinese materia medica[M]. Beijing: Huaxia Publishing Co., Ltd, 2000: 81
刘公望 . 中药学[M]. 北京: 华夏出版社, 2000: 81
[3] Chen BX. The newly-revised essentials of materia medica[M]. Shanghai: Traditional Chinese Medicine Publishing House, 1955: 21
陈邦贤 . 新本草备要[M]. 上海: 中医书局, 1955: 21
[4] Zhang ZC. Scientific illustration for essentials of materia medica[M]. Shanghai: Shanghai Medical Publishing House, 1956: 187
张赞臣 . 科学注解本草概要[M]. 上海: 上海卫生出版社, 1956: 187
[5] Lei ZQ. Chinese materia medica(6th ed)[M]. Shanghai: Shanghai Scientific & Technical Publishers, 2000,( 6th ed):62
雷载权 . 中药学(x第6版)[M]. 上海: 上海科学技术出版社, 2000,( 第6版):62
[6] Li XX . Approach to the theory of bitter-cold medicine induced stomach injury[J]. Zhongguo Zhong Yao Za Zhi, 2001,26(11):792-794
doi: 10.3321/j.issn:1001-5302.2001.11.028
李希新 . 对苦寒药伤胃的理论探讨[J]. 中国中药杂志, 2001,26(11):792-794
doi: 10.3321/j.issn:1001-5302.2001.11.028
[7] Chu SL . The benefit of eating bitter[J]. Shandong Shi Pin Ke Ji, 2004,6(7):23
褚双林 . 吃"苦"的甜头[J]. 山东食品科技, 2004,6(7):23
[8] Burks TF, Galligan JJ, Porreca F, Barber WD . Regulation of gastric emptying[J]. Fed Proc, 1985,44(14):2897-2901
[9] Ogishima M, Kaibara M, Ueki S, Kurimoto T, Taniyama K . Z-338 facilitates acetylcholine release from enteric neurons due to blockade of muscarinic autoreceptors in guinea pig stomach[J]. J Pharmacol Exp Ther, 2000,294(1):33-37
[10] Ingkaninan K, Phengpa P, Yuenyongsawad S, Khorana N . Acetylcholinesterase inhibitors from Stephania venosa tuber[J]. J Pharm Pharmacol, 2006,58(5):695-700
doi: 10.1211/jpp.58.5.0015 pmid: 16640839
[1] Bang-jiang Fang, Shuang Zhou, Xin-jun Pei, Jin-yang Huang, Bao-jin Chen, Yun Geng , Li-kun Yang. Effects of Yanggan Lidan Granule on insulin resistance in guinea pigs with induced cholesterol gallstones. Journal of Chinese Integrative Medicine, 2009, 7(12): 1159-1163.
[2] Hua Li, Gang Chen, Chang-qing Deng. Effects of plasma containing Buyang Huanwu Decoction and its effective components and total Panax notoginseng saponins on vascular smooth muscle cell proliferation induced by platelet-derived growth factor. Journal of Chinese Integrative Medicine, 2009, 7(11): 1078-1085.
[3] Zheng-gao Xie, Xing-wei Wu, Chao-rong Zhuang, Fang Chen, Zheng Wang, Ya-kun Wang, Xin Hua. Protective effects of Ginkgo biloba extract on morphology and function of retinal ganglion cells after optic nerve transection in guinea pigs. Journal of Chinese Integrative Medicine, 2009, 7(10): 940-945.
[4] Jian-ye Yuan, Jian-qun Xie, Da-zheng Wu, Yu Zheng, Xiang-xue Pan, Xiao-yan Fei, Hai-zhen Xu. Tongxie Yaofang inhibits the contraction of colonic smooth muscle isolated from rats through a mechanism related to calcium mobilization. Journal of Chinese Integrative Medicine, 2009, 7(10): 958-962.
[5] Lu Wu, Wei Zhang, Hua Li, Guo-min Zhang, Bei-yang Chen, Ying-hong Tang, Chang-qing Deng. Effects of Buyang Huanwu Decoction and its alkaloids and glycosides on aortic intimal hyperplasia and expression of proliferating cell nuclear antigen in rats with aortic intimal injuries. Journal of Chinese Integrative Medicine, 2008, 6(8): 836-842.
[6] Hong-di Huan, Jing-hong Zhang, Shi-rong Zheng, Wei-wei Meng, Jun-li Zhang. Dilingdan Decoction prevents renal interstitial fibrosis in a rat model of unilateral ureteral obstruction. Journal of Chinese Integrative Medicine, 2008, 6(5): 493-497.
[7] Jiong Li, Jing-zhe Zhang, Chang-peng Zou, Bang-jiang Fang, Xiao-qiang Liang. Shengqing Capsule down-regulates estrogen and progesterone receptors in epithelial tissue of gallbladder in guinea pigs with gallstone. Journal of Chinese Integrative Medicine, 2008, 6(10): 1040-1044.
[8] Ping Shen, Bang-jiang Fang, Pei-ting Zhu, Jing-zhe Zhang, Xin-jun Pei. Effect of traditional Chinese herbs for nourishing the liver on intracellular free calcium level in gallbladder cells of guinea pigs with gallstones. Journal of Chinese Integrative Medicine, 2007, 5(2): 179-182.
[9] Hong-gang Gu, Jing-zhe Zhang, Xue-lin Zhang, Jian-wen Liu, Ting Li, Pei-ting Zhu. Effects of Shengqing Capsules on expression of nuclear factor-κB protein in liver cells of guinea pigs with pigment gallstone. Journal of Chinese Integrative Medicine, 2006, 4(5): 518-521.
[10] Bo Ji​, Ping Geng​, Jian-gang Liu​, Da-zhuo Shi​, Yong-yan Wang​. Effects of active components extracted from Qixue Bingzhi Recipe on proliferation of vascular smooth muscle cells and expressions of platelet-derived growth factor and its receptor genes. Journal of Chinese Integrative Medicine, 2006, 4(1): 30-34.
[11] Jin-kun Wen, Mei Han. Supplementing Qi and activating blood circulation in gene regulation of vascular smooth muscle cells. Journal of Chinese Integrative Medicine, 2003, 1(1): 12-14.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. An explanation on "guiding principles of clinical research on mild cognitive impairment (protocol)". Journal of Chinese Integrative Medicine, 2008, 6(1): 15-21
[3] Jin-rong Fu. Establishment of multivariate diagnosis and treatment system of modern gynecology of traditional Chinese medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 22-24
[4] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[5] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[6] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[7] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[8] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[9] Wei Zhang, Xiang-feng Lu, Xiao-mei Zhang, Jian-jun Wu, Liang-duo Jiang. A rat model of pulmonary fibrosis induced by infusing bleomycin quickly through tracheal intubation. Journal of Chinese Integrative Medicine, 2008, 6(1): 60-67
[10] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76