Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (4): 354-359.doi: 10.3736/jcim20090410

• Original Experimental Research • Previous Articles     Next Articles

Effects of San'ao decoction and its analogous prescriptions on airway inflammation in mice with respiratory syncytial virus- and ovalbumin-induced asthma

Ying Zhanga,Huang-jin Tonga,Jing-hua Yub,Peng-cheng Gua,Xin-sheng Fanc,Hui-qin Xua   

  1. a Department of Pharmacology, Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
    b Institute of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
    c Jiangsu Key Laboratory for Traditional Chinese Medicine Formula Research, Nanjing University of Chinese Medicine, Nanjing 210046, Jiangsu Province, China
  • Received:2008-11-28 Accepted:2009-02-04 Online:2009-04-20 Published:2009-04-15

Objective

To evaluate the effects of San'ao decoction (SAD) and its analogous prescriptions (APs), compounds of traditional Chinese herbal medicine for asthma, on airway inflammation in mice with respiratory syncytial virus (RSV)- and ovalbumin (OVA)-induced asthma.
Methods

A total of 110 mice were randomly divided into control group, untreated group, dexamethasone (DM) group, small-dose SAD (SAD-S) group, large-dose SAD (SAD-L) group, APⅠ-S group, APⅠ-L group, APⅡ-S group, APⅡ-L group, APⅢ-S group, and APⅢ-L group. The asthma model was reproduced by sensitization with multipoint intraperitoneal injection of OVA, followed by repeated inhalation of OVA combined with intranasal instillation of RSV. Cells in bronchoalveolar lavage fluid (BALF) were counted and classified. The supernatant of the BALF was used for detecting the contents of interleukin-4 (IL-4), interleukin-5 (IL-5) and interferon-gamma (IFN-γ) by enzyme-linked immunosorbent assay. Pathological changes in lung tissue were observed by hematoxilin and eosin staining and the scores of pathological changes were also calculated to determine the degree of inflammation.
Results

Compared with the control group, the amounts of lymphocytes, eosinophils, neutrophils in BALF in the untreated group were increased significantly (P<0.01); the changes of lung histopathology in the untreated group were much more serious, and the content of IFN-γ was sharply decreased, while the contents of IL-4 and IL-5 were significantly increased (P<0.05). The counts of eosinophils in BALF of the treated groups all decreased obviously (P<0.01) as compared with the untreated group. The count of the neutrophils in BALF of the APⅡ-L group was obviously lower than that in the untreated group (P<0.01). Most of Chinese herbal formulas and DM could increase the level of IFN-γ, and decrease the level of IL-4. All concentrations of the APs and SAD could decrease the level of IL-5 as compared with the untreated group, especially of the APⅡ-L and APⅠ-L (P<0.05, P<0.01).
Conslusion

SAD and its APs had some therapeutic effects on RSV-induced asthma in mice. Among the formulas, APⅡ has a better therapeutic efficacy in treatment of asthma by decreasing the amount of neutrophils.

Key words: Respiratory syncytial virus, Asthma, San'ao decoction, Neutrophils, Mice

Table 1

Differential counts about inflammatory cells in BALF in each group (x±s, ×105/mL)"

Group Dose (g/kg) n Cell count
Macrophages Lymphocytes Eosinophils Neutrophils
Control 10 10.14±2.30 2.23±1.00 0 0
Untreated 10 35.17±19.49 31.45±18.15** 3.83±1.24** 13.60±4.13**
DM 0.002 4 10 16.21±4.50 3.71±1.65 0.66±0.66△△ 9.45±8.31
SAD-S 4.4 10 11.33±2.92 5.06±0.65**▲▲ 0.34±0.18△△ 6.45±4.91
SAD-L 8.8 10 13.12±4.60 14.63±6.25* 0.59±0.47△△ 6.70±3.85
APⅠ-S 7.4 10 6.55±2.07 4.92±2.19 0.34±0.29△△ 10.77±7.19
APⅠ-L 14.8 10 8.79±3.52 5.06±4.26 0.29±0.19△△ 9.67±4.68*
APⅡ-S 13.4 10 11.92±7.30 4.25±2.25 0.11±0.11△△ 6.75±5.22
APⅡ-L 26.8 10 13.95±7.17 0.73±0.64 0.42±0.50△△ 2.85±1.87△△
APⅢ-S 4.8 10 12.75±8.99 5.68±1.51**▲▲ 0.60±0.65△△ 14.03±11.78
APⅢ-L 9.6 10 18.55±6.89 7.23±3.60 0.58±0.51△△ 7.58±6.22

Table 2

Levels of IFN-γ, IL-4 and IL-5 in supernatant of BALF in each group (x±s, ng/L)"

Group Dose (g/kg) n IFN-γ IL-4 IL-5
Control 10 30.08±4.01 12.11±1.51 15.25±4.07
Untreated 10 18.77±4.01* 23.30±12.04* 40.15±10.02*
DM 0.002 4 10 44.43±19.39 11.71±1.59 18.29±3.97
SAD-S 4.4 10 42.68±16.89 30.12±23.51 21.93±9.78
SAD-L 8.8 10 62.09±35.64 19.97±13.81 19.80±9.71
APⅠ-S 7.4 10 17.70±3.19* 16.04±14.09 26.87±18.93
APⅠ-L 14.8 10 66.43±20.10 14.72±10.02 7.66±3.20△△
APⅡ-S 13.4 10 19.08±2.32* 12.27±2.60 19.02±1.64
APⅡ-L 26.8 10 29.02±11.60 11.57±0.62 9.05±2.32
APⅢ-S 4.8 10 59.20±24.30 21.39±14.48 21.67±16.70
APⅢ-L 9.6 10 65.72±38.40 19.77±15.62 14.69±8.98

Table 3

Scores of pathological change in each group (x±s)"

Group Dose (g/kg) n Pathological change score
Control 10 1.00±0.71
Untreated 10 5.00±1.27**
DM 0.002 4 10 2.00±0.79△△
SAD-S 4.4 10 4.20±1.30
SAD-L 8.8 10 4.80±2.08
APⅠ-S 7.4 10 2.80±0.76
APⅠ-L 14.8 10 3.10±1.88
APⅡ-S 13.4 10 2.20±1.61△△
APⅡ-L 26.8 10 2.50±0.71
APⅢ-S 4.8 10 2.70±1.20
APⅢ-L 9.6 10 4.20±2.51

Figure 1

Pathological changes of lung tissues observed by HE staining (Light microscopy, ×200) A: Control group; B: Untreated group; C: DM group; D: SAD-S group; E: SAD-L group; F: APⅠ-S group; G: APⅠ-L group; H: APⅡ-S group; I: APⅡ-L group; J: APⅢ-S group; K: APⅢ-L group."

[1] Tong HJ, Fan XS, Xu HQ, Wang L, Cui J . Reproduction of a type of viral asthma model in mice and its evaluation[J]. Xi’an Jiao Tong Da Xue Xue Bao Yi Xue Ban, 2008,29(3):349-352
童黄锦, 范欣生, 许惠琴, 王露, 崔姣 . 一种病毒性哮喘模型制作方法的建立及评价[J]. 西安交通大学学报(医学版), 2008,29(3):349-352
[2] Deng WW, Guo XJ. Allergic pulmonary disease[M]. Shanghai: Shanghai Scientific and Technical Publishers,2000: 58
邓伟吾, 郭雪君 . 肺部变应性疾病[M]. 上海: 上海科学技术出版社,2000: 58
[3] The summary of the sixth national conference on respiratory system of Chinese Medical Association[J]. Zhonghua Jie He He Hu Xi Za Zhi, 2001,24(1):8-11
中华医学会第六次全国呼吸系学术会议纪要[J]. 中华结核和呼吸杂志, 2001,24(1):8-11
[4] Lukacs NW, Lamm WJ, Strieter RM, Albert RK . Airway hyperreactivity is associated with specific leukocyte subset infiltration in a mouse model of allergic airway inflammation[J]. Pathobiology, 1996,64(6):308-313
doi: 10.1159/000164065
[5] Anticevich SZ, Hughes JM, Black JL, Armour CL . Induction of hyperresponsiveness in human airway tissue by neutrophils mechanism of action[J]. Clin Exp Allergy, 1996,26(5):549-556
doi: 10.1111/cea.1996.26.issue-5
[6] Park HS, Uh ST, Park CS . Increased neutrophil chemotactic activity is noted in alminum-induced occupational asthma[J]. Korean J Intern Med, 1996,11(1):69-73
doi: 10.3904/kjim.1996.11.1.69
[7] Tumer MO, Hussack P, Sears MR, Dolovich J, Hargreave FE . Exacerbations of asthma without sputum eosinophilia[J]. Thorax, 1995,50(10):1057-1061
doi: 10.1136/thx.50.10.1057
[8] The ENFUMOSA cross-sectional European multicentre study of the clinical phenotype of chronic severe asthma. European Network for Understanding Mecha-nisms of Severe Asthma[J]. Eur Respir J, 2003,22(3):470-477
doi: 10.1183/09031936.03.00261903
[9] Jatakanon A, Uasuf C, Maziak W, Lim S, Chung KF, Barnes PJ . Neutrophillic inflammation in severe persistent asthma[J]. Am J Respir Crit Care Med, 1999,160(5 Pt 1):1532-1539
doi: 10.1164/ajrccm.160.5.9806170
[10] Hussell T, Spender LC, Georgiou A, O'Garra A, Openshaw PJ .Th1 and Th2 cytokine induction in pulmonary T cells during infection with respiratory syncytial virus[J]. J Gen Virol, 1996,77(Pt 10):2447-2455
doi: 10.1099/0022-1317-77-10-2447
[1] Yi Ran Hu, San Li Xing, Chuan Chen, Ding Zhu Shen, Jiu Lin Chen. Tiaoxin Recipe, a Chinese herbal formula, inhibits microRNA-34a expression in the APPswe/PS1ΔE9 mouse model of Alzheimer's disease. Journal of Integrative Medicine, 2019, 17(6): 404-409.
[2] Oluwakemi Josephine Awakan, Sylvia Omonirume Malomo, Abdullahi Adeyinka Adejare, Adedoyin Igunnu, Olubunmi Atolani, Abiodun Humphrey Adebayo, Bamidele Victor Owoyele . Anti-inflammatory and bronchodilatory constituents of leaf extracts of Anacardium occidentale L. in animal models. Journal of Integrative Medicine, 2018, 16(1): 62-70.
[3] Bhesh Raj Sharma, Dong Wook Kim, Dong Young Rhyu. Korean Chungtaejeon tea extract attenuates weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2017, 15(1): 56-63.
[4] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[5] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[6] Md. Asadujjaman, Ahmed Ullah Mishuk, Md. Aslam Hossain, Utpal Kumar Karmakar. Medicinal potential of Passiflora foetida L. plant extracts: Biological and pharmacological activities. Journal of Integrative Medicine, 2014, 12(2): 121-126.
[7] Marjan Nassiri-Asl, Taghi Naserpour Farivar, Esmail Abbasi, Hamid Reza Sadeghnia, Mehdi Sheikhi, Mina Lotfizadeh, Parisa Bazahang. Effects of rutin on oxidative stress in mice with kainic acid-induced seizure. Journal of Chinese Integrative Medicine, 2013, 11(5): 337-342.
[8] Md. Khirul Islam, Imran Mahmud, Sanjib Saha, Asit Baron Sarker, Himangsu Mondal, A.S.M. Monjur-Al-Hossain, Md. Anisuzzman. Preliminary pharmacological evaluation of Alocasia indica Schott tuber. Journal of Chinese Integrative Medicine, 2013, 11(5): 343-351.
[9] Sanjib Saha, Faroque Hossain, Md. Anisuzzman, Md. Khirul Islam. Pharmacological evaluation of Musa seminifera Lour. Fruit. Journal of Chinese Integrative Medicine, 2013, 11(4): 253-261.
[10] Bolleddula Jayaprakasam, Nan Yang, Ming-Chun Wen, Rong Wang, Joseph Goldfarb, Hugh Sampson, Xiu-Min Li. Constituents of the anti-asthma herbal formula ASHMITM synergistically inhibit IL-4 and IL-5 secretion by murine Th2 memory cells, and eotaxin by human lung fibroblasts in vitro. Journal of Chinese Integrative Medicine, 2013, 11(3): 195-205.
[11] Sunil A. Nirmal, Jayashri M. Ingale, Shashikant R. Pattan, Sanjay B. Bhawar. Amaranthus roxburghianus root extract in combination with piperine as a potential treatment of ulcerative colitis in mice. Journal of Chinese Integrative Medicine, 2013, 11(3): 206-212.
[12] Lin Wang, Guan-liang Wang, Jia-han Liu, Di Li, De-zeng Zhu, Liang-neng Wu . Effects of ursolic acid in ameliorating insulin resistance in liver of KKAy mice via peroxisome proliferator-activated receptors α and γ. Journal of Chinese Integrative Medicine, 2012, 10(7): 793-799.
[13] Yi-tao Zhao , Xin-guang Zhang , Li Bai , Li-qing Li , Jian-er Yu . Effects of Chinese herbal medicine Pingchuan Formula on airway inflammation, interferon-γ and interleukin-4 in mice with asthma. Journal of Chinese Integrative Medicine, 2012, 10(7): 807-813.
[14] Mei-jiao Mao, Jun-ping Hu, Cong Wang, Yi-yi Zhang, Ping Liu. Effects of Chinese herbal medicine Guanxinkang on expression of PPARγ-LXRα-ABCA1 pathway in ApoE-knockout mice with atherosclerosis. Journal of Chinese Integrative Medicine, 2012, 10(7): 814-820.
[15] Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu. Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes. Journal of Chinese Integrative Medicine, 2012, 10(7): 821-826.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Yi-ting He, Qing-lin Zha, Jian-ping Yu, Yong Tan, Cheng Lu, Ai-ping Lv. Principal factor analysis of symptoms of rheumatoid arthritis and their correlations with efficacy of traditional Chinese medicine and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 32-36
[3] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[4] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[5] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[6] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[7] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[8] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[9] Guo-hong Yuan, Xiao-jing Pang, He-chao Ma. Synergic effects of Danggui Buxue Decoction in reducing toxicity of cytoxan in tumor-bearing mice. Journal of Chinese Integrative Medicine, 2008, 6(1): 83-88
[10] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013