中药复方别敏胶囊对呼吸道变应性小鼠内分泌免疫功能的调节作用

doi:10.3736/jcim20090409

Abstract

Objective

To investigate the effects of Biminne, a traditional Chinese herbal formula, in regulating the endocrine-immune function in mice with airway allergy.
Methods

Forty BALB/c mice were randomly divided into four groups: control group, untreated group, Biminne group, and dexamethasone (Dex) group. The murine model of airway allergy was established by intraperitoneal injection sensitization and intranasal challenge with ovalbumin (OVA). On day 28, the mice were sacrificed and the sera were collected. The lung tissue of mice was removed for haematoxylin-eosin staining and the pathological changes were observed. The spleen cells of mice were isolated and the ratio of Th2/Th1 was determined by flow cytometry. The isolated spleen cells were also cultured in 24-well plates in the presence of concanavalin A and the supernatants were collected after 72-hour culture. Levels of interleukin-4 (IL-4), IL-5 and interferon-gamma (IFN-γ) in the supernatants and the corticosterone level in sera were analyzed by enzyme-linked immunosorbent assay.
Results

Biminne significantly relieved the inflammatory reaction in the lung tissue of airway allergic mice. Compared with the control group, the Th2 cells in the untreated group increased, while the IFN-γ level decreased and the ratio of Th2/Th1 increased significantly, showing a Th2 response priority. Biminne treatment could down-regulate the Th2 cells and their cytokines IL-4 and IL-5, and up-regulate the Th1 cells and their cytokine IFN-γ. So the ratio of Th2/Th1 decreased significantly, shifting to the control level. In addition, Biminne also inhibited the reduction of the serum corticosterone of sensitized mice.
Conclusion

The Chinese herbal formula Biminne can reverse the functional disorder of the endocrine-immune system, which may explain its therapeutic effects on airway allergic disease.

Key words: Allergy, Interleukin-4, Interleukin-5, Interferon-gamma, Corticosterone, Mice

Su-qin Zhang, Zi-yin Shen, Guo-rang Hu, Run-hong Liu, Xin-ming Zhang. Effects of Chinese herbal formula Biminne in regulating endocrine-immune function in mice with airway allergy. Journal of Chinese Integrative Medicine, 2009, 7(4): 349-353.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20090409

http://www.jcimjournal.com/EN/Y2009/V7/I4/349

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References 12

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Group	n	EOS
Control	10	2.0±2.4
Untreated	10	19.0±6.4**
Dex	10	3.0±1.4^△△
Biminne	10	3.1±2.3^△△**

Group	n	Th2 cells (%)	Th1 cells (%)	Th2/Th1
Control	10	0.79±0.45	0.95±0.29	1.14±0.20
Untreated	10	1.47±0.56*	0.83±0.41	1.60±0.60*
Dex	10	0.91±0.51^△	0.68±0.46	1.29±0.45^△
Biminne	10	1.00±0.43^△	1.24±0.42^△	1.13±0.41^△

Group	n	IL-4 (ng/L)	IL-5 (ng/L)	INF-γ (ng/L)	IL-4/IFN-γ
Control	10	47.93±17.40	52.87±24.19	123.50±88.58	0.51±0.37
Untreated	10	50.50±13.49	55.36±44.00	42.80±17.49*	1.07±0.37**
Dex	10	30.90±13.64^△	30.78±9.82^△	46.58±34.69	0.87±0.64
Biminne	10	18.25±5.81^△	33.15±15.34^△	126.50±91.38^△	0.24±0.20^△△

Effects of Chinese herbal formula Biminne in regulating endocrine-immune function in mice with airway allergy

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