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Journal of Chinese Integrative Medicine ›› 2009, Vol. 7 ›› Issue (6): 532-540.doi: 10.3736/jcim20090608

• Original Experimental Research • Previous Articles     Next Articles

Effects of Bushen Kangshuai Tang in retrieving oxidative stress-induced reproductive defects in Caenorhabditis elegans

Xin-guo Cao, Li-li Hou, Jun-xia Chen, Qin Lu   

  1. Department of Traditional Chinese Medicine, Nanjing Maternity and Child Health Care Hospital, Nanjing Medical University, Nanjing 210004, Jiangsu Province, China
  • Received:2008-09-22 Accepted:2009-04-03 Online:2009-06-20 Published:2009-06-15


To explore the function of Bushen Kangshuai Tang (BKT), a compound traditional Chinese herbal medicine, in alleviating oxidative stress-induced reproductive defects in organism nematode Caenorhabditis elegans.

The L4-larvae were cultured with 25%, 50%, 75%, and 100% of BKT with the final concentration of 0.33 g/mL. Ultraviolet irradiation [20 J/(m 2·min)], heat-shock (36 ℃, 2 h), and paraquat treatment (2 mmol/L, 2 h) were exerted as the stresses. Reproduction was assayed by the egg number in uterus, brood size, and generation time.

Ultraviolet irradiation, heat-shock, and paraquat treatment could significantly reduce egg number in uterus and brood size, increase generation time, and suppress activities of catalase and superoxide dismutase of the treated wild-type N2 nematodes. Administration of BKT did not cause toxic or altered effects on reproduction under wild-type background in C. elegans. However, BKT administration at higher concentrations could not only effectively alleviate the reproductive defects induced by ultraviolet irradiation, heat-shock, and paraquat treatment, but also increase the catalase and superoxide dismutase activities suppressed by ultraviolet irradiation, heat-shock, and paraquat treatment as compared with the control. Moreover, administration of BKT at higher concentrations could largely ecover the reproductive defects formed in mev-1 mutant nematodes with elevated oxidative stress.

Oxidative stress can negatively regulate the reproductive process, and the administration of high concentrations of BKT will largely retrieve the oxidative stress-induced reproductive defects in exposed nematodes.

Key words: Bushen Kangshuai Tang, Oxidative stress, Reproduction, Caenorhabditis elegans

Figure 1

Effects of BKT administration on egg number in uterus, brood size and generation time in wild-type N2 nematodes Exposures were performed for 2 h on L4-larva stage nematodes with 25%, 50%, 75%, and 100% of examined BKT diluted with M9 buffer. At least 20 replicates were performed for assay of egg number in uterus, brood size and generation time for statistical purposes. One-way ANOVA followed by a Dunnett's t test was used to determine the significance of the differences between the groups. Data are expressed as x±s, n=30."

Figure 2

Retrieval effects of BKT administration on egg number in uterus, brood size and generation time in UV-irradiated wild-type N2 nematodes Approximately 30 L2-stage larvae were irradiated on NGM plates without food at 20 J/(m2·min). All UV irradiation assays were performed at 20 ℃, and afterwards further maintained at 20 ℃. Exposures were performed for 2 h on L4-larva stage nematodes with 25%, 50%, 75%, and 100% of examined BKT diluted with M9 buffer. All assays were replicated more than three times. Data are expressed as x±s, n=30. *P<0.05, **P<0.01, vs control group; △P<0.05, △△P<0.01, vs UV group."

Figure 3

Retrieval effects of BKT administration on egg number in uterus, brood size and generation time in heat-shock treated wild-type N2 nematodes HS, heat-shock. Approximately 30 L2-stage larvae grown on 20 ℃ were heat stressed for 2 h at 36 ℃ and afterwards further maintained at 20 ℃. Exposures were performed for 2 h on L4-larva stage nematodes with 25%, 50%, 75%, and 100% of examined BKT. All assays were replicated more than three times. Data are expressed as x±s, n=30. *P<0.05, **P<0.01, vs control group; ▲P<0.05, ▲▲P<0.01, vs HS group."

Figure 4

Retrieval effects of BKT administration on egg number in uterus, brood size and generation time in paraquat-treated wild-type N2 nematodes Approximately 30 L2-stage larvae were treated with 2 mmol/L paraquat solution for 2 h and survival at 20 ℃. Exposures were performed for 2 h on L4-larva stage nematodes with 25%, 50%, 75%, and 100% of examined BKT diluted with M9 buffer. All assays were replicated more than three times. Data are expressed as x±s, n=30. *P<0.05, **P<0.01, vs control group; □P<0.05, □□P<0.01, vs paraquat group."

Figure 5

Effects of BKT administration on CAT and SOD activities of UV-irradiated, heat-shock- and paraquat-treated wild-type N2 nematodes HS, heat-shock. Approximately 30 L2-stage larvae were irradiated on NGM plates without food at 20 J/(m2·min) at 20 ℃, or heat stressed for 2 h at 36 ℃, or treated with 2 mmol/L paraquat solution for 2 h and survival at 20 ℃. Exposures were performed for 2 h on L4-larva stage nematodes with 25%, 50%, 75%, and 100% of examined BKT diluted with M9 buffer. All assays were replicated more than three times. Data are expressed as x±s, n=30. *P<0.05, **P<0.01, vs control group; △P<0.05, △△P<0.01, vs UV group; ▲P<0.05, ▲▲P<0.01, vs HS group; □P<0.05, □□P<0.01, vs paraquat group."

Figure 6

Administration of BKT could retrieve the defects of egg number in uterus, brood size and generation time in mev-1 mutant nematodes N2, wild-type N2 nematodes. Exposures were performed for 2 h on L4-larva stage nematodes with 25%, 50%, 75%, and 100% of examined BKT diluted with M9 buffer. All assays were replicated more than three times. Data are expressed as x±s, n=30. *P<0.05, **P<0.01, vs control group; ■P<0.05, ■■P<0.01, vs mev-1 group."

[1] Moberg GP . How behavioral stress disrupts the endocrine control of reproduction in domestic animals[J]. J Dairy Sci, 1991,74(1)
[2] Jordan ER . Effects of heat stress on reproduction[J]. J Dairy Sci, 2003,86(Suppl):E104-E114
doi: 10.3168/jds.S0022-0302(03)74043-0
[3] Tilbrook AJ, Turner AI, Clarke IJ . Effects of stress on reproduction in non-rodent mammals: the role of glucocorticoids and sex differences[J]. Rev Reprod, 2000,5(2):105-113
doi: 10.1530/ror.0.0050105
[4] Dobson H, Ghuman S, Prabhakar S, Smith R . A conceptual model of the influence of stress on female reproduction[J]. Reproduction, 2003,125(2):151-163
doi: 10.1530/rep.0.1250151
[5] Maeda K, Tsukamura H . The impact of stress on reproduction: Are glucocorticoids inhibitory or protective to gonadotropin secretion?[J]. Endocrinology, 2006,147(3):1085-1086
doi: 10.1210/en.2005-1523
[6] Wang Y, Xie W, Wang DY . Transferable properties of multi-biological toxicity caused by cobalt exposure in Caenorhabditis elegans[J]. Environ Toxicol Chem, 2007,26(11):2405-2412
doi: 10.1897/06-646R1.1
[7] Desai C, Garriga G , McIntire SL, Horvitz HR.A genetic pathway for the development of the Caenorhabditis elegans HSN motor neurons[J]. Nature, 1988,336(6200):638-646
doi: 10.1038/336638a0
[8] Crittenden SL, Bernstein DS, Bachorik JL, Thompson BE, Gallegos M, Petcherski AG, Moulder G, Barstead R, Wickens M, Kimble J . A conserved RNA-binding protein controls germline stem cells in Caenorhabditis elegans[J]. Nature, 2002,417(6889):660-663
doi: 10.1038/nature754
[9] Kimble JE, White JG . On the control of germ cell development in Caenorhabditis elegans[J]. Dev Biol, 1981,81(2):208-219
doi: 10.1016/0012-1606(81)90284-0
[10] Coohill T, Marshall T, Schubert W, Nelson G . Ultraviolet mutagenesis of radiation-sensitive(rad) mutants of the nematode Caenorhabditis elegans[J]. Mutat Res, 1988,209(3-4):99-106
doi: 10.1016/0165-7992(88)90025-5
[11] Lithgow GJ, White TM, Hinerfeld DA, Johnson TE . Thermotolerance of a long-lived mutant of Caenorhabditis elegans[J]. J Gerontol, 1994,49(6):B270-B276
doi: 10.1093/geronj/49.6.B270
[12] Bojjawar T, Jalari M, Aamodt E, Ware MF, Haynie DT . Effect of electromagnetic nanopulses on C. elegans fertility[J]. Bioelectromagnetics, 2006,27(7):515-520
doi: 10.1002/(ISSN)1521-186X
[13] Wang DY, Yang P . The multi-biological defects caused by lead exposure exhibit transferable properties from exposed parents to their progeny in Caenorhabditis elegans[J]. J Environ Sci, 2007,19(11):1367-1372
doi: 10.1016/S1001-0742(07)60223-X
[14] Wang DY, Yang P . Silver exposure causes transferable defects of phenotypes and behaviors in nematode Caenorhabditis elegans[J]. Environ Bioindic, 2007,2(2):89-98
doi: 10.1080/15555270701457695
[15] Wang D, Wang Y . Nickel sulfate induces numerous defects in Caenorhabditis elegans that can also be transferred to progeny[J]. Environ Pollut, 2008,151(3):585-592
doi: 10.1016/j.envpol.2007.04.003
[16] Wang DY, Shen LL, Wang Y . The phenotypic and behavioral defects can be transferred from zinc exposed nematodes to their progeny[J]. Environ Toxicol Pharmacol, 2007,24(3):223-230
doi: 10.1016/j.etap.2007.05.009
[17] Brenner S . The genetics of Caenorhabditis elegans[J]. Genetics, 1974,77(1):71-94
[18] Donkin SG, Williams PL . Influence of developmental stage, salts and food presence on various end points using Caenorhabditis elegans for aquatic toxicity testing[J]. Environ Toxicol Chem, 1995,14(11):2139-2147
doi: 10.1897/1552-8618(1995)14[2139:IODSSA]2.0.CO;2
[19] Williams PL, Dusenbery DB . Aquatic toxicity testing using the nematode Caenorhabditis elegans[J]. Environ Toxicol Chem, 1990,9(3):1285-1290
doi: 10.1897/1552-8618(1990)9[1285:ATTUTN]2.0.CO;2
[20] Murakami S, Johnson TE . A genetic pathway conferring life extension and resistance to UV stress in Caenorhabditis elegans[J]. Genetics, 1996,143(3):1207-1218
[21] Wang DY, Wang Y . Phenotypic and behavioral defects caused by barium exposure in nematode Caenorhabditis elegans[J]. Arch Environ Contam Toxicol, 2008,54(3):447-453
doi: 10.1007/s00244-007-9050-0
[22] Abei H . Catalase in vitro[J]. Meth Enzymol, 1984,105(2):121-126
doi: 10.1016/S0076-6879(84)05016-3
[23] Cypser JR, Johnson TE . The spe-10 mutant has longer life and increased stress resistance[J]. Neurobiol Aging, 1999,20(5):503-512
doi: 10.1016/S0197-4580(99)00085-8
[24] Ye H, Ye B, Wang D . Trace administration of vitamin E can retrieve and prevent UV-irradiation- and metal exposure-induced memory deficits in nematode Caenorhabditis elegans[J]. Neurobiol Learn Mem, 2008,90(1):10-18
doi: 10.1016/j.nlm.2007.12.001
[25] Springer W, Hoppe T, Schmidt E, Baumeister R . A Caenorhabditis elegans Parkin mutant with altered solubility couples alpha-synuclien aggregation to proteotoxic stress[J]. Hum Mol Genet, 2005,14(22):3407-3423
doi: 10.1093/hmg/ddi371
[26] Sampayo JN, Olsen A, Lithgow GJ . Oxidative stress in Caenorhabditis elegans: protective effects of superoxide dismutase/catalase mimetics[J]. Aging Cell, 2003,2(6):319-326
doi: 10.1046/j.1474-9728.2003.00063.x
[27] Halliwell B, Gutteridge JMC. Free radicals in biology and medicine(3rd ed)[M]. Oxford: Oxford University Press,1999,(3rd ed)
[28] Duong TT, Antao S, Ellis NA, Myers SJ, Witting PK . Supplementation with a synthetic polyphenol limits oxidative stress and enhances neuronal cell viability in response to hypoxia-re-oxygenation injury[J]. Brain Res, 2008,( 1219):8-18
[29] Ansari MA, Roberts KN, Scheff SW . Oxidative stress and modification of synaptic proteins in hippocampus after traumatic brain injury[J]. Free Radic Biol Med, 2008,45(4):443-452
doi: 10.1016/j.freeradbiomed.2008.04.038
[30] Lin YT, Hoang H, Hsieh SI, Rangel N, Foster AL, Sampayo JN, Lithgow GJ, Srinivasan C . Manganousion supplementation accelerates wild type development, enhances stress resistance, and rescues the life span of a short-lived Caenorhabditis elegans mutant[J]. Free Radic Biol Med, 2006,40(7):1185-1193
doi: 10.1016/j.freeradbiomed.2005.11.007
[31] Ishii N . Oxidative stress and aging in Caenorhabditis elegans[J]. Free Radic Res, 2000,33(6):857-864
doi: 10.1080/10715760000301371
[32] Melov S, Ravenscroft J, Malik S, Gill MS, Walker DW, Clayton PE, Wallace DC, Malfroy B, Doctrow SR, Lithgow GJ . Extension of life-span with superoxide dismutase/catalase mimetics[J]. Science, 2000,289(5484):1567-1569
doi: 10.1126/science.289.5484.1567
[33] Yu X, Kamijima M, Ichihara G, Li W, Kitoh J, Xie Z, Shibata E, Hisanaga N, Takeuchi Y . 2-Bromopropane causes ovarian dysfunction by damaging primordial follicles and their oocytes in female rats[J]. Toxicol Appl Pharmacol, 1999,159(3):185-193
doi: 10.1006/taap.1999.8730
[34] Mattison DR . Morphology of oocyte and follicle destruction by polycyclic aromatic hydrocarbons in mice[J]. Toxicol Appl Pharmacol, 1980,53(2):249-259
doi: 10.1016/0041-008X(80)90424-X
[35] Forges T, Monnier-Barbarino P, Leheup B, Jouvet P . Pathophysiology of impaired ovarian function in galactosaemia[J]. Hum Reprod Update, 2006,12(5):573-584
doi: 10.1093/humupd/dml031
[36] Fisher AL, Page KE, Lithgow GL, Nash L . The Caenorhabditis elegans K10C2.4 gene encodes a member of the fumarylacetoacetate hydrolase family: a Caenorhabditis elegans model of type Ⅰ tyrosinemia[J]. J Biol Chem, 2008,283(14):9127-9135
doi: 10.1074/jbc.M708341200
[37] Salinas LS, Maldonado E, Navarro RE . Stress-induced germ cell apoptosis by a p53 independent pathway in Caenorhabditis elegans[J]. Cell Death Differ, 2006,13(12):2129-2139
doi: 10.1038/sj.cdd.4401976
[38] DeCorby A, Gàskovà D, Salves LC, Lemire BD . Expression of Ndi 1p, an alternative NADH: ubiquinone oxidoreductase, increase mitochondrial membrane potential in a C. elegans model of mitochondrial disease[J]. Biochem Biophys Acta, 2007,1767(9):1157-1163
[39] Conway GS . Premature ovarian failure[J]. Brit Med Bull, 2000,56(3):643-649
doi: 10.1258/0007142001903445
[40] Goswami D, Conway GS . Premature ovarian failure[J]. Hum Reprod Update, 2005,11(4):391-410
doi: 10.1093/humupd/dmi012
[41] Hu XM, Roberts JR, Apopa PL, Kan YW, Ma Q . Accelerated ovarian failure induced by 4-vinyl cyclohexene diepoxide in Nrf2 null mice[J]. Mol Cell Biol, 2006,26(3):940-954
doi: 10.1128/MCB.26.3.940-954.2006
[42] Graziewicz MA, Bienstock RJ, Copeland WC . The DNA polymerase γ Y955C disease variant associated with PEO and parkinsonism mediates the incorporation and translesion synjournal opposite 7, 8-dihydro-8-oxo-2'-deoxyguanosine[J]. Hum Mol Genet, 2007,16(22):2729-2739
doi: 10.1093/hmg/ddm227
[43] Chen LL, Wu C, Li W . Study on antioxidative activity of flavonoids from Semen Cuscutae[J]. Shi Pin Gong Ye Ke Ji, 2008,29(11):116-117, 121
陈林林, 吴春, 李伟 . 菟丝子黄酮的抗氧化性能研究[J]. 食品工业科技, 2008,29(11):116-117, 121
[44] 安红梅, 史云峰, 胡兵, 史秀峰, 张占鹏, 许丽雯, 顾明昌 . 熟地黄对D-半乳糖衰老模型大鼠脑衰老的作用研究[J]. 中药药理与临床, 2008,24(3):59-60
An HM, Shi YF, Hu B, Shi XF, Zhang ZP, Xu LW, Gu MC.Prepared Radix Rehmanniae( 熟地黄) delay brain senescence in D-galactose induced senile rats[J]. Zhong Yao Yao Li Yu Lin Chuang, 2008,24(3):59-60
[45] Wu HH, Xuan GD, Liu CQ, Hu QH . Study on purification and antioxidant activity of phenylethanoid glycosides from Cistanche salsa[J]. Shi Pin Ke Xue, 2008,29(6):190-193
吴海虹, 玄国东, 刘春泉, 胡秋辉 . 肉苁蓉苯乙醇苷的纯化及其抗氧化活性研究[J]. 食品科学, 2008,29(6):190-193
[46] Li Y, Su QQ . Study on antioxidant and antimicrobial activity of Morinda officinalis How water extract[J]. Shi Pin Yu Ji Xie, 2008,24(1):93-95
李妍, 苏倩清 . 巴戟天水提取物油脂抗氧化性和抗菌活性研究[J]. 食品与机械, 2008,24(1):93-95
[47] Liu W, Liu CH, Yu HQ . Effect of Chuanxiong and Danggui on Na+ and K+ currents of rat hippocampal neurons with hypoxia-reoxygenation[J]. Zhongguo Lin Chuang Kang Fu, 2006,10(7):162-164
刘卫, 刘传缋, 俞惠琴 . 中药川芎、当归有效成分对缺氧后复氧大鼠海马神经元钠、钾电流的作用[J]. 中国临床康复, 2006,10(7):162-164
[48] Huang F, Xu LJ, Du GH, Xiao PG . In vitro antioxidative and cytotoxic activities of Schisandra sphenanthera Rehd. et Wils[J]. Tian Ran Chan Wu Yan Jiu Yu Kai Fa, 2006,18(1):85-87
黄锋, 许利嘉, 杜冠华, 肖培根 . 华中五味子抗氧化和细胞毒活性研究[J]. 天然产物研究与开发, 2006,18(1):85-87
[49] Letur-Konirsch H, Delanian S . Successful pregnancies after combined pentoxifylline-tocopherol treatment in women with premature ovarian failure who are resistant to hormone replacement therapy[J]. Fertil Steril, 2003,79(2):439-441
doi: 10.1016/S0015-0282(02)04579-X
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