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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (6): 575-580.doi: 10.3736/jcim20100611

• Original Experimental Research • Previous Articles     Next Articles

Effects of Tenglong Buzhong Decoction on proliferation and apoptosis of human colon carcinoma cell line LS174T

Bing Hua,  Hong-mei Anb,Ke-ping Shenc,Qin Dua   

  1. a No. 5 Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    b E-institute of Internal Medicine of Traditional Chinese Medicine, Shanghai Municipal Education Commission, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    c Department of Science & Technology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China;
  • Received:2010-01-06 Accepted:2010-03-31 Online:2010-06-20 Published:2010-06-15


To observe the effects of Tenglong Buzhong Decoction (TLBZD), a compound traditional Chinese herbal medicine, on proliferation and apoptosis of colon carcinoma cell line LS174T in vitro.

Human colon carcinoma cell line LS174T and human colon epithelial cell line CRL-1790 were treated with different doses of TLBZD. Cell proliferation was detected with cell counting kit-8 (CCK-8) assay and clone formation assay. Cell cycle and apoptosis were detected by flow cytometry, and caspase-3, -8 and -9 activities in LS174T cells were detected by colorimetric assay.

TLBZD had no obvious cytotoxicity in normal CRL-1790 cells. After 72-hour treatment of 1 mg/mL TLBZD, or 48- and 72-hour of 2 mg/mL TLBZD, or 24-, 48- and 72-hour of 5-20 mg/mL TLBZD, proliferation of LS174T cells was significantly inhibited. Clone formation of LS174T cells was significantly inhibited by 1 to 20 mg/mL TLBZD treatment. TLBZD at doses of 5 to 20 mg/mL also induced apoptosis and cell cycle arrest at G0/G1 phase in LS174T cells. In addition, caspase-3, -8 and -9 activities were significantly elevated after 5 to 20 mg/mL TLBZD treatment.

TLBZD can inhibit cell proliferation, arrest cell cycle at G0/G1 phase, and induce apoptosis in LS174T cells, which may be related to activating of caspase-3, -8 and -9.

Key words: Colon neoplasm, Chinese medical formula, Cell proliferation, Apoptosis, Caspases, In vitro, LS174T cells




Figure 1

Effects of TLBZD on cell cycle and apoptosis of LS174T cells"


Figure 2

Effects of TLBZD on caspase activities of LS174T cellsData were represented as x±s, n=3. **P<0.01, vs control group."

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