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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (6): 541-547.doi: 10.3736/jcim20100606

• Original Experimental Research • Previous Articles     Next Articles

Optimal combination of baicalin, icariin and Astragalus saponin Ⅰ from a Chinese herbal compound Biminne

 Wei-yi Gonga, Xin-min Zhanga,Zi-yin Shena,Guo-rang Hub, Su-qin Zhanga,  Run-hong Liua, Wei-dong Zhangc, Jing-cheng Donga   

  1. a Institute of Chinese Integrative Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China
    b Department of Immunology and Allergy, Concord Hospital, Sydney University, Sydney 2006, Australia
    c School of Pharmacy, Second Military Medical University, Shanghai 200433, China
  • Received:2010-02-05 Accepted:2010-03-31 Online:2010-06-20 Published:2010-06-15
  • Contact: Xin-min Zhang E-mail:xmzhang54@yahoo.com

Objective

To study the optimal combined ratio of baicalin, icariin and Astragalus saponin Ⅰ from a Chinese herbal compound Biminne.
Methods

Firstly, a mouse model of allergic rhinitis was established by intraperitoneal injection of ovalbumin (OVA) and aluminum hydroxide gel suspension, and the effective dose range of baicalin, icariin and Astragalus saponin Ⅰ was detected by 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt method. Secondly, 10 groups of combinations of baicalin, icariin and Astragalus saponin Ⅰ assembled by U*10(108) form were employed to determine the optimal combination by means of analyzing of the inhibitory effect on the splenocyte proliferation. Finally, the effects of each effective ingredient and the optimal combination were compared by observing the splenocyte proliferation, the contents of interleukin-4 (IL-4), IL-5, interferon-gamma (IFN-γ) in supernatant of the splenocyte cultures and the ratio of IL-4 to IFN-γ in order to verify the result.
Results

Baicalin or icariin at concentrations ranging from 2 to 10 μmol/L, and Astragalus saponin Ⅰ from 1 to 10 μmol/L effectively suppressed the splenocyte proliferation. When the proportion of baicalin, icariin and Astragalus saponin Ⅰ was 1∶2.14∶2.65, the inhibitory effect was most remarkable. Further research confirmed the rationality of the optimal combination.
Conclusion

An optimal combination of the major effective ingredients from Chinese herbal compound Biminne most effectively suppresses the proliferation of splenocytes from sensitized mice and regulates the cytokine secreting.

Key words: Chinese herbal compound, Allergic rhinitis, Cell proliferation, Compatibility, Uniform design, Lymphocytes

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Figure 1

Effective dose range of baicalin inhibiting the splenocyte proliferationData were represented as x±s, n=5. **P<0.01, vs ConA group."

Figure 2

Effective dose range of icariin inhibiting the splenocyte proliferation Data were represented as x±s, n=5. **P<0.01, vs ConA group."

Figure 3

Effective dose range of Astragalus saponin Ⅰ inhibiting the splenocyte proliferation Data were represented as x±s, n=5. **P<0.01, vs ConA group."

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Figure 4

Effects of baicalin, icariin, Astragalus saponin Ⅰ and the combination on splenocyte proliferation A: Control group; B: ConA group; C: Baicalin group; D: Icariin group; E: Astragalus saponin Ⅰ group; F: Combination group. Data were represented as x±s, n=5. *P<0.05, **P<0.01, vs ConA group; △△P<0.01, vs control group; ▲P<0.05, ▲▲P<0.01, vs combination group."

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