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Journal of Chinese Integrative Medicine ›› 2011, Vol. 9 ›› Issue (5): 558-564.doi: 10.3736/jcim20110515

• Original Experimental Research • Previous Articles     Next Articles

Antiproliferative effects of essential oil of a compound Chinese herbal medicine Weiqi Decoction on AGS cells

Bao Tan1, Hai-lian Shi1,2,3, Guang Ji1, Lan Lu2,3, Ai-li Cao2,3, Song-shan Shi2, Jian-qun Xie1()   

  1. 1. Department of Gastroenterology, Institute of Digestive Diseases, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    2. Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
    3. Shanghai Key Laboratory of Complex Prescription, Shanghai 201203, China
  • Received:2010-10-12 Accepted:2010-12-27 Online:2011-05-20 Published:2011-05-15
  • Contact: Xie Jian-qun E-mail:xiejq01@gmail.com

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Objective: The main ingredients and the inhibitory effects of essential oil of a compound Chinese herbal medicine Weiqi Decoction (WQD) on AGS cell proliferation were to be investigated.
Methods: Chemical compounds of WQD essential oil were detected by gas chromatography and mass spectrometry analysis. Cell viability was measured by methyl thiazolyl tetrazolium method. Cell cycle distribution was detected by flow cytometry. Apoptosis and necrosis of AGS cells were determined by Hoechst 33342/propidium iodine staining.
Results: Chemical analysis showed that the main ingredients of WQD essential oil were bornylene and 3-n-butylphthalide. Ligustilide, which is the effective compound of Danggui (Radix Angelicae Sinensis), was not detected in WQD essential oil. The essential oil inhibited cell proliferation in a dose- and time-dependent manner, and blocked cell cycle progression at G2/M stage. At the concentrations that resulted in significant inhibition of cell proliferation and cell cycle arrest, essential oil induced both apoptosis and necrosis.
Conclusion: The results suggest that WQD essential oil contains some effective ingredients for treating chronic atrophic gastritis and functional dyspepsia, and also has an antiproliferative effect on AGS cells through cell cycle arrest and apoptosis promotion in vitro. Therefore, essential oil should be retained as much as possible during stewing this decoction.

Key words: compound,traditional Chinese medicine, Weiqi Decoction, oils,volatile, gastritis,atrophic, AGS cells, cell proliferation

Figure 1

Gas chromatography and mass spectrometry analysis chromatogram of Weiqi Decoction essential oil The X axis represents the time when compounds appeared, and the Y axis represents the relative abundance of different compounds contained in Weiqi Decoction essential oil."

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Figure 2

AGS cell cycle phase distribution tested by flow cytometry"

Figure 3

Apoptosis and necrosis of AGS cells incubated without or with Weiqi Decoction essential oil for 12 h The blue color represents Hoechst 33342 which can penetrate the membrane and stain the DNA, and the red color represents propidium iodide which can also stain the DNA but cannot penetrate the membrane."

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