Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (1): 80-85.doi: 10.3736/jcim20100115

• Original Experimental Research • Previous Articles     Next Articles

Effects of a glucocorticoid on development of kidney deficiency syndrome in a rat model of asthma

 Pei Wanga, Li-ping Xua, Xi-hong Jinb,Jing Yanga,Ren-hui Liua,Xiu-juan Wanga   

  1. a School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
    b Department of Tuina, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
  • Received:2009-08-28 Accepted:2009-11-22 Online:2010-01-20 Published:2010-01-15
  • Contact: Ren-hui Liu ,Xiu-juan Wang E-mail:liurenhui995@163.com;wxj0517@sina.com

Objective

To observe the effects of dexamethasone (DXM) on changes of syndrome and hypothalamic-pituitary-adrenal (HPA) axis of asthmatic rats at different stages of intervention (before withdrawal, during withdrawal and after withdrawal).
Methods

Asthma was induced in rats by inhalation of 1% ovalbumin. Rats in DXM group were treated with different concentrations of DXM by intra-abdominal injection. At the different stages of DXM treatment (before withdrawal, during withdrawal and after withdrawal), symptoms of the rats were observed to differentiate the syndromes according to yin-deficiency and yang-deficiency measurement standard. Content of serum corticosterone (CORT) was detected by enzyme-linked immunosorbent assay, and contents of plasma adrenocorticotropic hormone (ACTH) and bone Gla-protein (BGP), and content of corticotropin-releasing hormone (CRH) in hypothalamic homogenate were detected by radioimmunoassay.
Results

Rats in the DXM group were differentiated as yin-deficiency syndrome before the stage of hormone withdrawal. During the period of hormone withdrawal and after the withdrawal, syndromes of rats in the DXM group accorded with the standards of yin- and yang-deficiency. During the period of treatment with DXM, contents of CORT, ACTH and CRH were significantly decreased, but recovered after DXM withdrawal. BGP level in the DXM group was lower than those in the normal group and the model group during all the stages.
Conclusion

Asthmatic rats treated with DXM have different syndrome characteristics in different stages. Kidney deficiency will be caused by DXM treatment.

Key words: Asthma, Glucocorticoid, Syndrome, Hypothalamic-pituitary-adrenal axis, Bone Gla-protein, Rats

Table 1

Body weight in rats (x±s, g)"

Group Body weight
1st day 42nd day 49th day 63rd day 77th day 91st day
Normal 129.34±7.78 372.41±35.11 374.90±37.95 434.70±47.05 450.00±49.15 496.60±42.58
Model 129.04±8.64 368.19±29.52 370.11±34.60 425.22±29.24 448.11±37.26 484.89±20.88
DXM 128.16±9.75 297.04±26.61**△△ 266.24±22.16**△△ 271.53±27.41**△△ 285.53±32.45**△△ 357.16±39.56**△△

Table 2

Degree of yin-deficiency in rats (x±s)"

Group n Degree of yin-deficiency
Normal
42nd day (before withdrawal) 29 1.00±0.09
49th day (before withdrawal) 29 1.00±0.10
63rd day (during withdrawal) 20 1.00±0.11
77th day (during withdrawal) 20 1.00±0.11
91st day (after withdrawal) 10 1.00±0.09
Model
42nd day (before withdrawal) 27 0.99±0.08
49th day (before withdrawal) 27 0.99±0.09
63rd day (during withdrawal) 18 0.98±0.07
77th day (during withdrawal) 18 1.00±0.08
91st day (after withdrawal) 9 0.98±0.04
DXM
42nd day (before withdrawal) 25 0.80±0.07**△△
49th day (before withdrawal) 25 0.71±0.06**△△
63rd day (during withdrawal) 17 0.62±0.06**△△
77th day (during withdrawal) 17 0.63±0.07**△△
91st day (after withdrawal) 8 0.72±0.08**△△

Table 3

Level of exuberance or deficiency of yang in rats (x±s)"

Group n Level of exuberance or deficiency of yang
Normal
42nd day (before withdrawal) 29 1.00±0.05
49th day (before withdrawal) 29 1.00±0.03
63rd day (during withdrawal) 20 1.00±0.02
77th day (during withdrawal) 20 1.00±0.02
91st day (after withdrawal) 10 1.00±0.04
Model
42nd day (before withdrawal) 27 1.00±0.03
49th day (before withdrawal) 27 1.02±0.04*
63rd day (during withdrawal) 18 1.00±0.02
77th day (during withdrawal) 18 1.01±0.04
91st day (after withdrawal) 9 0.96±0.02*
DXM
42nd day (before withdrawal) 25 1.03±0.03*△△
49th day (before withdrawal) 25 1.01±0.04
63rd day (during withdrawal) 17 1.00±0.02
77th day (during withdrawal) 17 0.97±0.04**△△
91st day (after withdrawal) 8 0.95±0.02**

Table 4

Contents of CORT, ACTH, CRH and BGP before withdrawal (x±s)"

Group n CORT (ng/L) ACTH (ng/L) CRH (ng/mg prot) BGP (ng/mL)
Normal 1 9 2 304.60±628.44 62.75±17.45 5.77±2.01 5.22±2.14
Model 1 9 1 658.27±485.60 32.66±17.21** 4.20±1.87* 3.64±1.40
DXM 1 8 235.20±160.17**△△ 19.67±8.82**△△ 3.40±1.28** 0.65±0.62**△△

Table 5

Contents of CORT, ACTH, CRH and BGP during withdrawal (x±s)"

Group n CORT (ng/L) ACTH (ng/L) CRH (ng/mg prot) BGP (ng/mL)
Normal 2 10 2 432.88±378.96 65.33±31.01 5.05±1.62 2.63±0.96
Model 2 9 1 905.06±410.87** 38.41±11.72 4.15±1.39 2.50±1.20
DXM 2 9 355.25±239.28**△△ 39.62±14.93 3.04±1.37** 0.79±0.53 **△△

Table 6

Contents of CORT, ACTH, CRH and BGP after withdrawal (x±s)"

Group n CORT (ng/L) ACTH (ng/L) CRH (ng/mg prot) BGP (ng/mL)
Normal 3 10 2 627.22±508.46 62.96±33.48 5.39±1.40 4.58±2.19
Model 3 9 2 318.42±364.32 48.79±19.90 3.80±0.94** 3.21±1.34
DXM 3 8 2 211.88±400.01* 30.67±19.14** 3.67±1.09** 1.93±0.91*△△
[1] Zhang TX . Systemic side effects of inhaled corticosteroids on children with asthma[J]. Lin Chuang Er Ke Za Zhi, 2004,22(10):703-704
张廷熹 . 哮喘儿童吸入糖皮质激素的全身副作用[J]. 临床儿科杂志, 2004,22(10):703-704
[2] Barnes PJ, Woolcock AJ . Difficult asthma[J]. Eur Respir J, 1998,12(5):1209-1218
doi: 10.1183/09031936.98.12051209
[3] Adcock IM, Lane SJ . Corticosteroid-insensitive asthma: molecular mechanisms[J]. J Endocrinol, 2003,178(3):347-355
doi: 10.1677/joe.0.1780347 pmid: 12967328
[4] Wu H, Shi SF . Research progress of traditional Chinese medicine in prevention and treatment of on hormone-dependent asthma[J]. Jiangxi Zhong Yi Yao, 2006,37(10):59-60
doi: 10.3969/j.issn.0411-9584.2006.10.039
吴寒, 史锁芳 . 激素依赖性哮喘中医药防治研究进展[J]. 江西中医药, 2006,37(10):59-60
doi: 10.3969/j.issn.0411-9584.2006.10.039
[5] Liu RH, Guo X . Experimental study on the adjustment of Peibenfang on disequilibrium of Th1/Th2 in asthma rat[J]. Shandong Zhong Yi Yao Dao Xue Xue Bao, 2008,32(5):423-425
刘仁慧, 郭忻 . 培本方调节哮喘大鼠Th1/Th2失衡作用的实验研究[J]. 山东中医药大学学报, 2008,32(5):423-425
[6] Liu XM, Fang ZQ, Lu WL, Pan ZQ, Shu S . Characteristic and development of syndrome about diabetes mellitus rats[J]. Liaoning Zhong Yi Za Zhi, 2008,35(1):130-134
刘小美, 方肇勤, 卢文丽, 潘志强, 舒适 . 糖尿病模型大鼠证候的特征及演变[J]. 辽宁中医杂志, 2008,35(1):130-134
[7] Lu WL, Fang ZQ, Pan ZQ, Fu XL . Comparison and evaluation of mouse models for four methods of examination in differentiation of eight syndromes such as qi, blood, yin or yang deficiency[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2006,12(6):433-438
doi: 10.3969/j.issn.1006-3250.2006.06.013
卢文丽, 方肇勤, 潘志强, 付晓玲 . 小鼠气、血、阴、阳、虚等八种模型四诊的比较和评价[J]. 中国中医基础医学杂志, 2006,12(6):433-438
doi: 10.3969/j.issn.1006-3250.2006.06.013
[8] Fang ZQ, Pan ZQ, Lu WL, Liu XM, Hou L, Liao MJ . Establishment and evaluation of metrological methods of syndrome differentiation about common syndrome in rats and mice[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2007,13(7):502-505
方肇勤, 潘志强, 卢文丽, 刘小美, 侯俐, 廖明娟 . 大鼠、小鼠常见证候计量化辨证方法的建立及其评价[J]. 中国中医基础医学杂志, 2007,13(7):502-505
[9] Cui Y, Zhou XS . Clinical observation of Guben Kechuan pill in treating patients with hormone-dependent bronchial asthma[J]. Zhongguo Zhong Yi Yao Ke Ji, 2001,8(3):189-190
崔悦, 周旭生 . 固本咳喘丸治疗激素依赖性支气管哮喘的临床观察[J]. 中国中医药科技, 2001,8(3):189-190
[10] Chen X, Fang ZQ . Change of neuroendocrine immune network in experimental animals with deficient syndrome[J]. Shanghai Zhong Yi Yao Da Xue Xue Bao, 2002,16(2):61-65
doi: 10.3969/j.issn.1008-861X.2002.02.021
陈晓, 方肇勤 . 神经内分泌免疫网络在虚证实验动物中的变化[J]. 上海中医药大学学报, 2002,16(2):61-65
doi: 10.3969/j.issn.1008-861X.2002.02.021
[11] Tong SH, Wu DX, Bao ZRGT, Chen SJ, Xi BL . Effects of Xiaoqinglong Decoction on levels of glucocorticoid hormone receptor and β-receptor in pulmonary tissues of a rat asthma model[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 1999,5(3):18-21
童舜华, 吴敦序 , 包照日格图, 陈淑俊, 奚蓓莉.小青龙汤对哮喘大鼠肺组织糖皮质激素受体和β-受体等水平的影响[J]. 中国中医基础医学杂志, 1999,5(3):18-21
[12] Peebles RS Jr, Togias A, Bickel CA, Diemer FB, Hubbard WC, Schleimer RP . Endogenous glucocorticoids and antigen-induced acute and late phase pulmo-nary responses[J]. Clin Exp Allergy, 2000,30(9):1257-1265
doi: 10.1046/j.1365-2222.2000.00890.x pmid: 10971472
[13] Yang QR, Yang LZ . Countermeasures of side effects and complications of corticosteroid[J]. Zhongguo Xian Dai Yi Sheng, 2008,46(3):31-32
doi: 10.3969/j.issn.1673-9701.2008.03.014
杨清儒, 杨璐舟 . 糖皮质激素的副作用及并发症对策[J]. 中国现代医生, 2008,46(3):31-32
doi: 10.3969/j.issn.1673-9701.2008.03.014
[14] Cui HS, Xu GX, Wang SR, Zhou GF . Effect of three-stage sequential therapy on the functions of hypothalamic-pituitary-adrenal axis and levels of the glucocorticoid receptor in patients with steroid-dependent asthma[J]. Zhonghua Zhong Yi Yao Za Zhi, 2008,23(11):961-964
崔红生, 徐光勋, 王硕仁, 周冠芬 . 三步序贯法对激素依赖型哮喘患者下丘脑-垂体-肾上腺皮质轴功能及糖皮质激素受体的影响[J]. 中华中医药杂志, 2008,23(11):961-964
[15] Shi CD, Jia WP . The effects of Kang Shu Jian Gu Granules on calcitonin and osteocalcin in osteoporostic rats caused by glucocorticoid[J]. Shannxi Zhong Yi Xue Yuan Xue Bao, 2008,31(4):59-60
史传道, 贾文鹏 . 抗疏健骨颗粒对骨质疏松模型大鼠降钙素和骨钙素的影响[J]. 陕西中医学院学报, 2008,31(4):59-60
[16] Meng ZH . Osteocalcin and its clinical significance[J]. Zhonghua Nei Fen Mi Dai Xie Za Zhi, 1992,8(1):41-44
孟昭亨 . 骨钙素及其临床意义[J]. 中华内分泌代谢杂志, 1992,8(1):41-44
[1] Sherman Gu, Arthur Yin Fan. Controversial conclusions from two randomized controlled trials for acupuncture's effects on polycystic ovary syndrome or in vitro fertilization support. Journal of Integrative Medicine, 2020, 18(2): 89-91.
[2] Xue-qing Yu, Shu-guang Yang, Yang Xie, Jian-sheng Li. Traditional Chinese medicine in the treatment of idiopathic pulmonary fibrosis based on syndrome differentiation: Study protocol of exploratory trial. Journal of Integrative Medicine, 2020, 18(2): 163-168.
[3] Rebecca Kulbida, Alexander Mathes, Johannes Loeser. Beneficial effects of hirudotherapy in a chronic case of complex regional pain syndrome. Journal of Integrative Medicine, 2019, 17(5): 383-386.
[4] Sonia Chew Wen Phang, Uma Devi Palanisamy, Khalid Abdul Kadir. Effects of geraniin (rambutan rind extract) on blood pressure and metabolic parameters in rats fed high-fat diet. Journal of Integrative Medicine, 2019, 17(2): 100-106.
[5] Mehranghiz Ebrahimi-Mameghani, Mohammad Asghari-Jafarabadi, Khatereh Rezazadeh. TCF7L2-rs7903146 polymorphism modulates the effect of artichoke leaf extract supplementation on insulin resistance in metabolic syndrome: A randomized, double-blind, placebo-controlled trial. Journal of Integrative Medicine, 2018, 16(5): 329-334.
[6] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[7] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[8] Oluwakemi Josephine Awakan, Sylvia Omonirume Malomo, Abdullahi Adeyinka Adejare, Adedoyin Igunnu, Olubunmi Atolani, Abiodun Humphrey Adebayo, Bamidele Victor Owoyele . Anti-inflammatory and bronchodilatory constituents of leaf extracts of Anacardium occidentale L. in animal models. Journal of Integrative Medicine, 2018, 16(1): 62-70.
[9] Chen Fu, Nevin Lianwen Zhang, Bao-xin Chen, Zhou Rong Chen, Xiang Lan Jin, Rong-juan Guo, Zhi-gang Chen, Yun-ling Zhang. Identification and classification of traditional Chinese medicine syndrome types among senior patients with vascular mild cognitive impairment using latent tree analysis. Journal of Integrative Medicine, 2017, 15(3): 186-200.
[10] Qian-wen Ma, Yong Tan. Effectiveness of co-treatment with traditional Chinese medicine and letrozole for polycystic ovary syndrome: A meta-analysis. Journal of Integrative Medicine, 2017, 15(2): 95-101.
[11] Jin Yu, Chao-qin Yu, Qi Cao, Li Wang, Wen-jun Wang, Li-rong Zhou, Jing Li, Qiao-hong Qian. Consensus on the integrated traditional Chinese and Western medicine criteria of diagnostic classification in polycystic ovary syndrome (draft). Journal of Integrative Medicine, 2017, 15(2): 102-109.
[12] Nevin Lianwen Zhang, Chen Fu, Teng Fei Liu, Bao-xin Chen, Kin Man Poon, Pei Xian Chen, Yunling Zhang. A data-driven method for syndrome type identification and classification in traditional Chinese medicine. Journal of Integrative Medicine, 2017, 15(2): 110-123.
[13] Nathalia Gomes Ribeiro de Moura, Ivan Cordovil, Arthurde Sá Ferreira. Traditional Chinese medicine wrist pulse-taking is associated with pulse waveform analysis and hemodynamics in hypertension. Journal of Integrative Medicine, 2016, 14(2): 100-113.
[14] Qing Ji, Yun-quan Luo, Wen-hai Wang, Xuan Liu, Qi Li, Shi-bing Su. Research advances in traditional Chinese medicine syndromes in cancer patients. Journal of Integrative Medicine, 2016, 14(1): 12-21.
[15] Reshma M.Ansari. Potential use of durian fruit (Durio zibenthinus Linn) as an adjunct to treat infertility in polycystic ovarian syndrome. Journal of Integrative Medicine, 2016, 14(1): 22-28.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] SUN Ming-yu, ZUO Jian, DUAN Ji-feng, HAN Jun, FAN Shi-ming, ZHANG Wei, ZHU Li-fang, YAO Ming-hui. Antitumor activities of kushen flavonoids in vivo and in vitro. Journal of Chinese Integrative Medicine, 2008, 6(1): 51-59
[3] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[8] Chan Chen, Yong-mei Meng , Peng Zhang, Juan Wang, Hui-hui Zhao, Shu-zhen Guo , Wei Wang. Diagnosis and treatment rule of traditional Chinese medicine for syndrome factors of chronic congestive heart failure: a study based on Shannon entropy method. Journal of Chinese Integrative Medicine, 2010, 8(11): 1080-1084
[9] Liang-ping Hu , Xiao-lei Bao, Xue Guan. Statistical inference and experimental design of univariate qualitative data of single-group design. Journal of Chinese Integrative Medicine, 2010, 8(11): 1085-1089
[10] Zhi-mei Wang, Bin Zhang. A study on translation of ellipses in Huangdi Neijing from perspective of hermeneutic theory. Journal of Chinese Integrative Medicine, 2010, 8(11): 1097-1100