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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (1): 74-79.doi: 10.3736/jcim20100114

• Original Experimental Research • Previous Articles     Next Articles

Effects of Xiaotan Sanjie Decoction on expressions of interleukin-8 and its receptors in gastric tumor xenografts and gastric tissue adjacent to the tumor in mice

 Da-wei Jua,Pin-kang Weia,Hui-ming Lina, Da-zhi Suna,Shan Yub,Li-juan Xiua   

  1. a Department of Traditional Chinese Medicine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
    b The 309th Hospital of Peopled Liberation Army, Beijing 100091, China
  • Received:2009-09-02 Accepted:2009-11-17 Online:2010-01-20 Published:2010-01-15
  • Contact: Pin-kang Wei E-mail:czzyk@smmu.edu.cn

Objective

To explore the mechanisms of Xiaotan Sanjie Decoction (XTSJD), a compound traditional Chinese herbal medicine, in inhibiting the tumor growth and preventing recurrence by testing the protein expressions of interleukin-8 (IL-8) and its receptors chemokine receptor 1 (CXCR1) and chemokine receptor 2 (CXCR2) in gastric tumor xenografts and gastric tissue adjacent to the tumor in mice.
Methods

Fifty Kunming mice were randomly divided into normal group, normal saline (NS) group, Heat-clearing and Detoxicating Decoction (HCDD) group, tegafur (FT-207) group and XTSJD group. Except for mice in the normal group, S180 tumor block was transplanted into the gastric walls of the mice, and the mice were administered with corresponding medicine for 3 weeks. Weight of tumor xenografts was measured and tumor inhibition rate was calculated. IL-8 protein expression was tested by enzyme-linked immunosorbent assay. Expressions of CXCR1 and CXCR2 were tested by immunohistochemical method.
Results

The protein expressions of IL-8 and its receptors in tumor xenografts and gastric tissue adjacent to the tumor were markedly higher than those in the gastric tissue in normal mice (P<0.01); compared with HCDD and FT-207, XTSJD could significantly decrease the IL-8 protein expression in tumor xenografts and gastric tissue adjacent to the tumor (P<0.05); compared with FT-207, XTSJD could significantly decrease the CXCR1 protein expression in tumor xenografts (P<0.01), and XTSJD could also significantly decrease the CXCR1 protein expression in gastric tissue adjacent to the tumor as compared with HCDD and FT-207 (P<0.01); compared with HCDD and FT-207, XTSJD could significantly decrease the CXCR2 protein expression in tumor xenografts (P<0.01), and there was no significant difference among the three drug-treated groups in CXCR2 protein expression in gastric tissue adjacent to the tumor (P>0.05).
Conclusion

XTSJD can decrease the protein expressions of IL-8 and its receptors in tumor xenografts and gastric tissue adjacent to the tumor. It may be one of the mechanisms of XTSJD in inhibiting the tumor growth and preventing recurrence.

Key words: Xiaotan Sanjie Decoction, Gastric tumor, Interleukin-8, Chemokine receptor 1, Chemokine receptor 2, Mice

Table 1

Weight of gastric tumor xenografts and inhibition rate in different groups"

Group n Tumor weight ($\bar{x}±s$, g) Inhibition ate (%)
NS 10 2.43±0.36
HCDD 10 1.38±0.28△△▲▲ 43.21
FT-207 10 1.17±0.32△△ 51.85
XTSJD 10 1.01±0.21△△ 58.02

Table 2

Expression of IL-8 protein in gastric tumor xenografts and gastric tissue adjacent to tumor in different groups ($\bar{x}±s$, ng/L)"

Group n IL-8 protein
Tumor tissue Gastric tissue
adjacent to tumor
Normal 10 7.92±4.35
NS 10 47.22±4.84** 21.99±5.14**
HCDD 10 40.62±4.61**△△▲▲ 15.87±4.26**△△▲
FT-207 10 38.53±3.79**△△▲▲ 15.90±4.14**△△▲
XTSJD 10 29.42±4.38**△△ 11.03±4.85△△

Table 3

Expression of CXCR1 protein in gastric tumor xenografts and gastric tissue adjacent to tumor in different groups"

Group n Field distribution of expression
of CXCR2 protein in tumor tissue
Field distribution of expression of CXCR2
protein in gastric tissue adjacent to tumor
+ ++ +++ P<0.01 + ++ +++ P<0.01
Normal 100 95 4 1 0
NS 100 22 17 20 41 vs normal group 63 6 13 18 vs normal group
HCDD 100 52 15 13 20 vs normal and NS groups 72 8 12 8 vs normal, NS and XTSJD groups
FT-207 100 48 11 18 23 vs normal, NS and XTSJD roups 71 7 12 10 vs normal, NS and XTSJD groups
XTSJD 100 60 15 15 10 vs normal and NS groups 86 11 3 0 vs normal and NS groups

"

Group n Field distribution of expression
of CXCR2 protein in tumor tissue
Field distribution of expression of CXCR2
protein in gastric tissue adjacent to tumor
+ ++ +++ P<0.01 + ++ +++ P<0.01
Normal 100 91 7 2 0
NS 100 11 18 24 47 vs normal group 55 11 19 15 vs normal group
HCDD 100 31 11 27 31 vs normal, NS and XTSJD groups 71 10 12 7 vs normal and NS groups
FT-207 100 32 13 36 19 vs normal, NS and XTSJD groups 70 14 10 6 vs normal and NS groups
XTSJD 100 59 21 13 7 vs normal and NS groups 78 6 10 6 vs normal and NS groups
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