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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (3): 269-274.doi: 10.3736/jcim20100312

• Original Experimental Research • Previous Articles     Next Articles

Effects of Xuesaitong Soft Capsule on hemodynamics and cardiocyte apoptosis of rats after myocardial infarction

Xue-ying Wang, Jie Wang,Ge Yang, Yong-mei Liu   

  1. Department of Cardiology, Guang’anmen Hospital,China Academy of Traditional Chinese Medicine,Beijing 100053,China
  • Received:2009-11-12 Accepted:2010-02-02 Online:2010-03-20 Published:2010-03-15
  • Contact: Jie Wang E-mail:wangjie0103@yahoo.com.cn

Objective

To observe the effects of Xuesaitong Soft Capsule, a compound traditional Chinese medicine, on hemodynamics and cardiocyte apoptosis of rats after myocardial infarction (MI) in different time and areas, and to explore its mechanism in inhibiting cardiac ventricle reconstitution and muscle remodeling.
Methods

Except rats in sham-operated group, MI was induced by ligaturing the left coronary artery main stem of rats' hearts. Then, rats were divided into untreated group and Xuesaitong group. Ejection fraction (EF) and fractional shortening (FS), hemodynamics, ratio of heart weight to body weight (HW/BW ratio), and pathological changes of cardiac tissue were analyzed to assess cardiac function and myocardial hypertrophy status 48 hours and 5 weeks after MI. Cardiocyte apoptosis in different areas was detected by fluorescently labeled TdT-mediated dUTP-biotin nick end labeling (TUNEL) and DNA ladder method.
Results

Comparing with the untreated group, EF and FS in the Xuesaitong group were significantly increased, and the apoptosis index was significantly decreased (P<0.01); 48 hours after MI, left ventricular systolic pressure and maximum rate of left ventricular pressure rise were obviously increased, while left ventricular end-diastolic pressure (LVEDP) and heart weight to body weight ratio were decreased as compared with the untreated group (P<0.01); LVEDP in the Xuesaitong group was decreased, while maximum rate of left ventricular pressure rise and fall were increased as compared with the untreated group 5 weeks after MI (P<0.01).
Conclusion

Xuesaitong can efficiently treat reconstitution and remodeling of cardiac ventricle of rats after myocardial infarction by improving the heart function and inhibiting the cardiocyte apoptosis.

Key words: Myocardial infarction, Compound (TCD), Ventricular function, Myocytes, Cardiac, Apoptosis, Rats

Figure 1

Pathological changes of cardiac tissues in rats after myocardial infarction in different time points observed by HE staining (Light microscopy, ×100)"

Table 1

Effects of PNS on HW/BW ratio of rats in different time points after myocardial infarction (x±s)"

Group n HW/BW ratio
Sham-operated
48 h 6 0.003 283±0.000 242
5 weeks 6 0.003 478±0.000 175
Untreated
48 h 17 0.004 469±0.000 304**
5 weeks 16 0.004 663±0.001 031**
Xuesaitong
48 h 15 0.003 960±0.000 273△△
5 weeks 17 0.004 592±0.000 743

Table 2

Effects of Xuesaitong on HR, ASP and ADP of rats in different time points after myocardial infarction (x±s)"

Group n HR (beats/min) ASP (mmHg) ADP (mmHg)
Sham-operated
48 h 6 427.17±22.25 162.77±24.86 125.44±18.13
5 weeks 6 384.67±80.46 144.45±11.35 114.86±8.80
Untreated
48 h 17 375.24±137.26 141.46±20.46 113.73±14.43
5 weeks 16 588.88±225.91* 151.32±28.30 122.10±23.52
Xuesaitong
48 h 15 396.73±153.87 145.47±16.29 113.59±11.08
5 weeks 17 479.29±210.62 153.60±27.15 120.61±19.75

Table 3

Effects of Xuesaitong on hemodynamic parameters of left ventricle of rats in different time points after myocardial infarction"

Group n LVSP (x±s, mmHg) LVEDP [M (Q25, Q75),
mmHg]
+dp/dt (x±s, mmHg/s) –dp/dt (x±s, mmHg/s)
Sham-operated
48 h 6 159.47±25.46 –14.89 (–7.44, –22.33) 7 481.7±994.2 –(10 020.0±0.0)
5 weeks 6 137.91±23.31 –15.43 (–7.72, -23.15) 6 906.7±1 351.8 –(8 646.7±2 503.2)
Untreated
48 h 17 126.25±30.17** 174.72 (87.36, 262.07)** 3 225.3±1 252.9** –(6 618.8±3 867.2)**
5 weeks 16 137.91±23.31 210.25 (105.12, 315.37)** 3 883.1±1 473.4** –(4 015.6±2 597.9)**
Xuesaitong
48 h 15 146.36±24.81 47.02 (23.51, 70.52)△△ 6 111.3±1 424.2△△ –(7 995.3±2 479.6)
5 weeks 17 147.64±20.59 84.08 (42.04, 126.12),△△ 5 777.6±1 683.6△△ –(6 003.5±2 644.2)△△

Table 4

Effects of Xuesaitong on heart function change of rats in different time points after myocardial infarction (x±s, %)"

Group n EF FS
Sham-operated
48 h 6 82.40±1.81 46.15±2.83
5 weeks 6 84.55±4.14 47.63±5.99
Untreated
48 h 17 52.06±5.76** 23.01±3.28**
5 weeks 16 53.98±5.83** 24.34±3.39**
Xuesaitong
48 h 15 65.64±6.57**△△ 31.60±4.84**△△
5 weeks 17 65.46±5.55**△△ 31.76±3.87**△△

Table 5

Effects of Xuesaitong on apoptosis index of rats in different time points after myocardial infarction (x±s, %)"

Group n Apoptosis index
Infarcted area Border area Non-infarcted area
Sham-operated
48 h 3 0 0 2.81±0.50
5 weeks 3 0 0 3.10±0.80
Untreated
48 h 6 63.79±3.27** 65.02±0.47** 57.93±3.25**
5 weeks 6 50.94±9.47** 55.74±12.37** 51.20±12.98**
Xuesaitong
48 h 6 47.66±5.12**△△ 43.61±16.90**△△ 29.28±10.19**△△
5 weeks 6 25.38±4.97**△△ 28.91±3.82** 15.35±7.26**△△

Figure 2

DNA ladder figure of rats 48 hours after myocardial infarctionA: Sham-operated group; B: Untreated group; C: Xuesaitong group; M: Marker."

Figure 3

DNA ladder figure of rats 5 weeks after myocardial infarctionA: Sham-operated group; B: Untreated group; C: Xuesaitong group; M: Marker."

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