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Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (5): 480-485.doi: 10.3736/jcim20100513

• Original Experimental Research • Previous Articles     Next Articles

Effects of electro-acupuncture on expression of obestatin in hypothalamus of rats with simple obesity

 Xian-juan Konga,b, Lei Gaoc, Hao Pengc, Xian Shia   

  1. a Department of Acupuncture and Moxibustion, General Hospital of Peopled Liberation Army, Beijing 100853, China
    b Health Section, August First Physical Culture and Sports Team of Peopled Liberation Army, Beijing 100091, China
    School of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China
  • Received:2010-03-31 Accepted:2010-04-21 Online:2010-05-20 Published:2010-09-15
  • Contact: Xian Shi E-mail:301sxian@sina.com

Objective

To investigate the effects of electro-acupuncture on obestatin in rat with simple obesity.
Methods

Obesity was induced in male SD rats with high-fat and -glucose diet for 10 weeks. Twenty-four rats with obesity were randomly divided into untreated group and electro-acupuncture group, with 12 rats in each group. Another 12 normal SD rats serving as controls were fed standard rat chow. Rats in the electro-acupuncture group were treated with electro-acupuncture on bilateral Zusanli (ST36), Tianshu (ST25) and Pishu (BL20) acupoints for fifteen days consecutively. Rats in the blank control group and untreated group were not interfered. Body weight was measured every 3 days during treatment. After treatment, obestatin in serum and hypothalamus was detected by radioimmunoassay or immunohistochemiscal method respectively. Visceral fat weight was also detected.
Results

Body weight and visceral fat weight of rats in the untreated group were significantly higher than those in the blank control group (P<0.01). Body weight and increase of body weight in the electro-acupuncture group were significantly lower than those in the untreated group (P<0.01). Visceral fat weight in the electro-acupuncture group was significantly lower than that in the untreated group (P<0.01), but there was no significant difference as compared with the blank control group. The obestatin expression in hypothalamus was higher in the untreated group than in the blank control group (P<0.05), while it was lower in the untreated group than in the electro-acupuncture group (P<0.01).
Conclusion

Electro-acupuncture has an effect against obesity in rats, which may be related to up-regulating obestatin expression in hypothalamus.

Key words: Simple obesity, Electro-acupuncture, Hypothalamus, Obestatin, Rats

Table 1

Food intake in different groups (x±s, g/d)"

Group n Food intake
Day 1 Day 3 Day 6 Day 9 Day 12 Day 15
Blank control 12 32. 37±3. 71 34. 58±2.00 35. 02±2. 82 36. 68±3. 70 35.38±2.72 34.05±2. 24
Untreated 12 26. 80±2. 74* * 31. 18±3. 55“ 34. 38±2. 81 37. 75±2. 89 40. 20±3. 76* * 40. 28±2. 97 * *
Electro-acupuncture 12 27. 97±4. 93* * 25. 98±1. 74* * AA 24. 86±1. 98* * AA 28. 92±4. 11* * A 30. 07±4. 13* * AA 26.55±2. 68*

Table 2

Body weight and visceral fat weight in different groups (x±s, g)"

Group Body weight Visceral £ftt weight
Before experiment After experiment Difference
Blank control 12 612,11士42,33 673,00 土 43,36“ SO, 88 土 U, 20 25,93土2,45
Untreated 12 764, 93土30,05 ?? 801,84 土 32,13 36,92 土 12,65 ?? 38,07±S,35〃
Electro-acupuncture 12 759,45 土 29,49 ?? 733,抖土23,38 -(25, 55土 19,30) 27,29士2,63^^

Table 3

Content of obestatin in serum and expression of obestatin in hypothalamus in rats (x±s)"

Group n Obestiim content in serum (ng/L) Obestain expreasion in hypothalamus
Blank control 12 1,34 土 0,10 0,33土0,03
Untreated 12 1, 54 土0,21 ? 0,36土0,03?
Electro-acupuncture 12 1,59土0,20〃 0,40±0,04maa

Figure 2

Expression of obestatin in hypothalamus in different groups observed by immunohistochemical method (Light microscopy, ×400) A: PBS control group; B: Blank control group; C: Untreated group; D: Electro-acupuncture group."

[1] Zhang JV, Ren PG, Avsian-Kretchmer O, Luo CW, Rauch R, Klein C, Hsueh AJ . Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin’s effects on food intake[J]. Science, 2005,310(5750):996-999
doi: 10.1126/science.1117255
[2] Xu HR, Zheng X, Guo ZF . Study on circulating levels of ghrelin and obestatin in human simple obesity[J]. Xin Zang Za Zhi, 2009,21(1):95-98
徐海蓉, 郑兴, 郭志福 . 单纯性肥胖症患者外周血ghrelin、obestatin的水平变化及其临床意义[J]. 心脏杂志, 2009,21(1):95-98
[3] Wang J . The influence of high lipid diet on serum and hypothalamic ghrelin, obestatin and the ratio of rats[J]. Ti Yu Ke Xue, 2007,27(11):61-65
汪军 . 高脂饮食对大鼠血浆和下丘脑Ghrelin、Obestatin及其比值的影响[J]. 体育科学, 2007,27(11):61-65
[4] Li WH, Wang JD, Gu LM, Wang YZ . Treatment of simple obesity with electro-acupuncture and auricular acupoint pressing: a report of 177 cases[J]. J Chin Integr Med, 2004,2(6):449-450
李伟红, 汪金娣, 谷丽敏, 王勇姿 . 电针配合耳穴贴压治疗单纯性肥胖病177例临床观察[J]. 中西医结合学报, 2004,2(6):449-450
[5] Xu B, Yuan JH, Liu ZC, Chen M, Wang XJ . Effect of acupuncture on plasma peptide YY in the patient of simple obesity[J]. Zhongguo Zhen Jiu, 2005,25(12):837-840
徐斌, 袁锦虹, 刘志诚, 陈梅, 王欣君 . 针刺影响单纯肥胖病患者血浆酪酪肽水平观察[J]. 中国针灸, 2005,25(12):837-840
[6] Huang QF, Zhao SX, Huang Y . Statistical analysis of the points for acupuncture-moxibustion treatment of obesity[J]. J Chin Integr Med, 2007,5(6):695-696
黄琴峰, 赵善祥, 黄颖 . 针灸治疗肥胖病的用穴统计分析[J]. 中西医结合学报, 2007,5(6):695-696
[7] Sun Z, Zhang ZC, Liu ZC . Experiment study of diet-induced obesity animal model[J]. Zhongguo Yao Li Xue Tong Bao, 2002,18(4):466-467
孙志, 张中成, 刘志诚 . 营养性肥胖动物模型的实验研究[J]. 中国药理学通报, 2002,18(4):466-467
[8] Yu SG, Guo Y. Experimental acupuncture science[M]. Shanghai: Shanghai Scientific and Technical Publishers, 2009: 150-151
余曙光, 郭义 . 实验针灸学[M]. 上海: 上海科学技术出版社, 2009: 150-151
[9] Shanghai Medical College of Fudan University, Editorial Committee of Practical Internal Medicine, Chen HZ . Practical internal medicine(12th ed)[M]. Beijing: People’s Medical Publishing House, 2005,( 12th ed):914-917
复旦大学上海医学院, 《实用内科学》编委会, 陈灏珠 . 实用内科学(第12版)[M]. 北京: 人民卫生出版社, 2005,( 第12版):914-917
[10] Qader SS, Håkanson R, Rehfeld JF, Lundquist I, Salehi A . Proghrelin-derived peptides influence the secretion of insulin, glucagon, pancreatic polypeptide and somatostatin: a study on isolated islets from mouse and rat pancreas[J]. Regul Pept, 2008,146(1-3):230-237
doi: 10.1016/j.regpep.2007.09.017
[11] Tang SQ, Jiang QY, Zhang YL, Zhu XT, Shu G, Gao P, Feng DY, Wang XQ, Dong XY . Obestatin: its physicochemical characteristics and physiological functions[J]. Peptides, 2008,29(4):639-645
doi: 10.1016/j.peptides.2008.01.012
[12] Zhang Z, Zou DJ, Chen Y, Wang M, Wu J, Guo ZF . Obestatin inhibits proliferation and differentiation of 3T3-L1 preadipocytes[J]. Di Er Jun Yi Da Xue Xue Bao, 2007,28(9):929-932
张征, 邹大进, 陈月, 王淼, 吴捷, 郭志福 . Obestatin抑制3T3-L1前脂肪细胞的增殖与分化[J]. 第二军医大学学报, 2007,28(9):929-932
[13] Li ZY, Tang CS . Research progress in obestatin[J]. Sheng Li Ke Xue Jin Zhan, 2007,38(2):147-149
李战永, 唐朝枢 . 肥胖抑制素的研究进展[J]. 生理科学进展, 2007,38(2):147-149
[14] Nogueiras R , Tschöp M .Biomedicine. Separation of conjoined hormones yields appetite rivals[J]. Science, 2005,310(5750):985-986
doi: 10.1126/science.1121214
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