Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (6): 568-574.doi: 10.3736/jcim20100610

• Original Experimental Research • Previous Articles     Next Articles

Effects of Bawei Xilei San on mice with oxazolone-induced colitis and the mechanisms

Yan Pan, Qin Ouyang   

  1. Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2010-01-20 Accepted:2010-03-31 Online:2010-06-20 Published:2010-06-15
  • Contact: Qin Ouyang E-mail:qin.ouyang@163.com

Objective

To investigate the therapeutic effects of Bawei Xilei San (BWXLS), a compound traditional Chinese herbal medicine, on mice with oxazolone-induced colitis and to explore the mechanisms.
Methods

Thirty-two BALB/c mice were randomly divided into 4 groups (8 for each): normal control group, untreated group, hydrocortisone group and BWXLS group. Except for the mice in the normal control group, all mice were intrarectally administered with 3.0% oxazolone to induce colitis. Then the mice in the normal control group and untreated group were administered with 0.9% carboxymethyl cellulose sodium solution. Mice in the BWXLS group were intrarectally administered with 0.2 mg/g BWXLS and hydrocortisone group with 0.02 mg/g respectively for 5 days. The body weight and stool consistency and occult or gross blood were recorded to calculate the disease activity index (DAI). The mice were sacrificed at the 6th day. The macroscopic and histological changes of the colon were evaluated. The expressions of Toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB), and epithelial tight junction protein occludin were assessed by immunohistochemical method. The level of tumor necrosis factor-α (TNF-α) in colonic mucosa was evaluated by enzyme-linked immunosorbent assay.
Results

The DAI, and macroscopic and histological changes in the BWXLS group were improved as compared with those in the untreated group (P<0.05) but were similar to those in the hydrocortisone group. The expression of occludin was significantly increased (P<0.05) while the expressions of TLR4, NF-κB and TNF-α were significantly decreased in the BWXLS group as compared with the untreated group, and were similar to those in the hydrocortisone group (P>0.05).
Conclusion

Up-regulating the expression of occludin and down-regulating the expressions of TLR4 and NF-κB, and hence inhibiting TNF-α expression and improving the mucosa barrier function may be part of the mechanisms of BWXLS in treating oxazolone-induced colitis in mice.

Key words: Compound (TCD), Oxazolone, Colitis, Mice

"

Figure 1

Disease activity index of mice in different groups"

"

"

Figure 2

Histological changes of colon in mice in different groups observed by HE staining (Light microscopy, ×400)A: Normal group; B: Untreated group; C: Hydrocortisone group; D: Bawei Xilei San group."

Figure 3

Expression of occludin in colonic mucosa of mice in different groups observed by immunohistochemical method (Light microscopy, ×400)A: Normal group; B: Untreated group; C: Hydrocortisone group; D: Bawei Xilei San group."

Figure 4

Expression of TLR4 in colonic mucosa of mice in different groups observed by immunohistochemical method (Light microscopy, ×400)A: Normal group; B: Untreated group; C: Hydrocortisone group; D: Bawei Xilei San group."

Figure 5

Expression of NF-κB in colonic mucosa of mice in different groups observed by immunohistochemical method (Light microscopy, ×400)A: Normal group; B: Untreated group; C: Hydrocortisone group; D: Bawei Xilei San group."

[1] Baumgart DC, Carding SR . Inflammatory bowel disease: cause and immunobiology[J]. Lancet, 2007,369(9573):1627-1640
doi: 10.1016/S0140-6736(07)60750-8
[2] Jiang XL, Cui HF . An analysis of 10 218 ulcerative colitis cases in China[J]. World J Gastroenterol, 2002,8(1):158-161
doi: 10.3748/wjg.v8.i1.158
[3] Fukunaga K, Hida N, Ohnishi K, Ohda Y, Yoshida K, Kusaka T, Jinno Y, Nagase K, Nakamura S, Kadobayashi M, Miva H, Matsumoto TA . A suppository Chinese medicine(Xilei-san) for refractory ulcerative proctitis:a pilot clinical trial[J]. Digestion, 2007,75(2-3):146-147
doi: 10.1159/000106755
[4] Heller F, Fuss IJ, Nieuwenhuis EE, Blumberg RS, Strober W . Oxazolone colitis, a Th2 colitis model resembling ulcerative colitis, is mediated by IL-13-producing NK-T cells[J]. Immunity, 2002,17(5):629-638
doi: 10.1016/S1074-7613(02)00453-3
[5] Chen Q. Technology of research on pharmacology of traditional Chinese medicine(2nd ed)[M]. Beijing: People's Medical Publishing House, 2006: 1170
陈奇 . 中药药理研究方法学(第2版)[M]. 北京: 人民卫生出版社, 2006: 1170
[6] Kullmann F, Messmann H, Alt M, Gross V, Bocker T , Schölmerich J, Rüschoff J.Clinical and histopathological features of dextran sulfate sodium induced acute and chronic colitis associated with dysplasia in rats[J]. Int J Colorectal Dis, 2001,16(4):238-246
doi: 10.1007/s003840100311
[7] Ekstrm GM . Oxazolone-induced colitis in rats: effects of budesonide, cyclosporin A, and 5-aminosalicylic acid[J]. Scand J Gastroenterol, 1998,33(2):174-179
doi: 10.1080/00365529850166914
[8] Han Y, Murata Y, Itoh J, Munakata A . Effect of long-term nicotine on oxazolone-induced murine colitis model[J]. Zhonghua Xiao Hua Za Zhi, 2001,21(8):473-476
韩英, 村田有志, 伊东重豪, 栋方昭博 . 长期应用尼古丁对恶唑酮诱导的实验性小鼠肠炎模型的影响及其机制探讨[J]. 中华消化杂志, 2001,21(8):473-476
[9] Liu DY, Zhao HM, Lu AP . Compare of Bawei Xilei Powder with Xilei Powder[J]. Zhongguo Zhong Yi Ji Chu Yi Xue Za Zhi, 2004,10(6):11-12
刘端勇, 赵海梅, 吕爱平 . 八味锡类散与锡类散之鉴赏[J]. 中国中医基础医学杂志, 2004,10(6):11-12
[10] Regueiro M, Loftus EV Jr, Steinhart AH, Cohen RD . Medical management of left-sided ulcerative colitis and ulcerative proctitis: critical evaluation of therapeutic trials[J]. Inflamm Bowel Dis, 2006,12(10):979-994
doi: 10.1097/01.mib.0000231495.92013.5e
[11] Liu DY, Zhao HM, Zhao N, Lu C, Lu AP . Effect of Bawei Xilei powder on CD3, CD4, CD8 T-lymphocytes of rats with ulcerative colitis[J]. Zhongguo Zhong Yao Za Zhi, 2008,33(11):1301-1304
刘端勇, 赵海梅, 赵宁, 吕诚, 吕爱平 . 八味锡类散对大鼠溃疡性结肠炎CD3, CD4, CD8 T淋巴细胞的影响[J]. 中国中药杂志, 2008,33(11):1301-1304
[12] Sethi G, Ahn KS, Sandur SK, Lin X, Chaturvedi MM, Aggarwal BB . Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway[J]. J Biol Chem, 2006,281(33):23425-23435
doi: 10.1074/jbc.M602627200
[13] Lopez E, Le Faou A, Borzeix S, Berland S . Stimulation of rat cutaneous fibroblasts and their synthetic activity by implants of powdered nacre(mother of pearl)[J]. Tissue Cell, 2000,32(1):95-101
doi: 10.1054/tice.1999.0091
[14] Nusrat A, Chen JA, Foley CS, Liang TW, Tom J, Cromwell M, Quan C, Mrsny RJ . The coiled-coil domain of occludin can act to organize structural and functional elements of the epithelial tight junction[J]. J Biol Chem, 2000,275(38):29816-29822
doi: 10.1074/jbc.M002450200
[15] Kucharzik T, Walsh SV, Chen J, Parkos CA, Nusrat A . Neutrophil transmigration in inflammatory bowel disease is associated with differential expression of epithelial intercellular junction proteins[J]. Am J Pathol, 2001,159(6):2001-2009
doi: 10.1016/S0002-9440(10)63051-9
[16] Li RL, Chi L, Guo WF, Li QX, Xu SF, Chen WW . Tight junction protein occludin and claudin-1 expression in ulcerative colitis rats[J]. Guangzhou Zhong Yi Yao Da Xue Xue Bao, 2008,25(1):83-87, 90
李茹柳, 迟莉, 郭文峰, 李秋霞, 徐颂芬, 陈蔚文 . 紧密连接蛋白occludin和claudin-1在大鼠溃疡性结肠炎的表达[J]. 广州中医药大学学报, 2008,25(1):83-87, 90
[17] Xu N, Ouyang Q . Toll-like receptors, NF-κB and inflammatory bowel disease[J]. Guo Wai Yi Xue Xiao Hua Xi Ji Bing Fen Ce, 2003,23(5):264-267
徐宁, 欧阳钦 . Toll样受体, NF-κB与炎症性肠病[J]. 国外医学: 消化系疾病分册, 2003,23(5):264-267
[18] Abreu MT, Arnold ET, Thomas LS, Gonsky R, Zhou Y, Hu B, Arditi M . TLR4 and MD-2 expression is regulated by immune-mediated signals in human intestinal epithelial cells[J]. J Biol Chem, 2002,277(23):20431-20437
doi: 10.1074/jbc.M110333200
[1] Victor Abou Nehmi Filho, Gilson Masahiro Murata, Ruan Carlos Macêdo de Moraes, Gabriely Cristina Alves Lima, Danielle Araujo de Miranda, Katrin Radloff, Raquel Galvão Figuerêdo Costa, Joyce de Cassia Rosa de Jesus, Jéssica Alves De Freitas, Nayara Izabel Viana, Ruan Pimenta, Katia Ramos Moreira Leite, José Pinhata Otoch, Ana Flávia Marçal Pessoa. A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice. Journal of Integrative Medicine, 2021, 19(5): 439-450.
[2] Hao Gou, Ruo-chen Huang, Yong-hua Su, Wei Li. Design of dual targeting immunomicelles loaded with bufalin and study of their anti-tumor effect on liver cancer. Journal of Integrative Medicine, 2021, 19(5): 408-417.
[3] Li-qing Zhu, Li Zhang, Jia Zhang, Guo-lin Chang, Gang Liu, Dan-dan Yu, Xiao-min Yu, Mi-sheng Zhao, Bin Ye. Evodiamine inhibits high-fat diet-induced colitis-associated cancer in mice through regulating the gut microbiota. Journal of Integrative Medicine, 2021, 19(1): 56-65.
[4] Yi Ran Hu, San Li Xing, Chuan Chen, Ding Zhu Shen, Jiu Lin Chen. Tiaoxin Recipe, a Chinese herbal formula, inhibits microRNA-34a expression in the APPswe/PS1ΔE9 mouse model of Alzheimer's disease. Journal of Integrative Medicine, 2019, 17(6): 404-409.
[5] Bhesh Raj Sharma, Dong Wook Kim, Dong Young Rhyu. Korean Chungtaejeon tea extract attenuates weight gain in C57BL/6J-Lep ob/ob mice and regulates adipogenesis and lipolysis in 3T3-L1 adipocytes. Journal of Integrative Medicine, 2017, 15(1): 56-63.
[6] Shi-li Jiang, Xu-dong Hu, Ping Liu. Immunomodulation and liver protection of Yinchenhao decoction against concanavalin A-induced chronic liver injury in mice. Journal of Integrative Medicine, 2015, 13(4): 262-268.
[7] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[8] Md. Asadujjaman, Ahmed Ullah Mishuk, Md. Aslam Hossain, Utpal Kumar Karmakar. Medicinal potential of Passiflora foetida L. plant extracts: Biological and pharmacological activities. Journal of Integrative Medicine, 2014, 12(2): 121-126.
[9] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
[10] Md. Khirul Islam, Imran Mahmud, Sanjib Saha, Asit Baron Sarker, Himangsu Mondal, A.S.M. Monjur-Al-Hossain, Md. Anisuzzman. Preliminary pharmacological evaluation of Alocasia indica Schott tuber. Journal of Chinese Integrative Medicine, 2013, 11(5): 343-351.
[11] Marjan Nassiri-Asl, Taghi Naserpour Farivar, Esmail Abbasi, Hamid Reza Sadeghnia, Mehdi Sheikhi, Mina Lotfizadeh, Parisa Bazahang. Effects of rutin on oxidative stress in mice with kainic acid-induced seizure. Journal of Chinese Integrative Medicine, 2013, 11(5): 337-342.
[12] Sanjib Saha, Faroque Hossain, Md. Anisuzzman, Md. Khirul Islam. Pharmacological evaluation of Musa seminifera Lour. Fruit. Journal of Chinese Integrative Medicine, 2013, 11(4): 253-261.
[13] Sunil A. Nirmal, Jayashri M. Ingale, Shashikant R. Pattan, Sanjay B. Bhawar. Amaranthus roxburghianus root extract in combination with piperine as a potential treatment of ulcerative colitis in mice. Journal of Chinese Integrative Medicine, 2013, 11(3): 206-212.
[14] Zi-hui Li, Jian Wang, Rong-lin Cai, You-wen Wang, Jian-peng Hu. Establishment and evaluation of a rat model of ulcerative colitis with syndrome of dampness stagnancy due to spleen deficiency. Journal of Chinese Integrative Medicine, 2012, 10(8): 918-924.
[15] Dong-mei Zhang, Li-xia Lou, Ai-ming Wu, Xi-ying Lü, Zhao-juan Hu, Yan-hong Zhang, Hong-fang Liu. Effects of Astragalus membranaceus and Potentilla discolor mixture on insulin resistance and its related mRNA expressions in KKAy mice with type 2 diabetes. Journal of Chinese Integrative Medicine, 2012, 10(7): 821-826.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[2] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[3] Xi Lin, Jian-ping Liu. Herbal medicines for viral myocarditis. Journal of Chinese Integrative Medicine, 2008, 6(1): 76
[4] Xi Lin, Jian-ping Liu. Tai chi for treating rheumatoid arthritis. Journal of Chinese Integrative Medicine, 2008, 6(1): 82
[5] Liang-ping Hu, Hui Gao. Discrimination of errors in statistical analysis of medical papers published in the first issue of 2006 in Journal of Chinese Integrative Medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 98-106
[6] Yan-bo Zhu , Qi Wang, Cheng-yu Wu, Guo-ming Pang, Jian-xiong Zhao, Shi-lin Shen, Zhong-yuan Xia , Xue Yan . Logistic regression analysis on relationships between traditional Chinese medicine constitutional types and overweight or obesity. Journal of Chinese Integrative Medicine, 2010, 8(11): 1023-1035
[7] Wei Xu, Meng Shi, Jian-gang Liu, Cheng-long Wang . Collagen protein expressions in ischemic myocardium of rats with acute myocardial infarction and effects of qi-tonifying, yin-tonifying and blood-activating herbs and detoxifying and blood-activating herbs. Journal of Chinese Integrative Medicine, 2010, 8(11): 1041-1047
[8] Tao Wang , Feng Qin. Effects of Chinese herbal medicine Xiaoyao Powder on monoamine neurotransmitters in hippocampus of rats with postpartum depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1075-1079
[9] Ying Xu , Chang-chun Zeng , Xiu-yu Cai , Rong-ping Guo , Guang Nie , Ying Jin. Chromaticity and optical spectrum colorimetry of the tongue color in different syndromes of primary hepatic carcinoma. Journal of Chinese Integrative Medicine, 2012, 10(11): 1263-1271
[10] Xiang-ying Mao , Qin Bian , Zi-yin Shen. Analysis of the osteogenetic effects exerted on mesenchymal stem cell strain C3H10T1/2 by icariin via MAPK signaling pathway in vitro. Journal of Chinese Integrative Medicine, 2012, 10(11): 1272-1278