促性腺激素释放激素拟似剂对青春期大鼠生长轴与性腺轴相关基因表达的影响

doi:10.3736/jcim20030115

Abstract

Objective

To elucidate the effect and effective mechanism of gonadotropin-releasing hormone analog (GnRHa) on growth axis and gonadal axis in puberty rats.

Methods

After injecting GnRHa on puberty rats, hypothalamus, pituitary, ovary and epiphyseal cartilage of legs were taken, and related gene expression was detected by fluorescence quantitative PCR (FQ-PCR).

Results

GnRHa could reduce the gene expression of gonadotropin-releasing hormone (GnRH) in hypothalamus and its receptor (GnRHR) in pituitary, increase the gene expression of growth hormone releasing inhibiting hormone (SRIH), and not affect the gene expression of growth hormone releasing hormone (GHRH) in hypothalamus. And GnRHa could reduce the gene expression of growth hormone (GH) in pituitary, estrogen receptor (ER) in ovary and insulin-like growth factors-I (IGF-1) in epiphyseal cartilage of legs.

Conclusion

Besides restraining the gene expression of GnRHR in pituitary and leading to descent regulation of receptor, GnRHa can reduce the gene expression of GnRH in hypothalamus and down-regulate gonadal hormone level. At last, GnRHa can reduce the maturity degree and pace of the second sexual characteristics. By means of increasing the gene expression of SRIH in hypothalamus, GnRHa can reduce the gene expression of GH in pituitary and IGF-1 in epiphyseal cartilage of legs. It could be one of the mechanisms of GnRHa reducing epiphyseal maturity for the patients with idiopathic central precocious puberty.

Key words: GnRHa, growth axis, gonadal axis, gene expression, FQ-PCR

CLC Number:

R34-33

Jia-min Wu, Jian Yu, Yi Yang. Gene expression related to growth axis and gonadal axis in puberty rats. Journal of Chinese Integrative Medicine, 2003, 1(1): 35-38.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20030115

http://www.jcimjournal.com/EN/Y2003/V1/I1/35

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项目	取材时间	空白对照组	西药注射组
GnRH	鼠龄12周	6.79±0.38	5.34±0.34*
	鼠龄15周	6.14±0.34	6.55±0.31
GnRHR	鼠龄12周	7.85±0.37	6.43±0.34*
	鼠龄15周	7.73±0.35	7.57±0.37
ER	鼠龄12周	6.83±0.62	4.84±0.23*
	鼠龄15周	5.79±0.26	5.73±0.21

项目	取材时间	空白对照组	西药注射组
GHRH	鼠龄12周	5.99±0.64	5.96±0.77
	鼠龄15周	5.50±0.64	5.65±0.38
SRIH	鼠龄12周	5.45±0.16	6.54±0.36*
	鼠龄15周	6.07±0.40	6.15±0.42

项目	取材时间	空白对照组	西药注射组
GH	鼠龄12周	8.83±0.44	7.86±0.10*
	鼠龄15周	8.18±0.67	7.70±0.33
IGF-1	鼠龄12周	6.10±0.55	4.29±0.23*
	鼠龄15周	5.66±0.35	5.43±0.42

Gene expression related to growth axis and gonadal axis in puberty rats

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