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Journal of Chinese Integrative Medicine ›› 2004, Vol. 2 ›› Issue (6): 450-452.doi: 10.3736/jcim20040615

• Original Experimental Research • Previous Articles     Next Articles

A study on anti-inflammatory and analgesic effects of alkaloids of Toddalia asiatica

Xiao-yan Hao1(), Lin Peng1, Lang Ye2, Neng-hui Huang2, Yue-mao Shen3   

  1. 1. Department of Analytical Chemistry, Guiyang Medical College, Guiyang, Guizhou Province 550004, China
    2. Department of Pharmacology, Guiyang Medical College, Guiyang, Guizhou Province 550004, China
    3. The Key Laboratory of Chemistry for Natural Products of Chinese Academy of Sciences, Guiyang, Guizhou Province 550002, China
  • Received:2003-06-20 Online:2004-11-20 Published:2018-10-20

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Objective: To study the pharmacological activities and toxicity of the crude alkaloids of Toddalia asiatica and to provide pharmacological data for the further development of this herbal medicine.Methods: We observed the anti-inflammatory effects of the crude alkaloids of Toddalia asiatica, using xylol and agra to induce the turgidness and sodium carboxymethyl cellulose (CMC-Na) to induce leucocyte strolling in the rats. The analgesic effects were observed by body-distortion methods. The effects of alkaloids of Toddalia asiatica on hepatic function were observed by testing the contents of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and calculating the liver index. The LD50 and 95% creditability were calculated with developed Karber Method.Results: The administration of alkaloids of Toddalia asiatica had the function of inhibiting the auricle swelling caused by xylol and joint swelling caused by agar and leucocyte migration caused by CMC-Na, decreasing the body-distortion of the rats. After two-week administration, the contents of ALT and AST showed that there was no obvious difference between administered group and control group. The LD50 of the crude alkaloids of Toddalia asiatica was 1.622 g/kg and the 95% creditability was 1.29-2.03 g/kg.Conclusion: Toddalia asiatica has anti-inflammatory and analgesic effects, and there is no injury to the liver after long-term administration in rats.

Key words: Toddalia asiatica, anti-inflammation, analgesia, lethal dose 50

CLC Number: 

  • R285.5

Tab 1

Effects of alkaloids of Toddalia asiatica on auricle and joint swelling of rats ($\bar{x}$±s, mg )"

Group n Weight difference of two ears Weight difference of two feet
Normal saline control 10 18.8±9.2 90.0±25.0
Solvent control 10 15.6±8.9 100.4±18.1
Low dose of alkaloids (0.08 g/kg) 10 16.6±7.1 86.6±28.2
Medium dose of alkaloids (0.10 g/kg) 10 7.7±4.7* 61.0±40.1*
High dose of alkaloids (0.16 g/kg) 10 13.4±6.8 93.9±24.0*
Aspirin (0.20 g/kg) 10 7.5±4.2* 77.0±20.3*

Tab 2

Effect of alkaloids of Toddalia asiatica on leucocyte migration caused by CMC-Na ($\bar{x}$±s )"

Group n Leucocytes (×105/L)
Normal saline control 10 95±20
Model 10 149±57
Solvent control 10 146±44
Low dose of alkaloids (0.08 g/kg) 10 128±26
Medium dose of alkaloids (0.10 g/kg) 10 111±62
High dose of alkaloids (0.16 g/kg) 10 108±37△
Aspirin (0.20 g/kg) 10 100±24△

Tab 3

Analgesic effect of alkaloids of Toddalia asiatica on rats (Body-distortion Procedure) ($\bar{x}$±s )"

Group n Body-distortion
(Distortions/10 min)
Normal saline control 10 17.4±9.4
Solvent control 10 16.7±8.2
Low dose of alkaloids (0.08 g/kg) 10 13.4±10.4
Medium dose of alkaloids (0.10 g/kg) 10 11.7±4.7*
High dose of alkaloids (0.16 g/kg) 10 9.3±7.4*
Aspirin (0.20 g/kg) 10 6.0±5.0*

Tab 4

Effect of alkaloids of Toddalia asiatica on hepatic function of rats ($\bar{x}$±s )"

Group n ALT （U/L） AST （U/L） Liver index （mg/g）
Normal saline control 10 66.80±5.72 67.41±6.25 55.8±8.7
Solvent control 10 69.12±9.43 68.97±10.23 53.9±2.8
Low dose of alkaloids (0.08 g/kg) 10 65.15±4.21 70.00±8.85 55.3±9.0
High dose of alkaloids (0.16 g/kg) 10 70.94±6.76 72.59±9.54 61.6±7.7
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