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Journal of Chinese Integrative Medicine ›› 2005, Vol. 3 ›› Issue (4): 290-293.doi: 10.3736/jcim20050413

• Original Experimental Research • Previous Articles     Next Articles

Effects of Huatan Xiezhuo Recipe on insulin resistance and leptin in rats with fatty liver

Fang Ye1,Wen-xia Zhao2(),Ming-san Miao2,Jian-guo Li2,Yong-yan Zhang2   

  1. 1. First Clinical College, Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu Province 210029, China
    2. Institute of Liver Diseases, Henan College of Traditional Chinese Medicine, Zhengzhou, Henan Province 450008, China
  • Received:2004-09-20 Online:2005-07-20 Published:2005-07-20
  • Contact: Wen-xia Zhao E-mail:zhao8536@hotmail.com

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Objective

To observe the effects of Huatan Xiezhuo Recipe (HTXZR) on fatty liver of rats.

Methods

Forty-eight SD rats were randomly divided into five groups: normal control group, untreated group, Dongbao Gantai Tablet (DBGTT)-treated group, low-dose HTXZR-treated group and high-dose HTXZR-treated group. Fatty liver was induced in the rats by hyperlipid diet and intraperitoneal injection of tetracycline. The pathological changes of liver tissues in rats were observed, and the liver function, serum leptin, insulin resistance index, triglyceride (TG) and free fatty acid (FFA) in the liver were detected.

Results

The levels of serum leptin, insulin resistance index, FFA and TG in rats of the three treated groups were lower than those of the untreated group (P<0.01), and such effects in the HTXZR-treated groups were more significant than those in the DBGTT-treated group (P<0.05).

Conclusion

The main action mechanisms of HTXZR in treating fatty liver are probably to promote the lipid metabolism, decrease the leptin and insulin resistance.

Key words: Huatan Xiezhuo Recipe, Fatty liver, Free fatty acid, Insulin resistance, Leptin resistance

CLC Number: 

  • R589.2

Tab 1

Changes of liver weight and liver index of the rats ($\bar{x}$±s)"

Group n Body weight (g) Liver index (%)
Normal control group 8 275.8±7.9 3.02±0.17
Untreated group 9 333.6±15.2▲▲ 4.10±0.24▲▲
DBGTT-treated group 10 311.0±10.7*▲▲ 3.80±0.20*▲▲
Low-dose HTXZR-treated group 10 300.6±12.8*▲▲ 3.55±0.17*△▲▲
High-dose HTXZR-treated group 10 283.5±11.1*▲▲ 3.68±0.16*▲▲

Tab 2

Changes of serum ALT, AST, leptin and insulin resistance index of the rats ($\bar{x}$±s)"

Group n ALT (U/L) AST (U/L) Leptin (μg/L) Insulin resistance index
Normal control group 8 48.88±4.76 149.75±5.37 0.29±0.06 9.94±0.81
Untreated group 9 425.87±72.93 292.12±30.99 1.08±0.37 14.69±1.93
DBGTT-treated group 10 212.13±19.17* 249.63±27.60 0.86±0.20 12.44±1.62*
Low-dose HTXZR-treated group 10 167.11±24.44*△△ 188.44±25.52*△△ 0.69±0.19* 14.10±2.63
High-dose HTXZR-treated group 10 150.88±19.31**△△ 204.50±15.90**△△ 0.62±0.16*△ 12.84±1.79*

Tab 3

Changes of liver tissue FFA, TG and serum TG of the rats ($\bar{x}$±s)"

Group n Liver homogenate FFA
(μmol/g)		 Liver omogenate TG
(mmol/L)		 Serum TG
(mmol/L)
Normal control group 8 123.71±16.37 0.56±0.05 0.85±0.19
Untreated group 9 560.50±81.67 7.38±0.55 1.49±0.45
DBGTT-treated group 10 382.73±36.71** 5.19±0.52** 1.47±0.44
Low-dose HTXZR-treated group 10 324.46±34.51**△△ 4.68±0.43**△ 1.26±0.34
High-dose HTXZR-treated group 10 280.51±14.78**△△ 3.77±0.31**△△ 1.43±0.31
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