山柰挥发油对裸鼠原位移植人胃癌生长和细胞周期的影响

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Effects of Rhizoma kaempferiae volatile oil on tumor growth and cell cycle of MKN-45 human gastric cancer cells orthotopically transplanted in nude mice

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doi:10.3736/jcim20060413

Objective

To evaluate the effects of Rhizoma kaempferiae volatile oil on tumor growth and cell cycle of MKN-45 human gastric cancer cells orthotopically transplanted in nude mice.

Methods

One hundred and five nude mice orthotopically transplanted with MKN-45 human gastric cancer cells were randomly divided into seven groups: untreated group, normal saline-treated group, dissolvant-treated group, cyclophosphamide (CTX)-treated group and high-, medium-, and low-dose Rhizoma kaempferiae volatile oil-treated groups. Corresponding interventions were implemented in each group except the untreated group. The antitumor effects in vivo were evaluated. Cell cycle distribution and apoptosis of MKN-45 human gastric cancer cells were determined by using flow cytometry (FCM). The ultrastructure of MKN-45 gastric cancer cells was observed by a transmission electron microscope.

Results

In the high-, medium-, and low-dose Rhizoma kaempferiae volatile oil-treated groups, the growth inhibition rates of gastric cancer were 57.2%, 28.0% and 5.0% respectively, and the gastric cancer cells were arrested at G0/G1 phase. This antitumor effect was dose-dependent. The apoptotic cells occurred more frequently in the high-dose Rhizoma kaempferiae volatile oil-treated group and the CTX-treated group than those in the medium- and low-dose Rhizoma kaempferiae volatile oil-treated groups.

Conclusion

The Rhizoma kaempferiae volatile oil is an effective composition for growth inhibition of gastric cancer, and its mechanism may be related to regulating the cell cycle and inducing apoptosis.

Key words: Rhizoma kaempferiae, Volatile oil, Stomach neoplasms, Orthotopic transplantation, Apoptosis, Cell cycle, Nude mice

CLC Number:

R735.2

Yan Xiao, Pin-kang Wei, Jun Li, Jun Shi, Zhi-hong Yu, Hui-ming Lin. Effects of Rhizoma kaempferiae volatile oil on tumor growth and cell cycle of MKN-45 human gastric cancer cells orthotopically transplanted in nude mice. Journal of Chinese Integrative Medicine, 2006, 4(4): 384-387.

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URL: http://www.jcimjournal.com/EN/10.3736/jcim20060413

http://www.jcimjournal.com/EN/Y2006/V4/I4/384

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Group	n	Tumor weight (ヌ±S, g)	Growth inhibition rate (%)
Rhizoma kaempferiae volatile oil-treated
High-dose	15	1.194±0.617**	57.2
Medium-dose	15	2.009±1.927	28.0
Low-dose	15	2.636±1.532	5.0
Dissolvant-treated	15	2.898±2.180	–
CTX-treated	15	0.587±0.146**	78.9
Normal saline-treated	15	2.774±0.662	0.7
Untreated	15	2.794±0.799	–

Group	n	G0/G1	S	G2/M	Apoptotic rate
Rhizoma kaempferiae volatile oil-treated
High-dose	15	64.55±12.58*	23.15±8.89*	12.23±3.86	27.10±14.03*
Medium-dose	15	60.36±12.37	24.04±9.24	15.41±8.33	25.10±11.97*
Low-dose	15	57.45±10.08	27.99±11.99	14.55±5.44	19.73±9.26
Dissolvant-treated	15	54.76±12.06	30.61±9.97	14.68±7.83	17.07±8.16
CTX-treated	14	56.73±11.69	28.91±8.08	14.52±4.03	25.86±8.37*
Normal saline-treated	15	52.21±10.24	31.87±6.83	15.91±3.41	16.82±9.84
Untreated	15	53.58±11.45	31.12±7.74	15.30±3.75	17.33±7.63

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