Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2008, Vol. 6 ›› Issue (12): 1280-1285.doi: 10.3736/jcim20081214

• Original Experimental Research • Previous Articles     Next Articles

Mechanism of Yiqi Huayu Bushen Recipe in treating cervical syndrome with kidney deficiency in rats

Jian-chun Jiang, Chen-guang Li, Quan Zhou, Qin Bian, Qian-qian Liang, Xue-jun Cui, Sheng Lu, Chong-jian Zhou, Qi Shi, Yong-jun Wang()   

  1. Institute of Spine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2008-05-30 Online:2008-12-20 Published:2008-12-15

Objective: 

To research the action mechanism of Yiqi Huayu Bushen Recipe (YHBR), a compound of traditional Chinese herbal medicine, in treating cervical syndrome (CS) with kidney deficiency in rats.


Methods:

A total of 30 three-month-old female Sprague-Dawley rats were randomly divided into normal control group, CS with kidney deficiency model group (untreated group) and YHBR group, with 10 rats in each group. Rats in the normal control group received no treatment, while rats of the other two groups underwent resection of both cervical muscles and ovaries to establish the model of CS with kidney deficiency. Three months after surgery, rats in the YHBR group were intragastricly administered YHBR for one month. Another one month later, all rats were sacrificed. The content of serum estradiol (E2) was detected by radio-immunoassay; contents of plasma cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) were tested, and the ratio of cAMP/cGMP was also calculated. Hemorheology was detected by Weissenberg's method; expression of alpha-granular membrane protein (CD62p) was detected by flow cytometry; HE staining was used to detect the histopathology of cervical intervertebral disc degeneration; type Ⅱ collagen protein was detected by immunohistochemistry and aggrecan-1 (Agc1), type Ⅱ procollagen gene, Col2a1, tissue inhibitor of metalloproteinase-1 (TIMP-1) and matrix metalloproteinase-13 (MMP-13) mRNAs were detected by fluorescent quantization polymerase chain reaction.


Results: 

Compared with the untreated group, rats in the YHBR group showed an obvious increase in serum E2 content (P<0.05), and an increase in plasma cAMP and cGMP content without significant difference; hemorheological parameters and percentage of CD62p expression were significantly decreased in the YHBR group (P<0.05, P<0.01). YHBR could improve the degeneration of cervical intervertebral discs, decrease the Miyamoto scores (P<0.05), and increase the type Ⅱ collagen. The expressions of Agc1, Col2a1 and TIMP-1 mRNAs were significantly increased and MMP-13 mRNA significantly decreased in YHBR group (P<0.05, P<0.01).


Conclusion: 

YHBR may improve CS with kidney deficiency and delay the degeneration of cervical intervertebral disc by regulating the immune-metabolism system, coagulation system and endocrine system.

Key words: Yiqi Huayu Bushen Recipe, kidney deficiency, cervical syndrome, rats

CLC Number: 

  • R681.531

Table 1

Weight of uterus and appendages in different groups (x±s, g)"

Group n Weight of uterus and appendages
Normal control 8 1.16±0.15
Untreated 8 0.33±0.02**
YHBR 8 0.32±0.03**

Table 2

Content of serum E2 in different groups (x±s, μg/L)"

Group n E2
Normal control 8 4.85±0.86
Untreated 7 4.33±0.64
YHBR 7 5.24±0.77

Table 3

Contents of plasma cAMP and cGMP and ratio of cAMP/cGMP in different groups (x±s )"

Group n cAMP (nmol/L) cGMP (nmol/L) cAMP/cGMP
Normal control 7 36.78±10.66 5.21±2.08 8.09±4.35
Untreated 9 18.35±10.33* 1.46±1.32 15.34±4.02
YHBR 9 23.22±14.26 2.36±1.79 14.43±8.46

Table 4

Changes of hemorheological parameters in different groups(x±s, mPa·s)"

Group n Whole blood low-shear viscosity Whole blood high-shear viscosity Reducing blood viscosity Aggregation index
Normal control 10 8.59±0.76 3.96±0.16 16.49±2.07 2.17±0.17
Untreated 9 14.49±4.80** 5.69±1.04** 30.10±10.32** 2.33±0.37
YHBR 9 10.63±1.64 4.97±0.62* 22.44±2.68 2.13±0.11

Table 5

Expression of CD62p in different groups (x±s, %)"

Group n CD62p
Normal control 8 5.20±3.42
Untreated 9 27.04±4.58**
YHBR 8 23.04±3.20**△△

Figure 1

HE staining of cervical intervertebral disc (Light microscopy, ×40) A: Normal control group; B: Untreated group; C: YHBR group."

Table 6

Miyamoto scores of cervical intervertebral disc in different groups (x±s )"

Group n Miyamoto score
Normal control 8 1.63±0.52
Untreated 8 4.25±0.46*
YHBR 8 3.38±0.92*

Figure 2

Immunohistochemical staining of type Ⅱ collagen in annulus fibrosus of cervical intervertebral disc (Light microscopy, ×200) A: Normal control group; B: Untreated group; C: YHBR group."

Table 7

Expressions of Agc1, Col2a1, MMP-13 and TIMP-1 mRNAs in cervical intervertebral disc in three groups(x±s)"

Group n Agc1/β-actin Col2a1/β-actin MMP-13/β-actin TIMP-1/β-actin
Normal control 6 1.00±0.34 1.00±0.48 1.00±0.84 1.00±0.46
Untreated 6 0.53±0.28* 0.11±0.04** 5.37±1.31** 0.53±0.34
YHBR 6 1.03±0.22 1.67±0.18△△ 0.50±0.09△△ 1.48±0.16
[1] Jiang JC, Li CG, Liang QQ , et al. Establishment of a rat model of cervical syndrome with kidney deficiency[J]. Zhong Xi Yi Jie He Xue Bao, 2008,6(10):1034-1039
江建春, 李晨光, 梁倩倩 , 等. 大鼠肾虚型颈椎病模型的建立[J]. 中西医结合学报, 2008,6(10):1034-1039
[2] Shi XY. Modern medical experimental zoology[M]. Beijing: People's Military Medical Press,2000: 335
施新猷 . 现代医学实验动物学[M]. 北京: 人民军医出版社,2000: 335
[3] Whitehill R, Monran DJ, Fechner RE , et al. Cervical ligamentous instability in a canine in vivo model[J]. Spine, 1987,12(10):959-963
doi: 10.1097/00007632-198712000-00001 pmid: 3441821
[4] Liu HQ, Qin JJ . Discussion on the relationship between renal deficiency blood stasis and postmenopausal osteoporosis[J]. Zhong Yi Yao Dao Bao, 2006,12(10):3-4, 29
doi: 10.3969/j.issn.1672-951X.2006.10.002
刘海全, 秦佳佳 . 论肾虚血瘀与绝经后骨质疏松症的关系[J]. 中医药导报, 2006,12(10):3-4, 29
doi: 10.3969/j.issn.1672-951X.2006.10.002
[5] Zhang SY, Shi SJ, Pu HS , et al. Effect of polysaccharide from the annual Astragalus on the proliferation of human peripheral T lymphocytes[J]. Shi Zhen Guo Yi Guo Yao, 2007,18(1):117-118
doi: 10.3969/j.issn.1008-0805.2007.01.061
张善玉, 施溯筠, 朴惠顺 , 等. 一年生栽植黄芪中黄芪多糖对人外周血T细胞增殖的影响[J]. 时珍国医国药, 2007,18(1):117-118
doi: 10.3969/j.issn.1008-0805.2007.01.061
[6] Li RJ, Qiu SD, Chen HX , et al. Immunomodulatory effects of Astragalus polysaccharide in diabetic mice[J]. Zhong Xi Yi Jie He Xue Bao, 2008,6(2):166-170
李如江, 邱曙东, 陈红霞 , 等. 黄芪多糖对1型糖尿病小鼠的免疫调节作用[J]. 中西医结合学报, 2008,6(2):166-170
[7] Kang H, Ahn KS, Cho C , et al. Immunomodu1atory effect of Astragali Radix extract on murine TH1/TH2 cell lineage deve1opment[J]. Biol Pharm Bull, 2004,27(12):1946-1950
doi: 10.1248/bpb.27.1946 pmid: 15577210
[8] Fan YG, Liu JR, Zeng ZP . Research advances in therapeutic angiogenesis[J]. Zhongguo Yao Wu Yu Lin Chuang, 2003,3(3):178-181
doi: 10.3969/j.issn.1671-2560.2003.03.005
樊粤光, 刘建仁, 曾展鹏 . 治疗性血管生成的研究进展[J]. 中国药物与临床, 2003,3(3):178-181
doi: 10.3969/j.issn.1671-2560.2003.03.005
[9] Shu B, Zhou CJ, Ma YH , et al. Research progress on pharmacological activities of the available compositions in Chinese medicinal herb Ligusticum chuanxiong[J]. Zhongguo Yao Li Xue Tong Bao, 2006,22(9):1043-1047
舒冰, 周重建, 马迎辉 , 等. 中药川芎中有效成分的药理作用研究进展[J]. 中国药理学通报, 2006,22(9):1043-1047
[10] Glazier MG, Bowman MA . A review of the evidence for the use of phytoestrogens as a replacement for traditional estrogen replacement therapy[J]. Arch Intern Med, 2001,161(9):1161-1172
doi: 10.1001/archinte.161.9.1161
[1] Bing-rong Li, Shi-yun Shao, Long Yuan, Ru Jia, Jian Sun, Qing Ji, Hua Sui, Li-hong Zhou, Yi Zhang, Hui Liu, Qi Li, Yan Wang, Bi-meng Zhang. Effects of mild moxibustion on intestinal microbiome and NLRP3 inflammasome in rats with 5-fluorouracil-induced intestinal mucositis. Journal of Integrative Medicine, 2021, 19(2): 144-157.
[2] Sitthichai Iamsaard, Supatcharee Arun, Jaturon Burawat, Supataechasit Yannasithinon, Saranya Tongpan, Sudtida Bunsueb, Natthapol Lapyuneyong, Pannawat Choowong-in, Nareelak Tangsrisakda, Chadaporn Chaimontri, Wannisa Sukhorum. Evaluation of antioxidant capacity and reproductive toxicity of aqueous extract of Thai Mucuna pruriens seeds. Journal of Integrative Medicine, 2020, 18(3): 265-273.
[3] Morufu Eyitayo Balogun, Elizabeth Enohnyaket Besong, Jacinta Nkechi Obimma, Ogochukwu Sophia Mbamalu, Fankou Serges Athanase Djobissie. Protective roles of Vigna subterranea (Bambara nut) in rats with aspirin-induced gastric mucosal injury. Journal of Integrative Medicine, 2018, 16(5): 342-349.
[4] Lucky Legbosi Nwidu, Raphael Ellis Teme. Hot aqueous leaf extract of Lasianthera africana (Icacinaceae) attenuates rifampicin-isoniazid-induced hepatotoxicity. Journal of Integrative Medicine, 2018, 16(4): 263-272.
[5] Kylie Connolly, Douglas Jackson, Candice Pullen, Andrew Fenning. Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenaline-induced vascular contraction in Wistar rats. Journal of Integrative Medicine, 2015, 13(3): 194-200.
[6] Udhaya Lavinya Baskaran, Sherry Joseph Martin, Rasool Mahaboobkhan, Sabina Evan Prince. Protective role of Triphala, an Indian traditional herbal formulation, against the nephrotoxic effects of bromobenzene in Wistar albino rats. Journal of Integrative Medicine, 2015, 13(2): 115-121.
[7] Hussein O. B. Oloyede, Matthew C. Adaja, Taofeek O. Ajiboye, Musa O. Salawu. Anti-ulcerogenic activity of aqueous extract of Carica papaya seed on indomethacin-induced peptic ulcer in male albino rats. Journal of Integrative Medicine, 2015, 13(2): 105-114.
[8] Anirudha A. Lande, Shirishkumar D. Ambavade, Uma S. Swami, Prafulla P. Adkara Prashant D. Ambavade, Arun B. Waghamare. Saponins isolated from roots of Chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase. Journal of Integrative Medicine, 2015, 13(1): 25-33.
[9] Elizabeth Abidemi Balogun, Sylvia Orume Malomo, Joseph Oluwatope Adebayo, Ahmed Adebayo Ishola, Ayodele Olufemi Soladoye, Lawrence Aderemi Olatunji, Olatunji Matthew Kolawole, Stephen Olubunmi Oguntoye, Abiola Samuel Babatunde, Oluwole Busayo Akinola . In vivo antimalarial activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf red variety) husk fibre. Journal of Integrative Medicine, 2014, 12(6): 504-511.
[10] Urmila Aswar, Mayuri Gurav, Ganesh More, Khaled Rashed, Manoj Aswar. Effect of aqueous extract of Solanum xanthocarpum Schrad. & Wendl. on postmenopausal syndrome in ovariectomized rats. Journal of Integrative Medicine, 2014, 12(5): 439-446.
[11] Xin-fang Zhang, Ji Zhu, Wen-ye Geng, Shu-jun Zhao, Chuan-wei Jiang, Sheng-rong Cai, Miao Cheng, Chuan-yun Zhou, Zi-bing Liu. Electroacupuncture at Feishu (BL13) and Zusanli (ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease. Journal of Integrative Medicine, 2014, 12(5): 417-424.
[12] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[13] Upendarrao Golla, Praveen Kumar Gajam, Solomon Sunder Bhimathati. Evaluation of diuretic and laxative activity of hydro-alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. Journal of Integrative Medicine, 2014, 12(4): 372-378.
[14] Qian Zhang, Xin-hua Xiao, Ming Li, Wen-hui Li, Miao Yu, Hua-bing Zhang, Fan Ping, Zhi-xin Wang, Jia Zheng ​. Chromium-containing traditional Chinese medicine, Tianmai Xiaoke Tablet improves blood glucose through activating insulin-signaling pathway and inhibiting PTP1B and PCK2 in diabetic rats. Journal of Integrative Medicine, 2014, 12(3): 162-170.
[15] Amir Rashidian​, Saeed Mehrzadi, Ali Reza Ghannadi, Parvin Mahzooni, Samira Sadr, Mohsen Minaiyan​. Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: A light microscopic evaluation. Journal of Integrative Medicine, 2014, 12(2): 115-120.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Wei-xiong Liang. Problems-solving strategies in clinical treatment guideline for traditional Chinese medicine and integrative medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 1-4
[2] Zhao-guo Li. Discussion on English translation of commonly used sentences in traditional Chinese medicine: part one. Journal of Chinese Integrative Medicine, 2008, 6(1): 107-110
[3] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[4] Xue-mei Liu, Qi-fu Huang, Yun-ling Zhang, Jin-li Lou, Hong-sheng Liu, Hong Zheng. Effects of Tribulus terrestris L. saponion on apoptosis of cortical neurons induced by hypoxia-reoxygenation in rats. Journal of Chinese Integrative Medicine, 2008, 6(1): 45-50
[5] . Uniform requirements for manuscripts submitted to biomedical journals: Writing and editing for biomedical publication (Chinese version, part two). Journal of Chinese Integrative Medicine, 2010, 8(11): 1001-1005
[6] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[7] Hong Liu , Guo-liang Zhang, Li Shen , Zhen Zeng, Bao-luo Zhou, Cheng-hai Liu, Guang Nie . Application and evaluation of a pseudotyped virus assay for screening herbs for anti-H5Nl avian influenza virus. Journal of Chinese Integrative Medicine, 2010, 8(11): 1036-1040
[8] Zhao-guo Li . A discussion of English translation of 1995 and 1997 Chinese National Standards of Traditional Chinese Medical Terminologies for Clinical Diagnosis and Treatment. Journal of Chinese Integrative Medicine, 2010, 8(11): 1090-1096
[9] Rui Jin, Bing Zhang. A complexity analysis of Chinese herbal property theory: the multiple formations of herbal property (Part 1). Journal of Chinese Integrative Medicine, 2012, 10(11): 1198-1205
[10] Hui-min Liu, Xian-bo Wang, Yu-juan Chang, Li-li Gu. Systematic review and meta-analysis of randomized controlled trials of integrative medicine therapy for treatment of chronic severe hepatitis. Journal of Chinese Integrative Medicine, 2012, 10(11): 1211-1228