Search JIM Advanced Search

Journal of Chinese Integrative Medicine ›› 2010, Vol. 8 ›› Issue (5): 448-452.doi: 10.3736/jcim20100508

• Original Experimental Research • Previous Articles     Next Articles

Effects of astragalosides and Panax notoginseng saponins combination on oxidative stress of cerebral ischemic reperfusion injury in mice

Hua Tan, Xiao-ping Huang, Chang-qing Deng   

  1. Laboratory of Pathophysiology, Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China
  • Received:2010-01-06 Accepted:2010-03-26 Online:2010-05-20 Published:2010-05-15
  • Contact: Chang-qing Deng


To investigate the influence of astragalosides (AST) and Panax notoginseng saponins (PNS) combination on oxidative stress of brain tissues in C57BL/6 mice with cerebral ischemia-reperfusion injury.

Eighty C57BL/6 mice were randomly divided into sham-operated group, untreated group, high-dose combination group (AST at a dose of 220 mg/kg plus PNS at a dose of 230 mg/kg), medium-dose combination group (AST at a dose of 110 mg/kg plus PNS at a dose of 115 mg/kg), low-dose combination group (AST at a dose of 55 mg/kg plus PNS at a dose of 57.5 mg/kg), AST (110 mg/kg) group, PNS (115 mg/kg) group and edaravone (4 mg/kg) group. AST and PNS were administered by gavage once daily for 4 days and edaravone was administered by intraperitoneal injection twice daily for 4 days. On the fourth day, bilateral common carotid arteries were ligated for 20 minutes to induce cerebral ischemia, followed by 60 minutes of reperfusion. Ischemic brain tissue was used to prepare tissue homogenate, then contents of malonaldehyde (MDA), glutathione (GSH) and nitric oxide (NO), and activities of superoxide dismutase (SOD) and nitric oxide synthase (NOS) in the homogenate were detected. Two×two analysis of variance of factorial design was used to analyze whether there was an interaction between AST at 110 mg/kg and PNS at 115 mg/kg.

Compared with sham-operated group, contents of MDA and NO, and activity of NOS in the untreated group were remarkably increased (P<0.01), activity of SOD and content of GSH were decreased (P<0.01). Compared with the untreated group, content of MDA in the AST group was decreased (P<0.01) and activity of SOD was increased (P<0.01), however, contents of GSH and NO and activity of NOS had no obvious changes (P>0.05). Contents of MDA and NO in the PNS group was decreased as compared with the untreated group (P<0.01), but activities of SOD and NOS and content of GSH had no changes (P>0.05). Contents of MDA and NO and activity of NOS in brain tissues in the edaravone group were decreased (P<0.01, P<0.05), and activity of SOD was increased (P<0.05), while content of GSH had no changes (P>0.05). Contents of MDA and NO and activity of NOS in brain tissue in the AST and PNS combination groups were decreased (P<0.01, P<0.05), the activity of SOD increased (P<0.01, P<0.05), the content of GSH increased (P<0.01, P<0.05), and activity of SOD and content of GSH were increased (P<0.01, P<0.05). The results of analysis of variance of factorial design showed that there were interactions between AST (110 mg/kg) and PNS (115 mg/kg) (P<0.01).

Combination of AST (110 mg/kg) and PNS (115 mg/kg) has a restraint effect on the early oxidative stress injury in the brain after ischemia-reperfusion, and the combination has a synergistic or additive effect.

Key words: Astragalosides, Panax Notoginseng saponins, Cerebral ischemia, Reperfusion injury, Oxidative stress, C57BL/6 mice

[1] Li H, Chen G, Deng CQ . Effects of plasma containing Buyang Huanwu Decoction and its effective components and total Panax notoginseng saponins on vascular smooth muscle cell proliferation induced by platelet-derived growth factor[J]. J Chin Integr Med, 2009,7(11):1078-1085
李花, 陈刚, 邓常清 . 补阳还五汤及其有效组分和三七总皂苷含药血浆抗血小板衍生生长因子诱导的血管平滑肌细胞增殖的作用[J]. 中西医结合学报, 2009,7(11):1078-1085
[2] Yang G, Kitagawa K, Matsushita K, Mabuchi T, Yagita Y, Yanagihara T, Matsumoto M . C57BL/6 strain is most susceptible to cerebral ischemia following bilateral common carotid occlusion among seven mouse strains: selective neuronal death in the murine transient forebrain ischemia[J]. Brain Res, 1997,752(1-2):209-218
doi: 10.1016/S0006-8993(96)01453-9
[3] Wellons JC 3rd, Sheng H, Laskowitz DT, Burkhard Mackensen G, Pearlstein RD, Warner DS . A comparison of strain-related susceptibility in two murine recovery models of global cerebral ischemia[J]. Brain Res, 2000,868(1):14-21
doi: 10.1016/S0006-8993(00)02216-2
[4] Rodrigo J , Fernández AP, Serrano J, Peinado MA, Martínez A .The role of free radicals in cerebral hypoxia and ischemia[J]. Free Radic Biol Med, 2005,39((1):26-50
doi: 10.1016/j.freeradbiomed.2005.02.010
[5] Chen X. Cardiovascular pharmacology[M]. Beijing: People's Medical Publishing House, 2003: 237-242
陈修 . 心血管药理学[M]. 北京: 人民卫生出版社, 2003: 237-242
[6] Liu PK, Grossman RG, Hsu CY, Robertson CS . Ischemic injury and faulty gene transcripts in the brain[J]. Trends Neurosci, 2001,24(10):581-588
doi: 10.1016/S0166-2236(00)01918-4
[7] Ikeda Y, Long DM . The molecular basis of brain injury and brain edema: the role of oxygen free radicals[J]. Neurosurgery, 1990,27(1):1-11
doi: 10.1227/00006123-199007000-00001
[8] Nishi T, Maier CM, Hayashi T, Saito A, Chan PH . Superoxide dismutase 1 overexpression reduces MCP-1 and MIP-1 alpha expression after transient focal cerebral ischemia[J]. J Cereb Blood Flow Metab, 2005,25(10):1312-1324
doi: 10.1038/sj.jcbfm.9600124
[9] Tokime T, Nozaki K, Sugino T, Kikuchi H, Hashimoto N, Ueda K . Enhanced poly(ADP-ribosyl)ation after focal ischemia in rat brain[J]. J Cereb Blood Flow Metab, 1998,18(9):991-997
doi: 10.1097/00004647-199809000-00008
[10] Chen CM, Yang WZ . The role of nitric oxide and nitric oxide synthase in cerebral ischemic reperfusion injury[J]. Yi Xue Zong Shu, 2005,11(12):1057-1059
陈春美, 杨卫忠 . 一氧化氮及一氧化氮合酶在脑缺血再灌注损伤中的作用[J]. 医学综述, 2005,11(12):1057-1059
[1] Gerli E.G. Herr, Fábio Goulart da Silva, Francisco José Cidral-Filho, Fabricia Petronilho, Lucinéia Gainski Danielski, Mariana Pereira de Souza Goldim, Afonso Shiguemi Inoue Salgado, Franciane Bobinski, Daniel Fernandes Martins, Eliane R. Winkelmann. Effects of the use of bioceramic wraps in patients with lower limb venous ulcers: A randomized double-blind placebo-controlled trial. Journal of Integrative Medicine, 2020, 18(1): 27-.
[2] Álisson de Carvalho Gonçalves, Einy Jéssika Siqueira Moreira, Guilherme Vannucchi Portari. Benfotiamine supplementation prevents oxidative stress in anterior tibialis muscle and heart. Journal of Integrative Medicine, 2019, 17(6): 423-429.
[3] Pranay Soni, Rajesh Choudhary, Surendra H. Bodakhe. Effects of a novel isoflavonoid from the stem bark of Alstonia scholaris against fructose-induced experimental cataract. Journal of Integrative Medicine, 2019, 17(5): 374-382.
[4] Fang-yuan Wang, Jian Jia, Huan-huan Song, Cheng-ming Jia, Chang-bo Chen, Jing Ma. Icariin protects vascular endothelial cells from oxidative stress through inhibiting endoplasmic reticulum stress. Journal of Integrative Medicine, 2019, 17(3): 205-212.
[5] Lucky Legbosi Nwidu, Yibala Ibor Oboma. Telfairia occidentalis (Cucurbitaceae) pulp extract mitigates rifampicin-isoniazid-induced hepatotoxicity in an in vivo rat model of oxidative stress. Journal of Integrative Medicine, 2019, 17(1): 46-56.
[6] Kayode E. Adewole, Joseph O. Adebayo. Antioxidant defense system induced by cysteine-stabilized peptide fraction of aqueous extract of Morinda lucida leaf in selected tissues of Plasmodium berghei-infected mice. Journal of Integrative Medicine, 2017, 15(5): 388-397.
[7] Uduak Akpan Okon, Idorenyin Udo Umoren. Comparison of antioxidant activity of insulin, Ocimum gratissimum L., and Vernonia amygdalina L. in type 1 diabetic rat model. Journal of Integrative Medicine, 2017, 15(4): 302-309.
[8] Udhaya Lavinya Baskaran, Evan Prince Sabina. Clinical and experimental research in antituberculosis drug-induced hepatotoxicity: A review. Journal of Integrative Medicine, 2017, 15(1): 27-36.
[9] Khanh Nguyen, Jean Sparks, Felix O. Omoruyi. Investigation of the cytotoxicity, antioxidative and immune-modulatory effects of Ligusticum porteri (Osha) root extract on human peripheral blood lymphocytes. Journal of Integrative Medicine, 2016, 14(6): 465-472.
[10] Benjamin Perry, Junzeng Zhang, Tarek Saleh, Yanwen Wang. Liuwei Dihuang, a traditional Chinese herbal formula, suppresses chronic inflammation and oxidative stress in obese rats. Journal of Integrative Medicine, 2014, 12(5): 447-454.
[11] Mukherjee Avinaba, Boujedaini Naoual, Rahman Khuda-Bukhsh Anisur. Homeopathic Thuja 30C ameliorates benzo(a)pyrene-induced DNA damage, stress and viability of perfused lung cells of mice in vitro. Journal of Chinese Integrative Medicine, 2013, 11(6): 397-404.
[12] Marjan Nassiri-Asl, Taghi Naserpour Farivar, Esmail Abbasi, Hamid Reza Sadeghnia, Mehdi Sheikhi, Mina Lotfizadeh, Parisa Bazahang. Effects of rutin on oxidative stress in mice with kainic acid-induced seizure. Journal of Chinese Integrative Medicine, 2013, 11(5): 337-342.
[13] Hai-yan Song, Li Zhang, Jie-lu Pan, Li-li Yang, Guang Ji. Bioactivity of five components of Chinese herbal formula Jiangzhi granules against hepatocellular steatosis. Journal of Chinese Integrative Medicine, 2013, 11(4): 262-268.
[14] Mao Zhi-min, Song Hai-yan, Yang Li-li, Liu Tao, Li Dong-fei, Zheng Pei-yong, Liu Ping, Ji Guang. Effects of the mixture of Swertia pseudochinensis Hara and Silybum marianum Gaertn extracts on CCl4-induced liver injury in rats with non-alcoholic fatty liver disease. Journal of Chinese Integrative Medicine, 2012, 10(2): 193-199.
[15] Kaulaskar Shyam, Bhutada Pravinkumar, Rahigude Anand, Jain Dilpesh, Harle Uday. Effects of naringenin on allodynia and hyperalgesia in rats with chronic constriction injury-induced neuropathic pain. Journal of Chinese Integrative Medicine, 2012, 10(12): 1482-1489.
Full text



[1] Jun Hu, Jian-ping Liu. Non-invasive physical treatments for chronic/recurrent headache. Journal of Chinese Integrative Medicine, 2008, 6(1): 31
[2] Jun Cai, Hua Wang, Sheng Zhou, Bin Wu, Hua-rong Song, Zheng-rong Xuan. Effect of Sijunzi Decoction and enteral nutrition on T-cell subsets and nutritional status in patients with gastric cancer after operation: A randomized controlled trial. Journal of Chinese Integrative Medicine, 2008, 6(1): 37-40
[3] Dong Yang, Yong-ping Du, Qing Shen, Wei Chen, Yan Yu, Guang-lei Chen. Expression of alpha-smooth muscle actin in renal tubulointerstitium in patients with kidney collateral stasis. Journal of Chinese Integrative Medicine, 2008, 6(1): 41-44
[4] Zhi-chun Jin. Problems in establishing clinical guideline for integrated traditional Chinese and Western medicine. Journal of Chinese Integrative Medicine, 2008, 6(1): 5-8
[5] Min Cheng, Qiong Feng, Shu-wen Qian, Hui Gao, Cui-qing Zhu. Preliminary assay of p-amyloid binding elements in heart-beneficial recipe. Journal of Chinese Integrative Medicine, 2008, 6(1): 68-72
[6] Hai-feng Wei, Bai-liu Ya, Ling Zhao, Cui-fei Ye, Li Zhang, Lin Li. Evaluation of tongue manifestation of blood stasis syndrome and its relationship with blood rheological disorder in a rat model of transient brain ischemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 73-76
[7] Li Zhou, Hong-xing Zhang, Ling-guang Liu, Wen-jun Wan. Effect of electro-acupuncture at Fenglong (GV 16) on nitric oxide and endothelin in rats with hyperlipidemia. Journal of Chinese Integrative Medicine, 2008, 6(1): 89-92
[8] Jin-zhou Tian, Jing Shi, Xin-qing Zhang, Qi Bi, Xin Ma, Zhi-liang Wang, Xiao-bin Li, Shu-li Shen, Lin Li, Zhen-yun Wu, Li-yan Fang, Xiao-dong Zhao, Ying-chun Miao, Peng-wen Wang, Ying Ren, Jun-xiang Yin, Yong-yan Wang, Beijing United Study Group on MCI of the Capital Foundation of Medical Developments. Guiding principles of clinical research on mild cognitive impairment (protocol). Journal of Chinese Integrative Medicine, 2008, 6(1): 9-14
[9] Daniel Weber, Janelle M Wheat, Geoffrey M Currie. Inflammation and cancer: Tumor initiation, progression and metastasis,and Chinese botanical medicines. Journal of Chinese Integrative Medicine, 2010, 8(11): 1006-1013
[10] Xin-jun Wang, Ling-ling Wang . A mechanism of endogenous opioid peptides for rapid onset of acupuncture effect in treatment of depression. Journal of Chinese Integrative Medicine, 2010, 8(11): 1014-1017